CIESC Journal ›› 2016, Vol. 67 ›› Issue (S1): 224-231.doi: 10.11949/j.issn.0438-1157.20151809

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Mass transfer performance for multiple-impeller bioreactor in cold model and fermentation experiments

XIE Minghui1, LU Hongzhong2, TANG Wenjun2, XIA Jianye2, ZHANG Siliang2, ZHOU Guozhong1, YU Peiqing1   

  1. 1 Zhejiang Great Wall Mixers Co., Ltd., Wenzhou 325019, Zhejiang, China;
    2 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2015-12-01 Revised:2015-12-10 Online:2016-08-31 Published:2016-08-31
  • Supported by:

    supported by the Major Program of Zhejiang Province of China (2013C01138) and the Fundamental Research Funds for the Central Universities (222201413037).


Carboxyl methyl cellulose (CMC) solutions with different concentrations simulating different mycelial fermentation broth were used for the study of mass transfer characteristics in bioreactor. Two impeller combinations (triple Rushton turbines, 3PY and bottom hollow blade turbine combined with upper two up-pumping hydrofoils (3 blades), HY+2KCXu) were employed. Under low CMC concentration condition, impeller combination of HY+2KCXu showed better mass transfer capacity. Fermentation of Aspergillus niger with production of glucoamylase was conducted under the two impeller combinations. In order to keep the similar oxygen uptake rate (OUR) among the two combinations, impeller rotation speed of the two configurations was adjusted respectively. Ultimately, based on the investigation of enzyme production, biomass amount, broth rheology and also mass transfer capacity of impellers, conclusions were gotten:apparent viscosity of broth in fermentation with HY+2KCXu was lower than that with 3PY. It seemed that in fermenter equipped with HY+2KCXu pellet prevailed than mycelia, thus lower viscosity was resulted, which in turn increased the mass transfer capacity of the system, that may be the reason why this impeller combination can gave higher product titer.

Key words: impeller combination, non-Newtonian fluid, mass transfer, fermentation, mycelial fungi, bioreactor

CLC Number: 

  • TQ027.2
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