甘蔗渣在高温液态水中的超微结构与组分变化

doi:10.3969/j.issn.0438-1157.2014.12.049

化工学报 ›› 2014, Vol. 65 ›› Issue (12): 5010-5016.DOI: 10.3969/j.issn.0438-1157.2014.12.049

甘蔗渣在高温液态水中的超微结构与组分变化

余强, 庄新姝, 袁振宏, 孔晓英, 亓伟, 王闻, 王琼, 谭雪松

中国科学院广州能源研究所, 中国科学院可再生能源重点实验室, 广东广州 510640

收稿日期:2014-05-23 修回日期:2014-07-23 出版日期:2014-12-05 发布日期:2014-12-05
通讯作者: 袁振宏
基金资助:
国家自然科学基金项目(21206163);江苏省生物质绿色燃料与化学品重点实验室开放基金面上项目(JSBGFC12007);广东省自然科学基金项目(S2012040007407);国家重点基础研究发展计划项目(2012CB215304);国家高技术研究发展计划项目(2012AA101802).

Change of ultrastructure and composition of sugarcane bagasse in liquid hot water

YU Qiang, ZHUANG Xinshu, YUAN Zhenhong, KONG Xiaoying, QI Wei, WANG Wen, WANG Qiong, TAN Xuesong

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China

Received:2014-05-23 Revised:2014-07-23 Online:2014-12-05 Published:2014-12-05
Supported by:
supported by the National Natural Science Foundation of China (21206163), Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals (JSBGFC12007), the Natural Science Foundation of Guangdong Province (S2012040007407), the National Basic Research Program of China (2012CB215304) and the National High Technology Research and Development Program of China(2012AA101802).

摘要/Abstract

摘要： 木质纤维素类物质中天然纤维素与半纤维素、木质素等组分交联形成了坚固的细胞壁,对纤维素酶水解和微生物消化表现出一定的抗性,原料预处理可以克服细胞壁抗性,提高木质纤维多糖生化转化效率.从细胞壁超微结构层次入手,对甘蔗渣细胞壁在高温液态水预处理过程中的解构机理进行了深入研究.未处理甘蔗渣细胞壁分层现象明显,由外至内分别为胞间层(ML)、初生壁(P)及次生壁(S),高温液态水预处理后各层界线变得模糊.SEM-EDXA分析表明细胞壁各层木质素分布发生了迁移,水解液中的木聚糖和木质素衍生物在细胞壁表面凝集生成类木质素滴状沉淀物.拉曼光谱分析结果显示预处理后纤维素在细胞壁各层分布趋于均质化.

关键词: 高温液态水, 生物质, 显微结构, 化学分析

Abstract: Native lignocellulosic biomass has limited accessibility to enzymes and microorganisms due to its complex cell wall structure of cellulose-hemicellulose-lignin. Therefore, pretreatment is a prerequisite to overcome recalcitrance of biomass and enhance bio-chemical conversion ratio of polysaccharides. Compared with other methods, high temperature liquid hot water pretreatment has the advantages of no chemical addition and less inhibitory products. In the present study, different structural changes at plant tissue, cellular, and cell wall levels were investigated to understand the decomposition mechanism of sugarcane bagasse cell wall in the liquid hot water pretreatment. Transmission electron microscopy (TEM) images showed that sugarcane bagasse cell walls were composed of middle lamella (ML) layers, primary wall (P) layers, and secondary wall layers (S). While after the pretreatment, the boundaries among the ML, P and S layers of treated samples could not be distinguished exactly. The data from scanning electron microscopy and energy dispersive X-ray analysis(SEM-EDXA)showed that migration of lignin happened among different cell wall layers. Moreover, pseudo-lignin, the degradation products of lignin and xylan, appeared on the surface of pretreated sugarcane bagasse. Furthermore, Raman spectra of treated sugarcane bagasse indicated that distribution of cellulose in the cell wall was homogenized, and the difference in chemical composition was reduced.

Key words: liquid hot water, biomass, microstructure, chemical analysis

中图分类号:

TQ353

余强, 庄新姝, 袁振宏, 孔晓英, 亓伟, 王闻, 王琼, 谭雪松. 甘蔗渣在高温液态水中的超微结构与组分变化[J]. 化工学报, 2014, 65(12): 5010-5016.

YU Qiang, ZHUANG Xinshu, YUAN Zhenhong, KONG Xiaoying, QI Wei, WANG Wen, WANG Qiong, TAN Xuesong. Change of ultrastructure and composition of sugarcane bagasse in liquid hot water[J]. CIESC Journal, 2014, 65(12): 5010-5016.

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甘蔗渣在高温液态水中的超微结构与组分变化

Change of ultrastructure and composition of sugarcane bagasse in liquid hot water

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