化工学报 ›› 2010, Vol. 61 ›› Issue (10): 2667-2672.

• 生物化学工程与技术 • 上一篇    下一篇

热水预抽提后竹子的纤维素酶解

吕健; 詹怀宇;胡会超;付时雨   

  1. 华南理工大学制浆造纸工程国家重点实验室
  • 出版日期:2010-10-05 发布日期:2010-10-05

Hydrolysis of bamboo pre-extracted with hot water by enzyme

LV Jian;ZHAN Huaiyu;HU Huichao;FU Shiyu   

  • Online:2010-10-05 Published:2010-10-05

摘要:

研究了热水预抽提后竹子的纤维素酶水解过程。经过热水预抽提的竹子,由于其结构较疏松,酶解生成葡萄糖的效率显著提高。当热水抽提H因子为1000和2000,酶解反应时间为24 h时,生成的葡萄糖含量较未抽提的竹子分别提高了43%和215%,热水预抽提最优的H因子为2000。随着初始酶用量的增加,酶解生成的葡萄糖的含量也随之增加,但增加量并不与初始酶的增加量呈比例。双曲线模型可用于模拟不同初始酶用量、不同酶解时间下葡萄糖的生成规律。

关键词:

竹子, 纤维素酶, 水解, 葡萄糖, 预抽提

Abstract:

The hydrolysis process of bamboo pre-extracted with hot water by enzyme was studied.It was found that the efficiency of hydrolysis to glucose by enzyme was much higher for pre-extracted bamboo than for original one, because its structure was loosened up.The glucose formed increased by about 43% and 215%, respectively, when the H factor in pre-extraction with hot water was 1000 and 2000, and enzymolysis time 24 h.The optimum H factor was 2000.The content of glucose formed was increase with initial concentration of enzyme used, but not be proportional.The process that is the effect of initial concentration of enzyme and enzymolysis time on glucose formation can be simulated by a hyperbolic model.

Key words:

竹子, 纤维素酶, 水解, 葡萄糖, 预抽提