化工学报

• 分离工程 • 上一篇    下一篇

介孔TiO2对不同蛋白的选择性固定化性能

董依慧1, 安蓉2, 庄伟3, 姚忠4, 朱育丹1, 刘畅1, 陆小华1   

  1. 1 南京工业大学材料化学工程国家重点实验室, 江苏 南京 210009;
    2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
    3 南京工业大学生物与制药学院, 江苏 南京 211816;
    4 南京工业大学食品与轻工学院, 江苏 南京 210009
  • 收稿日期:2014-01-02 修回日期:2014-03-10 出版日期:2014-05-05 发布日期:2014-05-05
  • 通讯作者: 刘畅
  • 基金资助:

    国家自然科学基金项目(21136004);国家重点基础研究发展计划项目(2013CB733501);江苏高校优势学科建设工程项目。

Mesoporous TiO2 with different selectivities of protein immobilization performance

DONG Yihui1, AN Rong2, ZHUANG Wei3, YAO Zhong4, ZHU Yudan1, LIU Chang1, LU Xiaohua1   

  1. 1 State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China;
    2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
    3 School of Biological and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
    4 School of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, Jiangsu, China
  • Received:2014-01-02 Revised:2014-03-10 Online:2014-05-05 Published:2014-05-05
  • Supported by:

    supported by the National Natural Science Foundation of China(21136004), the National Basic Research Program of China(2013CB733501)and the Project of Priority Academic Program Development of Jiangsu High Education Institutions(PAPD).

摘要: 通过500℃下焙烧H2Ti2O5制备介孔TiO2-500作为高效液相色谱的固定相填料,测试牛血清蛋白(BSA)、肌红蛋白(myoglobin)、溶菌酶(lysozyme)在不同pH环境下的停留时间分布,结果表明,在pH=4.7,三种蛋白质几乎都固定化,在pH=7.4和11条件下,三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO2-500表面的宏观常压吸附量的结果与色谱结果相一致,介孔TiO2-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此,该介孔TiO2对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。

关键词: 介孔TiO2 , 蛋白质, 色谱, 停留时间, 固定化

Abstract: Mesoporous TiO2 with adjustable pore diameter, high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO2-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H2Ti2O5 at 500℃, and the TiO2 samples were characterized by X-ray diffraction (XRD), N2 adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence time under different pH of bovine serum albumin, myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7, and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO2-500 under atmospheric pressure was consistent with chromatographic results, and the order of the immobilization capacity of mesoporous TiO2-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore, due to the significant difference in protein immobilization performance, the mesoporous TiO2 has a potential application in protein adsorption and separation, immobilizing enzyme and other fields.

Key words: mesoporous TiO2, protein, chromatograph, residence time, immobilization

中图分类号: