Abstract: The variation of electron transport system（ETS）activity during organic matter biodegradation and nitrification was studied to evaluate the feasibility of assessment of biological activity of activated sludge by ETS activity. The Michaelis - Menten equation was used to analyze the kinetics of organic matter oxidation and nitrification. The experimental result showed that ETS activity could reflect the reaction course of organic matter biodegradation and nitrification, and the changes in loading and alkalinity. Therefore, ETS activity could be used as a parameter for assessing the biological activity of activated sludge. The Michaelis constants were K_s^T=368.9 mg·L^-1, U_m^T=90.9 mg TF·(g TSS·h)^-1, K_s^I=88.42 mg·L^-1, U_m^I=277.8 mg INTF·(g TSS·h)^-1 during organic matter biodegradation. The Michaelis constants were K_s^T=16.89 mg·L^-1,U_m^T=34.6 mg TF·(g TSS·h)^-1, K_s^T=6.0 mg·L^-1, U_m^I=196.08 mg INTF·(g TSS·h)^-1 during nitrification. The results further showed that heterotrophic bacteria growth rate in biodegradation of organic matter was higher than autotrophic denitrifying bacteria growth rate in nitrification of organic matter.

Key words: organic matter biodegradation, nitrification, ETS activity, activated sludge, kinetics

摘要： 通过考察有机物生物降解和氨氮生物硝化过程中活性污泥电子传递体系（ETS）活性的变化规律，研究了ETS活性表征污泥生物活性的可行性，结合米门公式分析了有机物生物降解和硝化反应过程生物活性动力学。试验结果表明，活性污泥的ETS活性可以有效地揭示出有机物生物降解和氨氮生物硝化反应的进程，同时对系统受到的有机物和氨氮冲击负荷及硝化过程中碱度的变化有着灵敏的反映，这说明用ETS活性表征污泥的生物活性是可行的；有机物生物降解过程生物活性米氏常数K_s^T=368.9 mg·L^-1，U_m^T=90.9 mg TF·（g TSS·h）^-1，K_s^I=88.42 mg·L^-1，U_m^I=277.8 mg INTF·（g TSS·h）^-1；氨氮硝化过程生物活性米氏常数K_s^T=16.89 mg·L^-1，U_m^T=34.6 mg TF·（g TSS·h）^-1， K_s^I=6.0 mg·L^-1，U_m^I=196.08 mg INTF·（g TSS·h）^-1；生物活性动力学分析进一步验证了进行有机物生物降解的异养菌生长速率高于进行硝化反应的自养型硝化菌。

关键词: 有机物降解, 硝化反应, ETS活性, 活性污泥, 动力学