关键词: 脱细胞真皮基质, 微结构, 碱液, 细胞相容性

Abstract:

Acellular dermal matrix (ADM）has been widely used as a dermal scaffold material in tissue engineering, while its porosity is low and pore interconnectivity is poor.The microstructure is not ideal enough for cell attachment and viability.In light of this, a porcine ADM was prepared by combined treatments with enzyme, alkali, sodium dodecyl sulfonate and sodium chloride solution for forming a desired microstructure.The relationship between the microstructure of the ADM and treatments with various alkali solutions was analyzed by using scanning electron microscopy (SEM).Mouse embryonic fibroblasts (3T3）were cultured on the ADM and cell proliferation tests were performed by colorimetric MTT assay.The ADM stained with Rhodamine 110 and cells stained with acridine orange were viewed under a confocal laser scanning microscope (CLSM).SEM images displayed that the ADM made up of natural collagen had three-dimensional interconnected channels with diameter of about 100 μm and no cell was observed.The alkali concentrations affect the pore structure and morphology of the ADM.The MTT assay indicates that the cells cultured on the ADM proliferated well and CLSM image showed even distribution of cells.The prepared ADM had good microstructure and cytocompatibility.It is expected to have potential application in tissue engineering.

Key words: 脱细胞真皮基质, 微结构, 碱液, 细胞相容性