化工学报

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金属纳米颗粒辅助木质纤维素暗发酵生物制氢的研究进展

童海航(),石德智(),刘嘉宇,蔡桦伊,罗丹,陈飞   

  1. 重庆大学环境与生态学院 三峡库区生态环境教育部重点实验室,重庆 400045
  • 收稿日期:2021-10-08 修回日期:2022-01-08
  • 通讯作者: 石德智
  • 作者简介:童海航(1998—),男,硕士研究生,1046902611@qq.com
  • 基金资助:
    国家自然科学基金项目(51908528);中央高校基本科研业务费专项(2021CDHQY-014);重庆大学高层次人才启动项目(0219001104444)

Research progress on dark fermentative bio-hydrogen production from lignocellulose assisted by metal nanoparticles

Haihang TONG(),Dezhi SHI(),Jiayu LIU,Huayi CAI,Dan LUO,Fei CHEN   

  1. College of Environment and Ecology, Key Laboratory of the Three Gorges Reservoir Region ' s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China
  • Received:2021-10-08 Revised:2022-01-08
  • Contact: Dezhi SHI

摘要:

通过文献计量学分析表明暗发酵制氢是目前研究最热门的生物制氢方法,Fe、Ni、Co、Ag等金属纳米颗粒作为该领域研究热点可改善暗发酵制氢存在底物转化率与产氢效率均有待提高的难题。介绍了金属纳米颗粒的特点、生物相容性及其与酶、微生物细胞的作用机理,进一步从促进木质纤维素水解影响产氢、对水解酶的固定化影响产氢、提高氢化酶活性影响产氢、调控发酵微生物细胞代谢和促进细胞电子传递影响产氢、改善微生物群落结构影响多菌群协同产氢等几个方面对典型金属纳米颗粒辅助木质纤维素暗发酵产氢的研究现状进行综述,并对金属纳米颗粒应用于暗发酵产氢存在的难点及前景方向进行了展望。

关键词: 木质纤维素, 暗发酵, 生物制氢, 金属纳米颗粒, 酶, 微生物

Abstract:

Based on bibliometric analysis of bio-hydrogen published 2004 to 2020 indexed by web of science, dark fermentation as one of the four methods of bio-hydrogen, has received the highest attention for its assets such as high hydrogen-production rate without light, low energy consumption and comprehensively available microorganism and substrate. At the same time, some metal nanoparticles (Fe, Ni, Co, and Ag) that exhibit excellent promotion for dark fermentation has been spotlight in this research field. Nanoparticles equipped with the properties and characteristics of small volume, surface effect and quantum size effect can enhance the rate of electron transfer of cells and present good biocompatibility. The mechanism of metal nanoparticles (MNPs) interacting with enzymes and microoganisms has been illustrated. Particularly, impacts of MNPs are elaborated in details on different mechanisms of enhancing the hydrogen production process through dark fermentation by promoting hydrolysis of lignocellulose, immobilization of cellulase enzymes, and activity of hydrogenase, respectively. On one hand, MNPs assist pretreatment process of removing of lignin, and improve stability of enzymes by immobilizing them; on the other hand, MNPs enhance the rate of electron transfer, thus make a promotion of activity of hydrogenase, and finally lead the increase of hydrogen production. In addition to the impact of nanoparticles on enzymes, the role of nanoparticles on bacterial metabolism, interspecies electron transfer and synergistic hydrogen production of mixed culture are discussed. The difficulties and prospects of the application of metal nanoparticles in dark fermentation for hydrogen production are also prospected.

Key words: lignocellulose, dark fermentative, bio-hydrogen, metal nanoparticles, enzyme, microorganism

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