Abstract: Cordyceps ophioglossoides is a valuable traditional medicinal material. We have found that intracellular polysaccharide (IPS) is the major biologically active ingredient in Cordyceps ophioglossoides. This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides. The optimal medium for IPS production consists of glucose 54.50 g•L?1, yeast powder 25.50 g•L?1, NaH2PO4 0.4 g•L?1 and K2HPO4 0.4 g•L?1. The suggested culture conditions are 24 °C, initial pH 4.5 with a rotary speed of 120 r•min?1 for 168 h. The yield of IPS is 737.93 mg•L?1, which is 50% higher than the yield under the conditions prior to optimization. The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay. The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

Key words: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization

摘要： Cordyceps ophioglossoides is a valuable traditional medicinal material. We have found that intracellular polysaccharide (IPS) is the major biologically active ingredient in Cordyceps ophioglossoides. This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides. The optimal medium for IPS production consists of glucose 54.50 g•L?1, yeast powder 25.50 g•L?1, NaH2PO4 0.4 g•L?1 and K2HPO4 0.4 g•L?1. The suggested culture conditions are 24 °C, initial pH 4.5 with a rotary speed of 120 r•min?1 for 168 h. The yield of IPS is 737.93 mg•L?1, which is 50% higher than the yield under the conditions prior to optimization. The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay. The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

关键词: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization