Electrochemistry of glucose oxidase on modified carbon nanotubes

doi:10.3969/j.issn.0438-1157.2014.05.030

Abstract

Abstract: Glucose oxidase (GOx) was immobilized on the electrode surface of multi-walled carbon nanotubes, amino functionalized carbon nanotubes (AMWNTs) and carboxyl functionalized carbon nanotubes (MWNTs-COOH). Electrochemical measurements indicated that the formal potentials of GOx immobilized on AMWNTs and MWNTs-COOH did not change, but their peak currents were improved. The peak current of GOx immobilized on AMWNTs was four times larger than that immobilized on MWNTs. The electrochemistry behavior of Nafion/GOx-AMWNTs/GC electrode were further characterized. The results indicated that GOx immobilized on AMWNTs could undergo a direct quasi-reversible electrochemical reaction and show good stability. Amino-functionalized electrodes could significantly improve the performance of GOx-based biofuel cells.

Key words: enzyme, immobilization, electrochemistry, protein, adsorption, biofuel cell

摘要： 将葡萄糖氧化酶（GOx）分别固定在多壁碳纳米管（MWNT）、氨基化碳纳米管（AMWNTs）和羧基化碳纳米管（MWNTs-COOH）修饰的电极表面，电化学测量表明固定在羧基和氨基碳纳米管上的GOx式量电位基本没变，而峰电流得到了很大提高。尤其是氨基化碳纳米管上的GOx的峰电流是未功能化碳管上GOx的4倍多。进一步研究Nafion/GOx-AMWNTs/GC电极的电化学行为，发现固定在AMWNTs上的GOx可进行直接准可逆的氧化还原反应，而且固定在AMWNTs上的GOx有良好的稳定性。氨基改性碳纳米管电极载体材料有望显著提高GOx生物燃料电池性能。

关键词: 酶, 固定化, 电化学, 蛋白质, 吸附, 生物燃料电池

CLC Number:

O646

WANG Jia, LI Junhua, ZHOU Jian. Electrochemistry of glucose oxidase on modified carbon nanotubes[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.030.

王佳, 李俊华, 周健. 葡萄糖氧化酶在修饰碳纳米管上的电化学[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.030.

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