CIESC Journal ›› 2019, Vol. 70 ›› Issue (9): 3213-3227.DOI: 10.11949/0438-1157.20190328

• Reviews and monographs • Previous Articles     Next Articles

Progress and application of collagenase research

Yihang SONG1(),Chuhao WANG1,Baishan FANG1,2,3()   

  1. 1. College of Chemistry and Chemical Engineering, Xiamen University
    2. The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University
    3. The Key Laboratory for Chemical Biology of Fujian Province, Xiamen 361000, Fujian, China
  • Received:2019-04-01 Revised:2019-05-17 Online:2019-09-05 Published:2019-09-05
  • Contact: Baishan FANG

胶原酶研究进展与应用

宋易航1(),王楚浩1,方柏山1,2,3()   

  1. 1. 厦门大学化学化工学院
    2. 厦门市合成生物学重点实验室
    3. 福建省化学生物学重点实验室,福建 厦门 361000
  • 通讯作者: 方柏山
  • 作者简介:宋易航(1993—),男,硕士研究生,tjusyh@foxmail.com
  • 基金资助:
    国家自然科学基金项目(21336009)

Abstract:

Collagen is widely distributed in various tissues of living organisms and is one of the basic proteins that constitute living organisms. The structure of collagen is more complicated than that of general protein and it displays a more stable property so that normal proteases have no significant effect to deal with it. There are only two typical enzymes have ability to hydrolyze collagen in situ, one is called matrix metalloproteinase (MMP) and another is bacterial collagenase. In this review the collagen and hydrolase are introduced in briefly, then the development process and the hydrolysis mechanism to collagen between matrix metalloproteinase and collagenase are presented, then the particular aspects of the different enzyme activities will be contextualized within relevant areas of application, mainly about therapeutics, food processing, environmental protection and tissue engineering. In the end the development of the present research and guide further potential research orientation of the system of collagen & collagenase is summarized.

Key words: bioengineering, enzyme, protein, matrix metalloproteinase, bacterial collagenase

摘要:

胶原蛋白广泛存在于有机体生命各组织,是构成生命体的基础性蛋白之一。胶原蛋白结构相对复杂、稳定性良好、不易受到普通蛋白酶的降解破坏。而基质金属蛋白酶和微生物胶原酶是为数不多能原位降解胶原蛋白的两类胶原酶,能够在生理环境下保持较高酶活力从而发挥作用,因此受到研究者的广泛关注。首先简要介绍胶原蛋白及其水解酶,其次重点介绍基质金属蛋白酶和微生物胶原酶的发掘历程和水解机理,然后综述这两类酶的最新研究进展及其在医疗诊治、食品加工、环境保护及组织工程等多方面的应用,最后总结胶原酶及胶原蛋白研究现状、研究难点和未来研究方向。

关键词: 生物工程, 酶, 蛋白质, 基质金属蛋白酶, 微生物胶原酶

CLC Number: