化工学报 ›› 2020, Vol. 71 ›› Issue (10): 4711-4719.DOI: 10.11949/0438-1157.20200346

• 分离工程 • 上一篇    下一篇

磁性温敏分子印迹材料用于药用植物中富集单一成分——芒柄花黄素的分离与富集

何家垣1(),蒋壮飞1,马蓉蓉1,杨莉莉1,李清瑶1,谭玲2,陈志涛1,张起辉1()   

  1. 1.重庆大学化学化工学院,重庆 400044
    2.重庆大学药学院,重庆 400044
  • 收稿日期:2020-04-01 修回日期:2020-05-22 出版日期:2020-10-05 发布日期:2020-10-05
  • 通讯作者: 张起辉
  • 作者简介:何家垣(1995-),男,硕士研究生,201818021132@cqu.edu.cn
  • 基金资助:
    国家自然科学基金项目(819734512);医工联合项目(2019CDYGYB027);2019技术预见与制度创新项目(cstc2019jsyj-yzysbAX00204);重庆市科委项目(cstc2018jcyjAX06615);重庆市教育委员会科学技术研究项目(KJZD-K2018001036);重庆市研究生创新基金项目(CYS180337);中央高校基本科研业务费(2019CDXYHG00131)

Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants

Jiayuan HE1(),Zhuangfei JIANG1,Rongrong MA1,Lili YANG1,Qingyao LI1,Ling TAN2,Zhitao CHEN1,Qihui ZHANG1()   

  1. 1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
    2.School of Pharmacy, Chongqing University, Chongqing 400044, China
  • Received:2020-04-01 Revised:2020-05-22 Online:2020-10-05 Published:2020-10-05
  • Contact: Qihui ZHANG

摘要:

以四氧化三铁(Fe3O4)纳米粒子为支撑材料,模板选用芒柄花黄素,温敏功能单体和辅助功能单体分别选用N-异丙基丙烯酰胺(NIPAM)和甲基丙烯酸(MAA),制备出磁性和温度双响应型分子印迹材料(MTMIPs),并结合高效液相色谱法(HPLC)将其用于红车轴草中芒柄花黄素的定向萃取研究。利用扫描电镜(SEM)、透射电镜(TEM)、红外光谱(FT-IR)、热重分析(TGA)、磁性分析(VSM)等手段对MTMIPs进行表征,随后对其温敏及吸附性能进行考察。结果表明,MTMIPs为核壳型结构,热稳定性好,具有良好的吸附性能(16.43 mg/g)和温敏特性,对芒柄花黄素的吸附动力学符合准二级动力学模型,吸附过程符合Langmuir单分子层吸附。HPLC检测结果显示MTMIPs可用于从复杂样品中分离富集芒柄花黄素。

关键词: 磁性温敏材料, 分子印迹材料, 聚合物, 吸附剂, 纳米材料

Abstract:

The magnetic and temperature double response molecularly imprinted polymers for formononetin-specific adsorption (MTMIPs) were successfully prepared by using Fe3O4 as supporting matrix, formononetin as template, N-isopropyl acrylamide as the thermo-sensitive type functional monomer and methacrylic acid as auxiliary functional monomer. SEM, TEM, FT-IR, TGA and magnetic analysis were used to characterize the structure of MTMIPs, and then their adsorption properties and reproducibility were investigated. The results showed that MTMIPs was a core-shell structure with good thermal stability, good adsorption performance (16.43 mg/g) and fast adsorption performance. The adsorption kinetics of formononetin was consistent with the quasi-second-order kinetics model, and the adsorption process was consistent with the Langmuir monolayer adsorption with good reproducibility. HPLC test results show that MTMIPs can be used to separate and enrich formononetin from complex samples.

Key words: magnetic temperature-sensitive materials, molecular imprinted materials, polymers, adsorbents, nanomaterials

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