CIESC Journal ›› 2021, Vol. 72 ›› Issue (10): 5319-5329.DOI: 10.11949/0438-1157.20210378

• Biochemical engineering and technology • Previous Articles     Next Articles

Urease-driven preparation of calcium carbonate micro-nanoparticles with different polymorphs

Hui ZHOU1(),Zhifeng TIAN2,Xiaowei TANG2,Zhilong XIU1()   

  1. 1.School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China
    2.School of Civil Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China
  • Received:2021-03-15 Revised:2021-05-18 Online:2021-10-05 Published:2021-10-05
  • Contact: Zhilong XIU

脲酶驱动不同晶型碳酸钙微纳米颗粒的制备

周惠1(),田志锋2,唐小微2,修志龙1()   

  1. 1.大连理工大学生物工程学院,辽宁 大连 116024
    2.大连理工大学土木工程学院,辽宁 大连 116024
  • 通讯作者: 修志龙
  • 作者简介:周惠(1994—),女,硕士研究生,huizhou811@163.com

Abstract:

The urease produced by Sporosarcina pasteurii can hydrolyze urea, and the generated CO2 reacts with calcium chloride to obtain calcium carbonate with different polymorphs by urease in different solutions. The mesoporous hollow micron pure spheroids formed by self-assembly of nanospheres were obtained by urease in the supernatant of fermentation broth. Calcite with 100% was obtained by bacterial urease. Infrared spectrum analysis showed that the elliptical calcium carbonate obtained by crude urease was affected by the hydroxyl groups. The effects of urease activity and reactant concentration on vaterite were explored. It was also found that the obtained pure vaterite is very stable within 7 days and is expected to be used as a drug carrier.

Key words: urease, enzyme, catalysis, vaterite, calcite, nanoparticles

摘要:

巴氏芽孢八叠球菌(Sporosarcina pasteurii)产生的脲酶可水解尿素,生成的CO2与氯化钙反应获得碳酸钙晶体,不同形式的脲酶溶液对碳酸钙晶型有显著影响。用发酵液上清液中的脲酶催化,可以获得由纳米级球霰石自组装成的介孔空心微米纯球霰石;用菌体中的脲酶催化可获得100%的方解石。红外光谱分析表明,细胞破碎后的粗脲酶溶液获得的椭圆形碳酸钙受到富含羟基物质的影响。进一步探究了脲酶活性和反应物浓度对球霰石的影响,并发现获得的纯球霰石在7 d内非常稳定,有望作为药物载体使用。

关键词: 脲酶, 酶, 催化, 球霰石, 方解石, 纳米粒子

CLC Number: