化工学报 ›› 2013, Vol. 64 ›› Issue (1): 233-242.DOI: 10.3969/j.issn.0438-1157.2013.01.025

• 综述与专论 • 上一篇    下一篇

核酸适配体在生化分离及检测领域中的研究进展

李晓佩1,2, 杨良嵘1, 黄昆1, 李文松1,2, 孙西同1,2, 刘会洲1   

  1. 1. 中国科学院过程工程研究所,中国科学院绿色过程与工程重点实验室,北京 100190;
    2. 中国科学院研究生院,北京 100190
  • 收稿日期:2012-04-25 修回日期:2012-08-02 出版日期:2013-01-05 发布日期:2013-01-05
  • 通讯作者: 杨良嵘,刘会洲
  • 作者简介:李晓佩(1984—),女,博士研究生。
  • 基金资助:

    国家自然科学基金项目(21106162);国家自然科学重点基金项目(21136009);国家重点基础研究发展计划项目(2009CB219904);化学工程国家重点实验室开放基金项目(SKL-ChE-11A04)。

Research progress of aptamer in biochemical separation and detection

LI Xiaopei1,2, YANG Liangrong1, HUANG Kun1, LI Wensong1,2, SUN Xitong1,2, LIU Huizhou1   

  1. 1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    2. Graduate School of Chinese Academy of Sciences, Beijing 100190, China
  • Received:2012-04-25 Revised:2012-08-02 Online:2013-01-05 Published:2013-01-05
  • Supported by:

    supported by the National Natural Science Foundation of China (21106162, 21136009),the National Basic Research Program of China (2009CB219904) and the Chemical Engineering, State Key Laboratory Foundation (SKL-ChE-11A04).

摘要: 核酸适配体是一种能够特异性地识别目标物的寡聚核苷酸,可以是RNA也可以是DNA。较其他识别分子而言,适配体具有性质稳定、易合成、易标记、分子量较小和目标分子广泛等优势。目前核酸适配体主要被应用在检测、分离纯化和医疗三大领域,本文主要论述了适配体在生化分离及检测领域中的研究进展。

关键词: 核酸适配体, 识别分子, 生化分离, 检测

Abstract: Aptamers are short single stranded DNA or RNA oligonucleotides that exhibit high affinity and specificity to their corresponding targets.Compared to other recognition molecules, aptamers are highly stable, easier to synthesize and modify, smaller in size and specific to a wide range of targets.Until now, aptamers have shown diverse applications to detection, separation and purification studies, and development of medical treatment.This paper summarizes the research progress of aptamer in biochemical separation and detection.

Key words: aptamers, recognition-molecules, biochemical separation, detection

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