Abstract:

Immobilized Anammox granules were used as sequential biocatalyst addition to substitute particulate sludge for starting up Anammox reactor, and the kinetic characteristics of the immobilized granules were investigated through batch tests. The reactor could be successfully started up in 49 d with immobilized Anammox granules as sequential biocatalyst addition, and the removal rates of NH⁺₄-N and NO^-₂-N were 80.7% and 83.1%, respectively. Kinetics study showed that the half saturation constants for ammonium and nitrite of the immobilized granules were 1.57 mmol·L^-1 and 1.505 mmol·L^-1, the half inhibition constants of ammonium and nitrite were 724.2 mmol·L^-1 and 66.65 mmol·L^-1, respectively. The immobilized granules were easier to gain substrate in adverse environment and could suffer higher substrate concentration. The better kinetic performance of the immobilized Anammox granules contributed to the successful biomass supplementation performance, which was helpful for the novel biomass supplementation process for enhancement of Anammox performance.

Key words: anaerobic ammonium oxidation, immobilized granules, immobilization, start-up, kinetics

摘要：

以厌氧氨氧化菌包埋颗粒代替颗粒污泥作为流加菌启动厌氧氨氧化反应器,并采用批次试验研究了包埋颗粒的动力学特性。结果表明,以包埋颗粒作为流加菌,在49 d内成功实现了厌氧氨氧化反应器的加速启动,反应器对NH⁺₄-N和NO^-₂-N的去除率分别为80.7%和83.1%,总氮去除负荷为0.505 kg·m^-3·d^-1。动力学研究表明,包埋颗粒对氨氮和亚硝氮的半速率常数分别为1.57 mmol·L^-1和1.505 mmol·L^-1。包埋颗粒对氨和亚硝酸的抑制常数分别为724.2 mmol·L^-1和66.65 mmol·L^-1。厌氧氨氧化菌包埋颗粒具有优良的动力学特性,作为流加菌效果显著,对新型厌氧氨氧化菌种流加技术的发展具有积极的意义。

关键词: 厌氧氨氧化, 包埋菌, 固定化, 启动, 动力学