CIESC Journal ›› 2019, Vol. 70 ›› Issue (10): 3690-3703.DOI: 10.11949/0438-1157.20190591
• Reviews and monographs • Previous Articles Next Articles
Shanjing YAO(),Linian CAI,Dongqiang LIN
Received:
2019-05-29
Revised:
2019-07-02
Online:
2019-10-05
Published:
2019-10-05
Contact:
Shanjing YAO
通讯作者:
姚善泾
作者简介:
姚善泾(1957—),男,博士,教授,基金资助:
CLC Number:
Shanjing YAO, Linian CAI, Dongqiang LIN. Progress in Aspergillus niger as cell factory for secretory proteins[J]. CIESC Journal, 2019, 70(10): 3690-3703.
姚善泾, 蔡礼年, 林东强. 黑曲霉作为分泌蛋白细胞工厂的研究进展[J]. 化工学报, 2019, 70(10): 3690-3703.
参考依据 | 大肠杆菌 | 黑曲霉 | 酵母 | 昆虫细胞 | 哺乳动物细胞 | 植物细胞 |
---|---|---|---|---|---|---|
转化难易 | 简单 | 较难 | 一般 | 较难 | 较难 | 较难 |
培养周期 | 1天以内 | 1周左右 | 1周左右 | 1周左右 | 1周左右 | 1月左右 |
培养成本 | 低 | 低 | 低 | 高 | 高 | 较高 |
分泌性能 | 低 | 高 | 较高 | 高 | 高 | 高 |
蛋白折叠 | 低 | 较高 | 较高 | 高 | 高 | 高 |
N-糖基化 | 无 | 哺乳动物型的糖核 | 高比例甘露聚糖 | 与哺乳动物细胞类似 | 哺乳动物细胞类型 | 与哺乳动物细胞类似 |
O-糖基化 | 无 | 有 | 有 | 有 | 有 | 有 |
Table 1 Comparison of different cell expression systems
参考依据 | 大肠杆菌 | 黑曲霉 | 酵母 | 昆虫细胞 | 哺乳动物细胞 | 植物细胞 |
---|---|---|---|---|---|---|
转化难易 | 简单 | 较难 | 一般 | 较难 | 较难 | 较难 |
培养周期 | 1天以内 | 1周左右 | 1周左右 | 1周左右 | 1周左右 | 1月左右 |
培养成本 | 低 | 低 | 低 | 高 | 高 | 较高 |
分泌性能 | 低 | 高 | 较高 | 高 | 高 | 高 |
蛋白折叠 | 低 | 较高 | 较高 | 高 | 高 | 高 |
N-糖基化 | 无 | 哺乳动物型的糖核 | 高比例甘露聚糖 | 与哺乳动物细胞类似 | 哺乳动物细胞类型 | 与哺乳动物细胞类似 |
O-糖基化 | 无 | 有 | 有 | 有 | 有 | 有 |
酶分类 | 酶种类 | 获取方式① |
---|---|---|
纤维素酶 | 内切葡聚糖酶 | D[ |
外切葡聚糖酶 | D[ | |
β-葡萄糖苷酶 | D[ | |
裂解多糖单氧化酶 | P[ | |
半纤维素酶 | 木聚糖酶 | D[ |
木糖苷酶 | D[ | |
甘露聚糖酶 | P[ | |
甘露糖苷酶 | P[ | |
乙酰木聚糖酯酶 | P[ | |
果胶酶 | 果胶甲酯酶 | P[ |
果胶酶 | D[ | |
聚半乳糖醛酸酶 | D[ | |
果胶裂解酶 | D[ | |
淀粉酶 | 葡糖淀粉酶 | D[ |
淀粉酶 | D[ | |
蛋白酶 | 肽酶 | D[ |
角质蛋白酶 | P[ | |
酸性蛋白酶 | D[ | |
氨肽酶 | P[ | |
其他酶 | 脂肪酶 | D[ |
α-葡萄糖苷酶 | D[ | |
植酸酶 | D[ | |
芸香苷酶 | P[ | |
阿魏酸酯酶 | P[ | |
鞣酸酶 | D[ | |
菊粉酶 | E[ | |
天冬酰胺酶 单宁酶 | D[ A[ |
Table 2 Secretases of A. niger
酶分类 | 酶种类 | 获取方式① |
---|---|---|
纤维素酶 | 内切葡聚糖酶 | D[ |
外切葡聚糖酶 | D[ | |
β-葡萄糖苷酶 | D[ | |
裂解多糖单氧化酶 | P[ | |
半纤维素酶 | 木聚糖酶 | D[ |
木糖苷酶 | D[ | |
甘露聚糖酶 | P[ | |
甘露糖苷酶 | P[ | |
乙酰木聚糖酯酶 | P[ | |
果胶酶 | 果胶甲酯酶 | P[ |
果胶酶 | D[ | |
聚半乳糖醛酸酶 | D[ | |
果胶裂解酶 | D[ | |
淀粉酶 | 葡糖淀粉酶 | D[ |
淀粉酶 | D[ | |
蛋白酶 | 肽酶 | D[ |
角质蛋白酶 | P[ | |
酸性蛋白酶 | D[ | |
氨肽酶 | P[ | |
其他酶 | 脂肪酶 | D[ |
α-葡萄糖苷酶 | D[ | |
植酸酶 | D[ | |
芸香苷酶 | P[ | |
阿魏酸酯酶 | P[ | |
鞣酸酶 | D[ | |
菊粉酶 | E[ | |
天冬酰胺酶 单宁酶 | D[ A[ |
蛋白来源 | 蛋白种类 | 表达水平① | 参考文献 |
---|---|---|---|
人 | α1蛋白酶抑制剂 | 12 mg/L | [ |
人 | 白细胞介素-6 | 15 mg/L | [ |
人 | 溶菌酶 | 40 mg/L | [ |
马 | 溶菌酶 | 150 mg/L | [ |
牛 | 肠激酶 | 5 mg/L | [ |
利什曼虫 | 抗原蛋白 | 54 mg/L | [ |
福寿螺 | 纤维素酶 | SDS-PAGE | [ |
杏鲍菇 | 过氧物酶 | 466 U/L | [ |
云芝 | 漆酶 | 2700 U/L | [ |
黄孢原毛平革菌 | 锰过氧物酶 | 100 mg/L | [ |
黄孢原毛平革菌 | 木质素过氧物酶 | Western blot | [ |
里氏木霉 | 木聚糖酶,内切葡聚糖酶 | SDS-PAGE | [ |
嗜热子囊菌,里氏木霉 | 29个纤维素酶 | SDS-PAGE | [ |
白曲霉 | 酸性蛋白酶 | 9972 U/ml | [ |
米黑根毛霉 | 脂肪酶 | 15 U/ml | [ |
梨囊鞭菌属R | 纤维素酶 | SDS-PAGE | [ |
朱红密孔菌 | 漆酶 | SDS-PAGE | [ |
Caldariomyces fumago | 氯化物过氧物酶 | SDS-PAGE | [ |
海栖热袍菌 | 木聚糖酶 | 500 mg/L | [ |
解淀粉芽孢杆菌 | 纤维素酶 | SDS-PAGE | [ |
Table 3 Proteins heterologously expressed in A. niger
蛋白来源 | 蛋白种类 | 表达水平① | 参考文献 |
---|---|---|---|
人 | α1蛋白酶抑制剂 | 12 mg/L | [ |
人 | 白细胞介素-6 | 15 mg/L | [ |
人 | 溶菌酶 | 40 mg/L | [ |
马 | 溶菌酶 | 150 mg/L | [ |
牛 | 肠激酶 | 5 mg/L | [ |
利什曼虫 | 抗原蛋白 | 54 mg/L | [ |
福寿螺 | 纤维素酶 | SDS-PAGE | [ |
杏鲍菇 | 过氧物酶 | 466 U/L | [ |
云芝 | 漆酶 | 2700 U/L | [ |
黄孢原毛平革菌 | 锰过氧物酶 | 100 mg/L | [ |
黄孢原毛平革菌 | 木质素过氧物酶 | Western blot | [ |
里氏木霉 | 木聚糖酶,内切葡聚糖酶 | SDS-PAGE | [ |
嗜热子囊菌,里氏木霉 | 29个纤维素酶 | SDS-PAGE | [ |
白曲霉 | 酸性蛋白酶 | 9972 U/ml | [ |
米黑根毛霉 | 脂肪酶 | 15 U/ml | [ |
梨囊鞭菌属R | 纤维素酶 | SDS-PAGE | [ |
朱红密孔菌 | 漆酶 | SDS-PAGE | [ |
Caldariomyces fumago | 氯化物过氧物酶 | SDS-PAGE | [ |
海栖热袍菌 | 木聚糖酶 | 500 mg/L | [ |
解淀粉芽孢杆菌 | 纤维素酶 | SDS-PAGE | [ |
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