Comparison of three silica resins in chromatographic property for mixed-mode chromatography

doi:10.11949/j.issn.0438-1157.20160095

Abstract

Abstract:

Spherical silica gel with a diameter of 5 μm and pore size of 30 nm was activated by KH-560 and coupled with three kinds of ligands (3-aminopyridine, 2-mercapto benzimidazole and 2-mercapto-1-methylimidazole) to prepare the high performance hydrophobic charge-induction chromatography (HPHCIC) column. The chromatographic properties of three columns were compared and evaluated with three model proteins (Lysozyme, BSA and IgG). The column with 2-mercapto-1-methylimidazole as ligand displayed a proper chromatography and separation behavior which controlled by pH. This work demonstrated that the novel synthetic materials can be utilized as the media of high performance hydrophobic charge induction chromatography.

Key words: bioseparation, protein, high performance liquid chromatography, hydrophobic charge-induction chromatography, retention factor, separation behavior

摘要：

以γ-缩水甘油醚氧丙基三甲氧基硅烷为中间活化剂，将3-氨基吡啶、2-巯基苯并咪唑和2-巯基-1-甲基咪唑3种杂环配基键合到粒径为5 μm、孔径为30 nm的球形硅胶上，制备3种高效疏水电荷诱导色谱填料。利用溶菌酶、牛血清白蛋白和免疫球蛋白G作为模型蛋白，考察其在3种不同配基色谱柱上的保留行为和分离行为。比较了3种不同性质的模型蛋白在不同色谱柱上的色谱性能的异同。发现以2-巯基-1-甲基咪唑为配基的色谱柱具有良好的pH控制色谱行为和良好的分离性能，初步验证了高效疏水电荷诱导色谱的分离模式及潜在应用。

关键词: 生物分离, 蛋白质, 高效液相色谱, 疏水电荷诱导色谱, 保留因子, 分离行为

CLC Number:

TP273

DONG Binbin, XIA Haifeng. Comparison of three silica resins in chromatographic property for mixed-mode chromatography[J]. CIESC Journal, 2016, 67(7): 2893-2900.

董彬彬, 夏海锋. 三种混合模式色谱填料色谱性能的比较[J]. 化工学报, 2016, 67(7): 2893-2900.

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