Abstract:

Using C18 as the stationary phase and methanol/water=70/30 (volume ratio) as the mobile phase, the three-zone simulated moving bed was used to separate two alkaloids, evodiamine and rutaecarpine. The isotherms of the two alkaloids were determined by frontal analysis, which can be described by linear isotherms with the Henry coefficients of 3.11 for evodiamine and 5.25 for rutaecarpine. The axial diffusion coefficient and mass-transfer coefficients of the two alkaloids were estimated by empirical equations. The operation conditions were designed by triangle theory and optimization method based on the mathematical model. Through optimization, the maximum feed flow rate was 0.55 ml?min^-1. The experimental purities of two products were both higher than 99%. By asynchronous switching, the feed flow rate was increased to 0.62 ml?min^-1 without additional investment and product purity loss.

Key words: simulated moving bed, chromatography, separation, evodiamine, rutaecarpine, Varicol, optimization

摘要：

以C18为固定相、甲醇/水=70/30（体积比）为流动相，利用三区带模拟移动床分离了两种生物碱，吴茱萸碱和吴茱萸次碱。通过前沿分析法测定了两种生物碱的吸附等温线，在实验浓度范围内符合线性吸附等温线，吴茱萸碱和吴茱萸次碱的亨利系数分别为3.11和5.25。利用经验公式估算了轴向扩散系数和有效传质系数。分别利用三角形理论和基于模型的优化方法对三区带模拟移动床的操作条件进行设计，在优化的条件下，最大进料流量为0.55 ml?min^-1，两种产品纯度均大于99%。通过异步切换策略，在不增加设备投资及保证产品纯度大于99%的前提下，将进料流量提高至0.62 ml?min^-1。

关键词: 模拟移动床, 色谱, 分离, 吴茱萸碱, 吴茱萸次碱, Varicol, 优化