Recent developments in enzymatic reactive distillation coupling technology

doi:10.11949/0438-1157.20191232

Abstract

Abstract:

Enzymatic reactive distillation which combines the enzyme-catalyzed reaction with distillation process can be used to break the limitation of reaction equilibrium and improve the conversion and selectivity effectively. It is a new process intensification technology. In this paper, the progress of enzymatic reactive distillation coupling technology is reviewed from the enzyme catalyst and its catalytic reaction mechanism, the packing method of enzyme catalyst, the coupling mode of enzyme reaction and distillation process and several application cases. It shows that current development of enzyme reactive distillation coupling technique is not mature. The enzyme-catalyzed reactive distillation is quite different with the reactive distillation catalyzed by chemical catalyst. And the influences of parameters such as reaction temperature, substrate concentration on enzyme catalytic activity should be considered. In the future, the strengthening research can be carried out from the research system, enzyme immobilization technology, enzyme catalyst loading mode, enzyme reactive distillation theory research, enzyme reactive distillation coupling process development and so on.

Key words: enzyme, catalysis, reactive distillation, process intensification

摘要：

酶反应精馏是将酶催化反应与精馏过程进行耦合，可有效打破反应化学平衡的限制，提高酶反应转化率和选择性，是一种新型化工过程强化技术。本文分别从酶催化剂及其催化反应机制、酶催化剂装填方式、酶催化反应与精馏耦合型式、酶反应精馏耦合技术的应用案例等方面综述了酶反应精馏耦合技术的研究进展，分析表明：目前酶反应精馏耦合技术的开发尚不成熟，且与化学催化剂催化的反应精馏过程不同，酶反应精馏工艺过程开发还需考虑酶催化反应温度、底物浓度等因素对酶催化活性影响。在后续研究中，可分别从研究体系、酶的固定化技术、酶催化剂装填方式、酶反应精馏理论研究、酶反应精馏耦合工艺开发等方面开展强化研究。

关键词: 酶, 催化, 反应精馏, 过程强化

CLC Number:

Q 814

Qinglian WANG, Xiaoda WANG, Hongxing WANG, Zhixian HUANG, Changshen YE, Ting QIU. Recent developments in enzymatic reactive distillation coupling technology[J]. CIESC Journal, 2020, 71(1): 122-137.

王清莲, 王晓达, 王红星, 黄智贤, 叶长燊, 邱挺. 酶反应精馏耦合技术研究进展[J]. 化工学报, 2020, 71(1): 122-137.

Figures/Tables 22

Fig.1 3D structure diagram of lipase

Fig.2 Combination mode of enzymatic and substrate

Fig.3 Diagram of reaction mechanism of double substrate reaction A+BP+Q

Fig.4 Reactive mechanism of hydrolysis reaction catalyzed by lipase

Fig.5 Diagram of packed structured enzyme catalytic packing

Fig.6 Commercial gauze packing before and after coating lipase CalB

Fig.7 Pilot plant of enzymatic reactive dividing wall distillation

Fig.8 Scheme of ultrasound-assisted enzymatic reactive distillation process

Fig.9 Schematic diagram of side-reactor reactive distillation process

Fig.10 Reaction equation of transesterification of ethyl butyrate with n-butanol

Fig.11 Operating window of enzymatic reactive distillation for transesterification of ethyl butyrate

Fig.12 Polit-scale process equipment of preparation of butyl butyrate by enzymatic reactive distillation

Fig.13 Concentration and temperature profiles inside enzymatic reactive distillation column for transesterification of ethyl butyrate

Fig.14 Reaction equation of kinetic resolution of racemic (R/S)-1-phenylethanol

Fig.15 Operating window of enzymatic reactive distillation for kinetic resolution of racemic (R/S)-1-phenylethanol

Fig.16 Concentration and temperature profiles inside enzymatic reactive distillation column for kinetic resolution of racemic (R/S)-1-phenylethanol

Fig.17 Scheme of batch reactive distillation setup of kinetic resolution of racemic (R/S)-2-pentanol

Fig.18 Reaction equation of kinetic resolution of racemic (R/S)-2-pentanol

Fig.19 Time dependent concentration progress curves of all reaction components in the bottom and top of column for kinetic resolution of (R/S)-2-pentanol

Fig.20 Molar fractions in the top of RD column for kinetic resolution of (R/S)-2-pentanol with propyl butyrate

Fig.21 Reactive distillation miniplant of preparation of four enantiomers

Fig.22 Chiral purities and concentrations of components in reactive distillation miniplant

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