不同DO下SBBR亚硝酸型同步脱氮及N2O释放特性

doi:10.11949/j.issn.0438-1157.20181333

摘要/Abstract

摘要：

在(20±2.0)℃ 条件下，以实际生活污水为处理对象，以碳纤维为填料（填充率35%），利用序批式生物膜（sequencing batch biofilm reactor，SBBR）反应器，通过限氧曝气，成功实现了亚硝酸型同步生物脱氮（simultaneous nitrification and denitrification，SND）过程。荧光原位杂交技术（fluorescence in-situ hybridization，FISH）半定量表明，氨氧化菌（ammonia oxidizing bacteria, AOB）是硝化系统中的优势菌种。微生物将外碳源以聚β–羟基烷酸酯(poly-β-hydroxyalkanoate，PHA)的形式储存在体内，作为后续反硝化过程所需内碳源。DO=0.5 mg/L，SBBR系统NH₄ ⁺-N和TN去除率分别为95%以上和80.4%，SND效率达77.9%。出水NO _x ^--N小于10 mg/L，且以NO₂ ^--N为主。DO=2.0、1.2和0.5 mg/L时，系统N₂O释放量分别为1.38、2.39和1.65 mg/L。AOB的好氧反硝化过程和低氧条件下以PHA作为内碳源的NO _x ^--N反硝化过程，都会导致N₂O释放。低DO水平是实现亚硝酸型同步脱氮过程和降低N₂O释放的关键因素。低DO促进了AOB的竞争优势，形成了良好的缺氧微环境，降低了COD降解速率，为反硝化过程提供外碳源作为电子供体，从而降低了N₂O释放量。

关键词: 亚硝酸型同步硝化反硝化, 溶解氧, N₂O, PHA

Abstract:

At normal temperature of (20±2.0)℃, using actual domestic wastewater as influent substrate and carbon fibre as biological carriers (filling rate 35%), the simultaneous nitrification and denitrification via nitrite was achieved in a sequencing batch biofilm reactor under limited DO concentrations. The results of fluorescence in-situ hybridization (FISH) demonstrated that the ammonia oxidizing bacteria (AOB) became the dominant species in the nitrification process under limited DO concentration. During the anoxic and the initial aerobic process, the external COD was taken up and converted to polyhydroxyalkanoate (PHA), which can be used as internal carbon sources for the following denitrification. DO=0.5 mg/L, the NH₄ ⁺-N removal, TN removal and simultaneous nitrification and denitrification efficiency was more than 95%, 80.4% and 77.9%, respectively. The concentration of NO _x ^--N in the effluent was less than 10 mg/L and mainly in the form of NO₂ ^--N. When the DO concentration was controlled at 2.0, 1.2 and 0.5 mg/L, the N₂O emission was 1.38, 2.39 and 1.65 mg/L, respectively. Both the aerobic denitrification process of AOB and the denitrification of NO _x ^--N with PHA as carbon source at the presence of lower oxygen can lead to the release of N₂O. The long time of lower DO conditions promoted AOB s competitive advantage, formed a micro-environment for denitrifiers, reduced the COD degradation rate, and provided an external carbon source as an electronic donor for denitrification, thereby reducing N₂O emissions.

Key words: simultaneous nitrification and denitrification via nitrite, DO, N₂O, PHA

中图分类号:

X 703.1

巩有奎, 任丽芳, 彭永臻. 不同DO下SBBR亚硝酸型同步脱氮及N₂O释放特性[J]. 化工学报, 2019, 70(4): 1550-1558.

Youkui GONG, Lifang REN, Yongzhen PENG. Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations[J]. CIESC Journal, 2019, 70(4): 1550-1558.

图/表 9

图1 SBBR试验装置示意图

Fig.1 Schematic diagram of SBBR system

表1 不同菌群比耗氧速率（SOUR）试验过程[18]

Table 1 Process of SOUR for different bacteria

编号	添加抑制剂	实验目的	比耗氧速率（SOUR）
1	—	测定SBBR系统微生物总SOUR	SOUR₁
2	ATU	抑制AOB活性	SOUR₂
3	NaClO₃	抑制NOB活性	SOUR₃
4	ATU+ NaClO₃	同时抑制AOB和NOB的活性	SOUR₄

图2 不同DO下SBBR反应器运行特性

Fig.2 Performance of nitrogen removal in SBBR under different DO concentrations

图3 不同DO下系统AOB、NOB耗氧速率

Fig.3 Effect of DO concentration on biofilm activities in SBBR

图4 不同阶段污泥中硝化菌群相对含量

Fig.4 Enrichment nitrifying bacteria in SBBR in different periods

图5 典型周期系统内碳源利用情况

Fig.5 Utilization of carbon source and PHA in typical cycles

图6 不同DO下典型周期SBBR反应器NH4 +-N，NO2 --N，NO3 --N，N2O-N和TN变化曲线

Fig.6 Variation of NH4 +-N, NO2 --N, NO3 --N, N2O-N and TN concentrations in typical operational cycles of SBBR under different DO concentrations

图7 硝化过程与异养反硝化过程中 N2O 的产生途径[26]

Fig.7 Pathway of N2O production during nitrification and denitrification process[26]

图8 不同DO下典型周期SBBR反应器N2O产生速率

Fig.8 Variation of N2O-N production rate in typical operational cycles of SBBR under different DO concentrations

参考文献 35

1	Wang J , Rong H , Zhang C . Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor[J]. Journal of Bioscience and Bioengineering, 2018, 125(5): 532-542.
2	Zhao J , Feng L , Yang G , et al . Development of simultaneous nitrification- denitrification (SND) in biofilm reactors with partially coupled a novel biodegradable carrier for nitrogen-rich water purification[J]. Bioresource Technology, 2017, 243: 800-809.
3	Han Y , Liu J , Guo X , et al . Micro-environment characteristics and microbial communities in activated sludge flocs of different particle size[J]. Bioresource Technology, 2012, 124(11): 252-258.
4	Ma W , Han Y , Ma W , et al . Enhanced nitrogen removal from coal gasification wastewater by simultaneous nitrification and denitrification (SND) in an oxygen-limited aeration sequencing batch biofilm reactor[J]. Bioresource Technology, 2017, 44(11): 84-91.
5	Leonidia M C D , Eloisa P , Eugenio F , et al . Removal of ammonium via simultaneous nitrification-denitrification nitrite-shortcut in a single packed-bed batch reactor[J]. Bioresource Technology, 2009, 100(3): 1100-1107．
6	Stefano M , Giaime T , Giovannimatteo E . Evaluation of nitrous oxide gaseous emissions from a partial nitritation reactor operating under different conditions[J]. Desalination and Water Treatment, 2018, DOI: 10.5004/dwt.2018.22934. DOI URL
7	Wen Q , Chen Z , Shi H . T-RFLP detection of nitrifying bacteria in a fluidized bed reactor of achieving simultaneous nitrification- denitrification.[J]. Chemosphere, 2008, 71(9): 1683-1692.
8	Solomon S , Qin D , Manning M , et al . Climate Change 2007: the Physical Science Basis[M]. Cambridge: Cambridge University Press, 2007.
9	Ravishankara A R , Daniel J S , Portmann R W . Nitrous oxide(N₂O): the dominant ozone-depleting substance emitted in the 21st century[J]. Science, 2009, 326(5949): 123-125.
10	Kong Q , Wang Z , Niu P , et al . Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process[J]. Bioresource Technology, 2016, 210: 94-100.
11	Ye L , Ni B J , Law Y Y , et al . A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators[J]. Water Research, 2014, 48(1): 257-268.
12	巩有奎, 王赛, 彭永臻, 等 . 生活污水不同生物脱氮过程中N₂O产量及控制[J]. 化工学报, 2010, 61(5): 1286-1292.
	Gong Y K , Wang S , Peng Y Z , et al . Formation of N₂O in various biological nitrogen removal processes for treatment of domestic sewage[J]. CIESC Journal, 2010, 61(5): 1286-1292.
13	APHA(American Public Health Association) . Standard methods for the examination of water and wastewater[S]. Baltimore: Port City Press, 1998.
14	Oehmen A , Keller-Lehmann B , Zeng R J , et al . Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J]. Journal of Chromatography A, 2005, 1070(1/2): 131-136.
15	张建华, 彭永臻, 张淼, 等 .同步硝化反硝化SBBR 处理低C/N 比生活污水的启动与稳定运行[J].化工学报, 2016, 67(11): 4817-4824.
	Zhang J H , Peng Y Z , Zhang M , et al . Start-up and steady operation of simultaneous nitrification and denitrification in SBBR treating low C/N ratio domestic wastewater[J]. CIESC Journal, 2016, 67(11): 4817-4824.
16	曾薇, 杨庆, 张树军, 等 . 采用 FISH、DGGE 和 Cloning 对短程脱氮系统中硝化菌群的比较分析[J]. 环境科学学报, 2006, 26(5): 734-739.
	Zeng W , Yang Q , Zhang S J , et al . Analysis of nitrifying bacteria in short-cut nitrification- denitrification processes by using FISH, PCR-DGGE and Cloning[J]. Acta Scientiae Circumstantiae, 2006, 26(5): 734-739.
17	王建龙, 吴立波, 齐星, 等 . 用氧吸收速率( OUR) 表征活性污泥硝化活性的研究[J]. 环境科学学报, 1999, 19(3): 225-229.
	Wang J L , Wu L B , Qi X , et al . Characterization of nitrification activity of activated sludge by oxygen uptake rate (OUR) [J]. Acta Scientiae Circumstantiae, 1999, 19(3): 225-229.
18	巩有奎, 苗志加, 彭永臻 .生物脱氮好氧阶段不同反应过程N₂O产量[J].环境工程学报, 2016, 12(12): 6963-6968.
	Gong Y K , Miao Z J , Peng Y Z . Nitrous oxide production from sequencing batch reactor sludge under nitrifying conditions of different process[J]. Chinese Journal of Environmental Engineering, 2016, 12(12): 6963-6968.
19	Yilmaz G , Lemaire R , Keller J , et al . Simultaneous nitrification, denitrification, and phosphorus removal from nutrient-rich industrial wastewater using granular sludge[J]. Biotechnol. Bioeng., 2008, 100(3): 529-541.
20	Rikke L M , Zeng R L , Giugliano V , et al . Challenges for simultaneous nitrification, denitrification, and phosphorus removal in microbial aggregates: mass transfer limitation and nitrous oxide production[J]. FEMS Microbiology Ecology, 2005, 52(3): 329-338.
21	Guisasola A , Jubany I , Baeza J A , et al . Respirometric estimation of the oxygen affinity constants for biological ammonia and nitrite oxidation[J]. Journal of Chemical Technology & Biotechnology, 2005, 80(4): 388-396.
22	Soliman M , Eldyasti A . Ammonia-oxidizing bacteria (AOB): opportunities and applications—a review[J]. Reviews in Environmental Science and Bio/Technology, 2018, DOI: 10.1007/s11157-018-9463-4. DOI URL
23	Bernet N , Dangcong P , Delgenes J P , et al . Nitrification at low oxygen concentration in biofilm reactor [J]. Journal of Environmental Engineering-ASCE, 2001, 127 (3): 266-271.
24	Laanbroek H J , Gerards S . Competition for limiting amounts of oxygen between Nitrosomonas europaea and Nitrobacter winogradskyi grown in mixed continuous cultures [J]. Archives of Microbiology, 1993, 159(5): 453-459.
25	曾薇, 张悦, 李磊 . 限氧曝气实现亚硝酸型同步硝化反硝化的研究[J]. 北京工业大学学报, 2010, 36(9): 1263-1270.
	Zeng W , Zhang Y , Li L . Simultaneous nitrification denitrification via nitrite under limited aeration[J]. Journal of Beijing University of Technology, 2010, 36(9): 1263-1270.
26	Law Y , Ye L , Pan Y , et al . Nitrous oxide emissions from wastewater treatment processes [J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 2012, 367(1593): 1265-1277.
27	Yang Q , Liu X H , Peng C Y , et al . N₂O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method[J]. Environmental Science & Technology, 2009, 43(24): 9400-9406.
28	Pan Y T , Ni B J , Yuan Z G . Modeling electron competition among nitrogen oxides reduction and N₂O accumulation in denitrification[J]. Environmental Science & Technology, 2013, 47(19): 11083-11091.
29	巩有奎, 王淑莹, 王莎莎 . 碳氮比对短程反硝化过程中N₂O产生的影响[J].化工学报, 2011, 62(7): 2049-2054.
	Gong Y K , Wang S Y , Wang S S . Effect of C/N ratio on N₂O accumulation and reduction during nitrite denitrification process[J]. CIESC Journal, 2011, 62(7): 2049-2054.
30	Pan Y T , Ye L , Ni J B , et al . Effect of pH on N₂O reduction and accumulation during denitrification by methanol utilizing denitrifiers[J]. Water Research, 2012, 46(15): 4832-4840.
31	Zhao W , Wang Y , Liu S , et al . Denitrification activities and N₂O production under salt stress with varying CODN ratios and terminal electron acceptors[J]. Chemical Engineering Journal, 2013, 215/216(2): 252-260.
32	Gong Y , Peng Y , Yang Y . Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate[J]. Journal of Hazardous Materials, 2012, 227/228: 453-460.
33	Pan Y , Ni B , Lu H , et al . Evaluating two concepts for the modelling of intermediates accumulation during biological denitrification in wastewater treatment[J]. Water Research, 2015, 71(15): 21-31.
34	Chen X , Yuan Z , Ni B J . Nitrite accumulation inside sludge flocs significantly influencing nitrous oxide production by ammonium-oxidizing bacteria[J]. Water Research, 2018, 143(15): 99-108.
35	郑桂林, 张朝升, 曹勇锋, 等 . DO在SBBR生物膜中传递及对同步硝化反硝化的影响[J]. 中国给水排水, 2016, 32(3): 22-26.
	Zheng G L , Zhang C S , Cao Y F , et al . Transfer of DO in biofilm and effect of DO on simultaneous nitrification and denitrification in sequencing batch biofilm reactor [J]. China Water＆Wastewater, 2016, 32(3): 22-26.

编辑推荐 0

Metrics

阅读次数

全文

240

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	4	0	0	236

来源	本网站	其他网站

次数	161	79
比例	67%	33%

摘要

530

最新录用	在线预览	正式出版

0	0	530

来源	本网站	其他网站

次数	370	160
比例	70%	30%

[1]	杨菲菲, 赵世熙, 周维, 倪中海. Sn掺杂的In₂O₃催化CO₂选择性加氢制甲醇[J]. 化工学报, 2023, 74(8): 3366-3374.
[2]	徐振和, 李泓江, 高雨, 礼峥, 张含烟, 徐宝彤, 丁茯, 孙亚光. In₂O₃/Ag：ZnIn₂S₄“Type Ⅱ”型异质结构材料的制备及可见光催化性能[J]. 化工学报, 2022, 73(8): 3625-3635.
[3]	巩有奎, 罗佩云, 孙洪伟. 厌氧-限氧SBR处理低C/N生活污水SNDPR启动及N₂O释放[J]. 化工学报, 2021, 72(8): 4381-4390.
[4]	徐宇峰, 郭鸣, 王让, 肖伟, 刘元慧, 李思敏. 复合改性生物砂滤池对突发PhACs痕量污染的去除效果分析[J]. 化工学报, 2020, 71(7): 3322-3332.
[5]	巩有奎, 彭永臻. 运行方式对SBBR亚硝酸型同步脱氮及N₂O释放的影响[J]. 化工学报, 2019, 70(6): 2289-2297.
[6]	马双忱, 范紫瑄, 万忠诚, 陈嘉宁, 张净瑞, 马采妮. 高盐水条件下亚硫酸盐氧化特性实验研究[J]. 化工学报, 2019, 70(5): 1964-1972.
[7]	巩有奎, 赵强, 彭永臻. 不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化[J]. 化工学报, 2019, 70(12): 4847-4855.
[8]	巩有奎, 彭永臻. 曝气强度对SBBR同步生物脱氮及N₂O释放影响[J]. 化工学报, 2019, 70(11): 4410-4419.
[9]	仇媛, 王长真, 李海涛, 胡晓波, 王永钊, 赵永祥. 单分散Co₃O₄@SiO₂核壳催化剂的制备及N₂O催化分解性能[J]. 化工学报, 2018, 69(4): 1493-1499.
[10]	韩红桂, 刘峥, 乔俊飞. 基于区间二型模糊神经网络污水处理过程溶解氧浓度控制[J]. 化工学报, 2018, 69(3): 1182-1190.
[11]	周红标. 基于自组织模糊神经网络的污水处理过程溶解氧控制[J]. 化工学报, 2017, 68(4): 1516-1524.
[12]	张倩, 王淑莹, 苗圆圆, 王晓霞, 彭永臻. 间歇低氧曝气下CANON工艺处理生活污水的启动[J]. 化工学报, 2017, 68(1): 289-296.
[13]	余岳溪, 高正阳, 季鹏, 李方勇, 杨维结. 煤焦异相还原N₂O的反应机理[J]. 化工学报, 2017, 68(1): 369-374.
[14]	谢丽, 殷紫, 尹志轩, 王悦超, 周琪. 一段式厌氧氨氧化工艺亚硝酸盐氧化菌抑制方法研究进展[J]. 化工学报, 2016, 67(7): 2647-2655.
[15]	崔有为, 林小媛, 冀思远, 施云鹏. SRT对富集高聚PHA能力嗜盐MMC的影响[J]. 化工学报, 2016, 67(6): 2575-2582.

不同DO下SBBR亚硝酸型同步脱氮及N₂O释放特性

Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 35

相关文章 15

编辑推荐 0

Metrics

本文评价