化工学报 ›› 2016, Vol. 67 ›› Issue (S2): 245-254.DOI: 10.11949/j.issn.0438-1157.20160576

• 表面与界面工程 • 上一篇    下一篇

电沉积石墨烯的非共价功能化用于SPR核酸传感的协同增敏

袁培新1, 郑晨昱1, 崔宏达1, 万莹2, 姚传广1, 宋宏鑫1, 邓盛元1   

  1. 1. 南京理工大学环境与生物工程学院, 江苏 南京 210094;
    2. 南京理工大学机械工程学院, 江苏 南京 210094
  • 收稿日期:2016-05-03 修回日期:2016-07-14 出版日期:2016-12-30 发布日期:2016-12-30
  • 通讯作者: 邓盛元
  • 基金资助:

    国家自然科学基金项目(21305067);江苏省自然科学基金项目(BK20130754);教育部高等学校博士点基金之新教师类课题(0133219120019)。

Noncovalent functionalization of graphene for sensitizing SPR-based DNA sensing synergistically with biocatalytic polymerization

YUAN Peixin1, ZHENG Chenyu1, CUI Hongda1, WAN Ying2, YAO Chuanguang1, SONG Hongxin1, DENG Shengyuan1   

  1. 1. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu, China;
    2. School of Mechanical Engineering, Nanjing 210094, Jiangsu, China
  • Received:2016-05-03 Revised:2016-07-14 Online:2016-12-30 Published:2016-12-30
  • Supported by:

    supported by the National Natural Science Foundation of China(21305067,61371039),the Natural Science Foundation of Jiangsu Province(BK20130754),Ph.D.Fund of Ministry of Education for Young Teachers(0133219120019).

摘要:

研究了一种高效的表面等离子共振(SPR)基DNA分析方法,利用非共价功能化的石墨烯纳米片作为基底,并以酶催化诱导的聚合作为质量中继。该策略有多方面的目标:首先,通过原位优化的石墨烯薄膜的电生成,该过程由原子力显微拓扑图表征,来敏化总体的SPR输出;其次,用于调制镍离子螯合的胺基三乙酸支架,其吸附在石墨烯支撑的苝基衍生物表面,上端生物素化捕获探针可以自组装,并保有一定的方向;最后,通过辣根过氧化物酶标记的报告单元,实时地将苯胺添加剂转化为聚苯胺沉淀,以协同实现信号的放大。运用上述配置,获得了一个对特异性DNA靶标精确且可重现传感的平台,检测下限达到飞摩尔水平,从而展现了以二维纳米材料为独特SPR基础设施的有益探索和开发。该“自下而上”的建构、与置顶“自上而下”的重量反应器,极有可能拓展并移植用于蛋白质的定量。

关键词: 表面等离子体共振, 脱氧核糖核酸, 电化学还原石墨烯纳米片, 微反应器, 苝接枝胺基三乙酸, 聚合

Abstract:

A highly efficient surface plasmon resonance(SPR)-based DNA assay is developed,by employing noncovalently functionalized graphene nanosheets as the substrate,and enzymatic catalysis-induced polymerization as the mass relay.The objective of this strategy was manifold:first of all,to sensitize the overall SPR output by in situ optimized electrogeneration of graphene thin-film,that was characterized by atomic force micro-topography; secondly,to regulate the self-assembly and orientation of biotinylated capture probes on nickel-chelated nitrilotriacetic acid scaffolds,which were anchored onto graphene-supported pyrenyl derivatives; and lastly,to synergize the signal amplification via real-time conversion of the additive aniline into polyaniline precipitation by horseradish peroxidase-tagged reporters. With this setup,a precise and replicable DNA sensing platform for specific targets was achieved with a detection limit down to femtomolar,demonstrating a beneficial exploration and exploitation of two-dimensional nanomaterials as unique SPR infrastructure.The possibility of such “bottom-up” architecture mounted with “top-down” weight reactor would be most likely extensible and adaptable to protein determinations.

Key words: surface plasmon resonance, DNA, electrochemically reduced graphene nanosheets, microreactor, pyrene-tethered nitrilotriacetic acid, polymerization

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