甲烷化抑制剂在微生物电化学合成乙酸系统中的生物抑制效应

doi:10.11949/j.issn.0438-1157.20151517

化工学报 ›› 2016, Vol. 67 ›› Issue (5): 2033-2040.DOI: 10.11949/j.issn.0438-1157.20151517

甲烷化抑制剂在微生物电化学合成乙酸系统中的生物抑制效应

戚玉娇^1,2, BRIDIER Arnaud², DESMOND LE QUEMENER Elie², 吕凡^1,3, 何品晶^1,3, BOUCHEZ Théodore²

1 同济大学固体废物处理与资源化研究所, 上海 200092;
2 法国农业与环境科技研究所(IRSTEA), 巴黎 92761;
3 住房与城乡建设部村镇建设司农村生活垃圾处理技术研究与培训中心, 上海 200092

收稿日期:2015-10-08 修回日期:2015-11-26 出版日期:2016-05-05 发布日期:2016-05-05
通讯作者: BOUCHEZ Théodore, 何品晶
基金资助:
法国国家科研署基金项目(BIORARE,ANR-10-BTBR-02);国家自然科学基金项目(21177096,51378375);111引智项目。

Selective inhibition of methanogens using 2-bromoethanesulfonate for improvement of acetate production from CO₂ in bioelectrochemical systems

QI Yujiao^1,2, BRIDIER Arnaud², DESMOND LE QUEMENER Elie², LÜ Fan^1,3, HE Pinjing^1,3, BOUCHEZ Théodore²

1 Institute of Waste Treatment and Reclamation, Tongji University, Shanghai 200092, China;
2 Irstea, UR HBAN, 1 rue Pierre-Gilles de Gennes CS10030, 92761 Antony cedex, France;
3 Centre for the Technology Research and Training on Household Waste in Small Towns & Rural Area, Ministry of Housing and Urban-Rural Development of PR. China (MOHURD), Shanghai 200092, China

Received:2015-10-08 Revised:2015-11-26 Online:2016-05-05 Published:2016-05-05
Supported by:
supported by the French Investissement d'Avenir Program (ANR-10-BTBR-02), the National Natural Science Foundation of China (21177096, 51378375), and the 111 Program.

摘要/Abstract

摘要：

研究了利用2-溴乙烷磺酸钠(BES)选择性抑制产甲烷菌,从而提高微生物电化学系统合成乙酸产率的可行性,并对比了BES添加前后阴极室微生物菌群结构的变化。结果表明,厌氧混合菌接种物未经BES处理时甲烷是电化学系统CO₂还原的主导产物,最大生成速率达0.95 mmol·L^-1·d^-1,8 d反应时间甲烷中电子回收率达55.0%,16S rRNA测序结果显示固态阴极的主要菌群为Methanobacteriaceae。BES的添加基本抑制了产甲烷菌的活动,使得乙酸成为主导产物,其合成速率最高达2.22 mmol·L^-1·d^-1,系统总电子回收率达67.3%。Rhodocyclaceae (15.1%),Clostridiaceae(11.9%)、Comamonadaceae(11.1%)和Sphingobacteriales(11.0%)为主要菌群。研究结果表明了微生物电化学合成系统中抑制甲烷生成对调控微生态结构,从而调控电化学终产物的重要性。

关键词: 微生物电化学合成系统, 二氧化碳还原, 乙酸合成, 2-溴乙烷磺酸钠(BES), 甲烷化抑制剂, 控制, 选择性, 生物过程

Abstract:

In this study, the specific reduction of CO₂ to acetate in presence of methanogenesis inhibitor 2-bromoethanesulfonate (BES) was studied in a bio-electrochemical system (BES) via a two stage experimental design. During first stage using untreated mixed anaerobic consortia, the methanogenesis was dominated and the CO₂ reduction yielded methane at the maximum rate of 0.95 mmol·L^-1·d^-1at nearly 55.0% coulombic recovery. Sequences belonging to the family Methanobacteriaceae were dominant at the cathodic electrode. During second stage, BES addition selectively suppressed the growth of methanogens, which resulted in a shift of the dominant activity to acetogenesis with the maximum production rate of 2.22 mmol·L^-1·d^-1 with a recovery of 67.3% of electrons in acetate and hydrogen after two duplicates. The main populations were Rhodocyclaceae (15.1%), Clostridiaceae (11.9%), Comamonadaceae (11.1%) and Sphingobacteriales (11.0%). This study highlighted the importance of inhibition of methanogenesis to manoeuvre microbial structures, which decided the final product profiles during a microbial electro synthesis operation.

Key words: microbial electrosynthesis systems (MES), carbon dioxide reduction, acetogenesis, 2-bromoethanesulfonate (BES), methanogenesis inhibition, control, selectivity, boiprocess

中图分类号:

TQ151.5⁺2

戚玉娇, BRIDIER Arnaud, DESMOND LE QUEMENER Elie, 吕凡, 何品晶, BOUCHEZ Théodore. 甲烷化抑制剂在微生物电化学合成乙酸系统中的生物抑制效应[J]. 化工学报, 2016, 67(5): 2033-2040.

QI Yujiao, BRIDIER Arnaud, DESMOND LE QUEMENER Elie, LÜ Fan, HE Pinjing, BOUCHEZ Théodore. Selective inhibition of methanogens using 2-bromoethanesulfonate for improvement of acetate production from CO₂ in bioelectrochemical systems[J]. CIESC Journal, 2016, 67(5): 2033-2040.

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甲烷化抑制剂在微生物电化学合成乙酸系统中的生物抑制效应

Selective inhibition of methanogens using 2-bromoethanesulfonate for improvement of acetate production from CO₂ in bioelectrochemical systems

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甲烷化抑制剂在微生物电化学合成乙酸系统中的生物抑制效应

Selective inhibition of methanogens using 2-bromoethanesulfonate for improvement of acetate production from CO2 in bioelectrochemical systems

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Selective inhibition of methanogens using 2-bromoethanesulfonate for improvement of acetate production from CO₂ in bioelectrochemical systems