Abstract: It is not successful to denitrify waste-water with high content of salt by freshwater activated sludge because of salinity choking.To overcome this problem，halophilic activated sludge must be developed.In this study，activated sediment at estuary of a river was collected and cultured to get halophilic activated sludge.The effect of key factors，such as salinity，temperature，pH and carbon source etc.，on denitrifying-nitrite performance of the halophilic activated sludge was studied.The results show that nitrite can be denitrified by the halophilic system via utilizing it as electron acceptor.As a result，the halophilic activated sludge is of ability to denitrify highly saline wastewater.At 38 g·L-1 salinity environment，the maximum specific denitrification rate of the biological system is 3.29 mg N·（g VSS）-1·h-1.These key operation parameters were optimized for further engineering design：the optimal survival salinity is 15—51 g·L-1 and pH 8.0—9.0.Carbon source has a great influence on denitrification rate.Among methanol，acetate，propionic and glucose tested，methanol is the best because it is beneficial electron donors. As a new different biological system，the halophilic activated sludge，it is of important significance for treatment of saline wastewater to establish their characteristic of denitrification and influencing factors.

Key words: salinity, nitrite denitrifying, halophilic denitrifier, hypersaline wastewater

摘要： 淡水污泥反硝化高盐废水受到盐度抑制而导致处理的失败。为了突破高盐废水脱氮的技术瓶颈，本研究通过采集入海口河底泥发展嗜盐脱氮生物系统实现了高盐废水的脱氮。本文系统地探讨了盐度、温度、pH和碳源类型等关键影响因素对嗜盐污泥反硝化亚硝酸的影响。试验结果表明：采集入海口底泥发展的嗜盐系统可以以亚硝酸盐作为电子受体进行反硝化。在38 g·L-1盐度下，嗜盐反硝化菌以甲醇作为碳源的最大反硝化速率为3.29 mg N·（g VSS）-1·h-1。系统最适宜盐度为15～51 g·L-1，最佳pH范围为8.0～9.0。反硝化碳源类型影响着反硝化速率。在测试的4种碳源类型中，嗜盐反硝化污泥利用甲醇进行反硝化较快。作为新认知的生物系统，确定高盐废水嗜盐生物处理系统的反硝化特性和影响因素对于实现高盐废水的高效处理具有重要的意义。

关键词: 盐度, 反硝化, 嗜盐反硝化菌, 高盐废水