亲和仿生层析及在抗体纯化中的应用

doi:10.11949/j.issn.0438-1157.20160203

摘要/Abstract

摘要：

亲和仿生层析是一种新型的生物分离技术，可用于生物活性物质分离，尤其在抗体纯化中表现出良好的应用前景，具有价格低廉、结构稳定、特异性较高等优点。亲和仿生层析的核心是具有特定结构的仿生配基，主要包括化学合成配基和短肽配基两种，通常通过合理的手段进行筛选和设计。理性设计是一种行之有效的方法，针对一个特定的目标蛋白，随机地去选择配基是不可能的，必须采用理性设计构建一个合理的配基库，并通过高通量的筛选技术，才能得到合适的仿生配基。本文根据近年来国内外亲和仿生层析的研究进展，着重介绍了亲和仿生配基的筛选和设计以及在抗体纯化中的应用。

关键词: 亲和仿生层析, 化学合成配基, 短肽配基, 抗体, 纯化, 分子模拟

Abstract:

Affinity biomimetic chromatography is a novel bioseparation technology for the biological active substance, particularly showing good performance for antibody purification with the advantages of low cost, stable ligand structure, high specificity and so on. The critical point of affinity biomimetic chromatography is the specially-designed biomimetic ligand. The biomimetic ligands used currently include chemical synthetic ligand and short peptide ligand, which are normally obtained by rational design and screening. For a particular target protein, instead of selecting the ligand randomly, it is necessary to build a reasonable ligand library by rational designing method and screen the suitable mimetic ligand by high-throughput screening technology. According to the recent advances in affinity biomimetic chromatography, the designing and screening of ligands used for affinity mimetic chromatography and the applications in antibody purification are reviewed in the present article.

Key words: affinity biomimetic chromatography, chemical synthetic ligand, short peptide ligand, antibody, purification, molecular simulation

中图分类号:

TQ028

卢慧丽, 林东强, 姚善泾. 亲和仿生层析及在抗体纯化中的应用[J]. 化工学报, 2016, 67(9): 3523-3535.

LU Huili, LIN Dongqiang, YAO Shanjing. Affinity biomimetic chromatography and its applications for antibody purification[J]. CIESC Journal, 2016, 67(9): 3523-3535.

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