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LI Jiayou; CAO Yu; LIU Yi; QIAN Shaosong; JIAO Qingcai
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李加友; 曹瑜; 刘毅; 钱绍松; 焦庆才
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Abstract: A novel Enterococcus faecalis strain designated N J402 was found with high activity of arginine deiminase (ADI). The optimum condition for catalytic activity was determined in terms of temperature (about 40℃), thermostability (available 37℃) and pH (6-7). The effects of substrate and product concentration were studied. The effects of various metal ions added in reaction mixtures on the biocatalyst were investigated and ADI of N J402 was found to exhibit Co^2+ dependence, different from previous reports. Surfactant, cetyl trimethyl ammonium bromide, was one of the most important keys for producing L-citrulline. The enzyme in resting cells possessed the quality of high stability for reuse.
Key words: L-citrulline, L-arginine, arginine deiminase, activity, optimization
摘要: A novel Enterococcus faecalis strain designated N J402 was found with high activity of arginine deiminase (ADI). The optimum condition for catalytic activity was determined in terms of temperature (about 40℃), thermostability (available 37℃) and pH (6-7). The effects of substrate and product concentration were studied. The effects of various metal ions added in reaction mixtures on the biocatalyst were investigated and ADI of N J402 was found to exhibit Co^2+ dependence, different from previous reports. Surfactant, cetyl trimethyl ammonium bromide, was one of the most important keys for producing L-citrulline. The enzyme in resting cells possessed the quality of high stability for reuse.
关键词: L-瓜氨酸酶;制备方法;精氨酸脱亚氨酸酶;活性;稳定性
LI Jiayou, CAO Yu, LIU Yi, QIAN Shaosong, JIAO Qingcai. Activity and Stability of Arginine Deiminase for Producing L-citrulline[J]. .
李加友,曹瑜,刘毅,钱绍松,焦庆才. L-瓜氨酸酶法制备中精氨酸脱亚氨酸酶的活性与稳定性研究[J]. CIESC Journal.
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https://hgxb.cip.com.cn/EN/Y2005/V13/I6/841