纳米结构酶催化剂研究进展

doi:10.11949/0438-1157.20211409

摘要/Abstract

摘要：

将酶负载于载体上可以提高工业应用中酶的稳定性并便于重复使用。纳米材料的发展为固定化酶提供了新的契机，利用纳米材料固定化酶有望进一步提高酶在工业环境中的催化性能、拓展固定化酶的应用范围。本文主要关注近年来在纳米结构酶催化剂方面的研究进展，重点介绍本研究组以无机晶体、金属有机骨架材料、石墨烯和功能高分子等为载体进行酶固定化以及酶催化过程多尺度分子模拟方法的研究进展，讨论了纳米结构酶催化剂的发展前景。

关键词: 固定化酶, 纳米材料, 生物催化, 酶催化

Abstract:

Loading the enzyme on the carrier can improve the stability of the enzyme in industrial applications and facilitate repeated use. Utilizing nanostructured materials for enzyme immobilization has offered unprecedented opportunities in enhancing enzyme stability in adverse conditions found in industrial practices and thus extending the application spectra of enzymatic catalysis. This review summarizes recent progress of our group in exploring nanostructured enzyme catalysts using inorganic crystals, metal-organic frameworks, graphene and environmentally responsive polymers as matrixes, as well as a new molecular simulation method that combines with Markov-state modeling for establishing a process profile with molecular insight. The prospects of the nanostructured enzyme catalysts are also discussed.

Key words: enzyme immobilization, nanomaterials, biocatalysis, enzymatic catalysis

中图分类号:

TQ 028.8

夏欢, 卢滇楠, 戈钧, 吴建中, 刘铮. 纳米结构酶催化剂研究进展[J]. 化工学报, 2021, 72(12): 6086-6092.

Huan XIA, Diannan LU, Jun GE, Jianzhong WU, Zheng LIU. Advances in nanostructured enzyme catalysts[J]. CIESC Journal, 2021, 72(12): 6086-6092.

图/表 3

图1 纳米结构酶催化剂的研究进展

Fig.1 The research progress of nanostructured enzyme catalysts

图2 采用原位合成法制备细胞色素C-ZIF-8复合物[46]

Fig.2 Preparation of Cytochrome C-ZIF-8 composites by an insitu approach[46]

图3 脂肪酶-氧化石墨烯气凝胶复合物的制备过程(a)，气态酶催化实验装置示意图(b)，连续操作下脂肪酶-氧化石墨烯气凝胶的活性变化图(c)[50]

Fig.3 Preparation of procedures of lipase-GO aerogel (a), schematic diagram of experimental setup for gaseous enzymatic catalysis (b), the activity of lipase-GO aerogel during a continuous operation for 500 h (c)[50]

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