关键词: 生物能源, 电化学厌氧消化, 微生物群落, 代谢, 酶

Abstract:

In order to explore the effect of voltage perturbation on the activities of microorganisms and key enzymes in the metabolic flux of the electrochemical anaerobic digestion(EAD), a single-chamber EAD reactor was used in the experiment, the electrolysis voltage was used as the perturbation variable, and a metabolic model was constructed by using CNA. Quantitative changes are discussed. The results showed that after the voltage disturbance, the methanogenesis pathway was biased towards hydrogenotrophic methanogenesis, accounting for about 38.5%, and the 0.6 V disturbance showed the best methane flux of 0.5222 g. The main sources of H₂ in the EAD system are the cathode and NADH, and the perturbation of the electrolysis voltage will affect the production of H₂, which in turn will affect the methane production flux. After 0.6 V perturbation, the relative abundance of Trichloromonas in the biofilm was as high as 40.2%, which was 1.52 times and 1.13 times that of 1.0 V and 1.4 V, respectively. Meanwhile, CoI, PTA, AK and CoF₄₂₀ all showed the best enzyme activity levels. The activity levels of key enzymes and the relative abundance of Trichloromonas showed good consistency with the methanogenesis flux, and the microbial diversity in the anode biofilm was also an important factor affecting the methanogenesis flux in EAD.

Key words: bioenergy, electrochemical anaerobic digestion, microbial communities, metabolism, enzyme