富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究

doi:10.11949/j.issn.0438-1157.20180219

化工学报 ›› 2018, Vol. 69 ›› Issue (8): 3619-3625.DOI: 10.11949/j.issn.0438-1157.20180219

富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究

李殿鑫^1,2, 胡南^1,2, 黄超^1,2, 丁德馨^1,2, 李广悦^1,2, 王永东^1,2

1 南华大学铀矿冶生物技术国防重点学科实验室, 湖南衡阳 421001;
2 南华大学极贫铀资源绿色开发技术湖南省重点实验室, 湖南衡阳 421001

收稿日期:2018-02-28 修回日期:2018-04-09 出版日期:2018-08-05 发布日期:2018-08-05
通讯作者: 丁德馨
基金资助:
国家自然科学基金项目（11775106，U1401231，51274124）。

Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater

LI Dianxin^1,2, HU Nan^1,2, HUANG Chao^1,2, DING Dexin^1,2, LI Guangyue^1,2, WANG Yongdong^1,2

1 Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, Hunan, China;
2 Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources, University of South China, Hengyang 421001, Hunan, China

Received:2018-02-28 Revised:2018-04-09 Online:2018-08-05 Published:2018-08-05
Supported by:
supported by the National Natural Science Foundation of China (11775106, U1401231, 51274124).

摘要/Abstract

摘要：

通过添加富集得到的含有丰富的硫酸盐还原菌的沉积物到沉积物-地下水微模型中，并添加乙醇作为碳源，分别与接种硫酸盐还原菌并添加碳源组和仅添加碳源组相比较，研究富集的硫酸盐还原菌沉积物对地下水中U（Ⅵ）还原的作用。结果表明，添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组中的铀浓度分别在第19、22和28 d下降至GB 23727-2009规定的0.05 mg·L^-1排放标准以下。各组沉积物中具有还原U（Ⅵ）功能的微生物群落的多样性差异较小。添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组的沉积物中具有还原U（Ⅵ）功能的微生物群落的丰度分别为35.3%、32.5%和13.1%。由此可见，富集的硫酸盐还原菌沉积物增加了微模型中具有还原U（Ⅵ）功能的微生物群落的丰度，从而促进了U（Ⅵ）的还原。

关键词: U(Ⅵ), 废水, 沉积物, 还原, 修复

Abstract:

To study how the incubated sulfate reducing bacteria (SRB) sediment affects U(Ⅵ) bioreduction, the sediment together with ethanol was added into the sediment-groundwater microcosms, which were compared with microcosms amended with both pure SRB and ethanol and with ethanol only. The results indicated that uranium concentrations of the microcosms amended with the incubated SRB sediment together with ethanol, the pure SRB together with ethanol, and ethanol decreased below 0.05 mg·L^-1 (GB 23727-2009) at day 19, 22 and 28, respectively. The diversity of microbial communities capable of reducing U(Ⅵ) in all the microcosms had no significant difference. The abundances of microbial communities capable of reducing U(Ⅵ) in the microcosms amended with the incubated SRB sediment together with ethanol, and the pure SRB together with ethanol, in which ethanol were 35.3%, 32.5% and 13.1%, respectively. The abundance of microbial communities capable of reducing U(Ⅵ) increased when the incubated SRB sediment together with ethanol was added into the sediment-groundwater microcosm. It is indicated that incubated SRB sediment could accelerate the bioreduction of U(Ⅵ) in the microcosm.

Key words: U(Ⅵ), waste water, sediment, reduction, remediation

中图分类号:

李殿鑫, 胡南, 黄超, 丁德馨, 李广悦, 王永东. 富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究[J]. 化工学报, 2018, 69(8): 3619-3625.

LI Dianxin, HU Nan, HUANG Chao, DING Dexin, LI Guangyue, WANG Yongdong. Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater[J]. CIESC Journal, 2018, 69(8): 3619-3625.

参考文献 33

[1]	DING D X, LI S M, HU N, et al. Bioreduction of U(Ⅵ) in groundwater under anoxic conditions from a decommissioned in situ leaching uranium mine[J]. Bioprocess & Biosystems Engineering, 2015, 38(4):661-669.
[2]	ZHANG H, CHENG M, LIU W, et al. Characterization of uranium in the extracellular polymeric substances of anaerobic granular sludge used to treat uranium-contaminated groundwater[J]. RSC Advances, 2017, 7(85):54188-54195.
[3]	CAMACHO L M, DENG S, PARRA R R. Uranium removal from groundwater by natural clinoptilolite zeolite:effects of pH and initial feed concentration[J]. Journal of Hazardous Materials, 2010, 175(1/2/3):393-398.
[4]	DING D, XU F, HU N, et al. Uranium speciation in sediments in microcosms before and after incubation under anoxic conditions[J]. Journal of Radioanalytical and Nuclear Chemistry, 2015, 303(1):925-933.
[5]	TANG G, WATSON D B, WU W, et al. U(Ⅵ) bioreduction with emulsified vegetable oil as the electron donor-model application to a field test[J]. Environmental Science & Technology, 2013, 47(7):3218-3225.
[6]	LUO W, WU W, YAN T, et al. Influence of bicarbonate, sulfate, and electron donors on biological reduction of uranium and microbial community composition[J]. Applied Microbiology and Biotechnology, 2007, 77(3):713-721.
[7]	GIHRING T M, ZHANG G, BRANDT C C, et al. A limited microbial consortium is responsible for extended bioreduction of uranium in a contaminated aquifer[J]. Applied and Environmental Microbiology, 2011, 77(17):5955-5965.
[8]	LUO J, WEBER F, CIRPKA O A, et al. Modeling in-situ uranium(Ⅵ) bioreduction by sulfate-reducing bacteria[J]. Journal of Contaminant Hydrology, 2007, 92(1):129-148.
[9]	SANI R K, PEYTON B M, AMONETTE J E, et al. Reduction of uranium(Ⅵ) under sulfate-reducing conditions in the presence of Fe(Ⅲ)-(hydr)oxides[J]. Geochimica et Cosmochimica Acta, 2004, 68(12):2639-2648.
[10]	LI D, HU N, DING D, et al. An experimental study on the inhibitory effect of high concentration bicarbonate on the reduction of U(Ⅵ) in groundwater by functionalized indigenous microbial communities[J]. Journal of Radioanalytical and Nuclear Chemistry, 2016, 307(2):1011-1019.
[11]	ZHOU C. The versatile roles of sulfate-reducing bacteria for uranium bioremediation[D]. Arizona State University, 2014.
[12]	ZHANG P, HE Z, NOSTRAND J D V, et al. Dynamic succession of groundwater sulfate-reducing communities during prolonged reduction of uranium in a contaminated aquifer[J]. Environmental Science & Technology, 2017, 51(7):3609-3620.
[13]	SHENG L, FEIN J B. Uranium reduction by Shewanella oneidensis MR-1 as a function of NaHCO3 concentration:surface complexation control of reduction kinetics[J]. Environmental Science & Technology, 2014, 48(7):3768-3775.
[14]	GILSON E R, HUANG S, JAFFÉ P R. Biological reduction of uranium coupled with oxidation of ammonium by Acidimicrobiaceae bacterium A6 under iron reducing conditions[J]. Biodegradation, 2015, 26(6):475-482.
[15]	SHELOBOLINA E S, VRIONIS H A, FINDLAY R H, et al. Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium bioremediation[J]. International Journal of Systematic and Evolutionary Microbiology, 2008, 58(Pt 5):1075-1078.
[16]	SHENG L, SZYMANOWSKI J, FEIN J B. The effects of uranium speciation on the rate of U(Ⅵ) reduction by Shewanella oneidensis MR-1[J]. Geochimica et Cosmochimica Acta, 2011, 75(12):3558-3567.
[17]	KAVITHA S, SELVAKUMAR R, SATHISHKUMAR M, et al. Nitrate removal using Brevundimonas diminuta MTCC 8486 from ground water[J]. Water Science & Technology. A Journal of the International Association on Water Pollution Research, 2009, 60(2):517-524.
[18]	SENKO J M, ZHANG G, MCDONOUGH J T, et al. Metal reduction at low pH by a Desulfosporosinus species:implications for the biological treatment of acidic mine drainage[J]. Geomicrobiology Journal, 2009, 26(2):71-82.
[19]	HWANG C, WU W, GENTRY T J, et al. Bacterial community succession during in situ uranium bioremediation:spatial similarities along controlled flow paths[J]. ISME Journal, 2008, 3(1):47-64.
[20]	SANI R K, PEYTON B M, DOHNALKOVA A. Toxic effects of uranium on Desulfovibrio desulfuricans G20[J]. Environmental Toxicology and Chemistry, 2006, 25(5):1231-1238.
[21]	刘晓波, 董少刚, 刘白薇, 等. 内蒙古土默川平原地下水水文地球化学特征及其成因[J]. 地球学报, 2017, 38(6):919-929. LIU X B, DONG S G, LIU B W, et al. Hydrogeoehemical characteristics and genesis of groundwater in the Tumochuan plain of Inner Mongolia[J]. Acta Geoscientica Sinica, 2017, 38(6):919-929.
[22]	LEIGH M B, WU W, CARDENAS E, et al. Microbial communities biostimulated by ethanol during uranium (Ⅵ) bioremediation in contaminated sediment as shown by stable isotope probing[J]. Frontiers of Environmental Science & Engineering, 2015, 9(3):453-464.
[23]	MOON H S, MCGUINNESS L, KUKKADAPU R K, et al. Microbial reduction of uranium under iron-and sulfate-reducing conditions:effect of amended goethite on microbial community composition and dynamics[J]. Water Research, 2010, 44(14):4015-4028.
[24]	SAKAGUCHI S, KANO K, IKEDA T. Effect of pH on the hydrogenase activity of Desulfovibrio vulgaris[J]. Electroanalysis, 2004, 16(13/14):1166-1171.
[25]	KAVITHA S, SELVAKUMAR R, SATHISHKUMAR M, et al. Nitrate removal using Brevundimonas diminuta MTCC 8486 from ground water[J]. Water Science and Technology, 2009, 60(2):517-524.
[26]	SU W, ZHANG L, LI D, et al. Dissimilatory nitrate reduction by Pseudomonas alcaliphila with an electrode as the sole electron donor[J]. Biotechnology & Bioengineering, 2012, 109(11):2904-2910.
[27]	LÜCKER S, WAGNER M, MAIXNER F, et al. A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria[J]. Proceedings of the National Academy of Sciences, 2010, 107(30):13479-13484.
[28]	FLETCHER K E, BOYANOV M I, THOMAS S H, et al. U(Ⅵ) reduction to mononuclear U(Ⅳ) by Desulfitobacterium Species[J]. Environmental Science & Technology, 2010, 44(12):4705-4709.
[29]	O'LOUGHLIN E J, KELLY S D, COOK R E, et al. Reduction of uranium(Ⅵ) by mixed iron(Ⅱ)/iron(Ⅲ) hydroxide (green rust):formation of UO2 nanoparticles[J]. Environmental Science & Technology, 2003, 37(4):721-727.
[30]	JUNIER P, SUVOROVA E I, BERNIERLATMANI R. Effect of competing electron acceptors on the reduction of U(Ⅵ) by Desulfotomaculum reducens[J]. Geomicrobiology Journal, 2010, 27(5):435-443.
[31]	PRAKASH O, GIHRING T M, DALTON D D, et al. Geobacter daltonii sp. nov., an Fe(Ⅲ)-and uranium(Ⅵ)-reducing bacterium isolated from a shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination[J]. International Journal of Systematic and Evolutionary Microbiology, 2010, 60(3):546-553.
[32]	ANDERSON R T, VRIONIS H A, ORTIZBERNAD I, et al. Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer[J]. Appl. Environ. Microbiol., 2003, 69(10):5884-5891.
[33]	BRILEYA K A, CAMILLERI L B, ZANE G M, et al. Biofilm growth mode promotes maximum carrying capacity and community stability during product inhibition syntrophy[J]. Frontiers in Microbiology, 2014, 5(693):1-13.

富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究

Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 33

相关文章 15

编辑推荐

Metrics

本文评价

[1]	程成, 段钟弟, 孙浩然, 胡海涛, 薛鸿祥. 表面微结构对析晶沉积特性影响的格子Boltzmann模拟[J]. 化工学报, 2023, 74(S1): 74-86.
[2]	范孝雄, 郝丽芳, 范垂钢, 李松庚. LaMnO₃/生物炭催化剂低温NH₃-SCR催化脱硝性能研究[J]. 化工学报, 2023, 74(9): 3821-3830.
[3]	杨百玉, 寇悦, 姜峻韬, 詹亚力, 王庆宏, 陈春茂. 炼化碱渣湿式氧化预处理过程DOM的化学转化特征[J]. 化工学报, 2023, 74(9): 3912-3920.
[4]	杨欣, 彭啸, 薛凯茹, 苏梦威, 吴燕. 分子印迹-TiO₂光电催化降解增溶PHE废水性能研究[J]. 化工学报, 2023, 74(8): 3564-3571.
[5]	张琦钰, 高利军, 苏宇航, 马晓博, 王翊丞, 张亚婷, 胡超. 碳基催化材料在电化学还原二氧化碳中的研究进展[J]. 化工学报, 2023, 74(7): 2753-2772.
[6]	李盼, 马俊洋, 陈志豪, 王丽, 郭耘. Ru/α-MnO₂催化剂形貌对NH₃-SCO反应性能的影响[J]. 化工学报, 2023, 74(7): 2908-2918.
[7]	张谭, 刘光, 李晋平, 孙予罕. Ru基氮还原电催化剂性能调控策略[J]. 化工学报, 2023, 74(6): 2264-2280.
[8]	朱理想, 罗默也, 张晓东, 龙涛, 余冉. 醌指纹法指示三氯乙烯污染土功能微生物活性应用研究[J]. 化工学报, 2023, 74(6): 2647-2654.
[9]	张艳梅, 袁涛, 李江, 刘亚洁, 孙占学. 高效SRB混合菌群构建及其在酸胁迫条件下的性能研究[J]. 化工学报, 2023, 74(6): 2599-2610.
[10]	胡南, 陶德敏, 杨照岚, 王学兵, 张向旭, 刘玉龙, 丁德馨. 铁炭微电解与硫酸盐还原菌耦合修复铀尾矿库渗滤水的研究[J]. 化工学报, 2023, 74(6): 2655-2667.
[11]	张兰河, 赖青燚, 王铁铮, 关潇卓, 张明爽, 程欣, 徐小惠, 贾艳萍. H₂O₂对SBR脱氮效率和污泥性能的影响[J]. 化工学报, 2023, 74(5): 2186-2196.
[12]	黄磊, 孔令学, 白进, 李怀柱, 郭振兴, 白宗庆, 李平, 李文. 油页岩添加对准东高钠煤灰熔融行为影响的研究[J]. 化工学报, 2023, 74(5): 2123-2135.
[13]	闫新龙, 黄志刚, 胡清勋, 张新, 胡晓燕. Cu/Co掺杂多孔炭活化过硫酸盐降解水中硝基酚研究[J]. 化工学报, 2023, 74(3): 1102-1112.
[14]	查坦捷, 杨涵, 秦荷杰, 关小红. 仿生材料的构建及其在水环境化学领域中的研究进展[J]. 化工学报, 2023, 74(2): 585-598.
[15]	李鑫, 曾少娟, 彭奎霖, 袁磊, 张香平. CO₂电催化还原制合成气研究进展及趋势[J]. 化工学报, 2023, 74(1): 313-329.