Controlling strategy for a CANON system treating wastewater with low ammonium concentration at room temperature via bio-augmentation batch enhance method

doi:10.11949/j.issn.0438-1157.20170663

Abstract

Abstract:

The feasibility of the completely autotrophic nitrogen removal over nitrite (CANON) process for removal of nitrogen from mainstream wastewater (~60 mg NH₄⁺-N·L^-1) at room temperature (25℃) was investigated using bio-augmentation batch enhanced (BABE) controlling strategy. The results indicated that through changing 40% sludge of the mainstream reactor from sidestream reactor every 7 d, the total nitrogen removal rate (TNRR) and the total nitrogen removal efficiency (TNRE) stabilized at about 80 g N·m^-3·d^-1 and 70%.The specific anammox activity in mainstream reactor increased continuously. 16S rDNA gene high-throughput sequencing results showed that the functional microorganisms involved in both mainstream and sidestream reactor were Candidatus Jettenia like anammox bacteria and Nitrosomonas like ammonia-oxidizing bacteria (AOB), and the abundance of Nitrospira like nitrite-oxidizing bacteria (NOB) always maintained below 1% during 60 days' operation. It can be concluded that the operational mode of changing sludges between mainstream and sidestream every 7 d guarantees anammox activity and good nitrogen removal performance of mainstream CANON reactor under the experimental conditions herein.

Key words: CANON, bio-augmentation batch enhanced, room temperature and low ammonium concentration, high-throughput sequencing

摘要：

采用侧流富集/主流强化方式，研究了全程自养脱氮工艺（CANON）用于常温（25℃）、低氨氮（约60 mg NH₄⁺-N·L^-1）主流线生物脱氮的可行性。结果表明，通过7 d更换主流反应器40%污泥混合液方式可实现维持主流反应器总氮去除负荷（TNRR）在80 g N·m^-3·d^-1左右，总氮去除率（TNRE）在70%左右，主流反应器厌氧氨氧化比活性持续升高。16S rDNA高通量测序结果表明，主流和侧流CANON系统中起亚硝化作用的氨氧化细菌（AOB）主要是Nitrosomonas属，进行Anammox反应的厌氧氨氧化菌（AnAOB）主要是Candidatus Jettenia属，60 d的运行过程中主流反应器Nitrospira属的亚硝酸盐氧化菌（NOB）丰度始终小于1%。可见在本实验条件下，采用7 d为频率主流和侧流换泥方式，能够保证主流反应器中Anammox活性，确保主流CANON反应器的脱氮性能。

关键词: CANON, 侧流富集/主流强化, 常温低氨氮, 高通量测序

CLC Number:

X703.1

CAO Lijuan, CHEN Jie, JIANG Guangmeng, CHANG Qinglong, WANG Yayi. Controlling strategy for a CANON system treating wastewater with low ammonium concentration at room temperature via bio-augmentation batch enhance method[J]. CIESC Journal, 2017, 68(12): 4723-4730.

曹丽娟, 陈杰, 姜广萌, 常青龙, 王亚宜. 基于侧流富集/主流强化的CANON工艺处理常温低氨氮废水的稳态控制[J]. 化工学报, 2017, 68(12): 4723-4730.

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