介孔TiO2对不同蛋白的选择性固定化性能

doi:10.3969/j.issn.0438-1157.2014.05.027

化工学报

介孔TiO₂对不同蛋白的选择性固定化性能

董依慧¹, 安蓉², 庄伟³, 姚忠⁴, 朱育丹¹, 刘畅¹, 陆小华¹

1 南京工业大学材料化学工程国家重点实验室, 江苏南京 210009;
2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
3 南京工业大学生物与制药学院, 江苏南京 211816;
4 南京工业大学食品与轻工学院, 江苏南京 210009

收稿日期:2014-01-02 修回日期:2014-03-10 出版日期:2014-05-05 发布日期:2014-05-05
通讯作者: 刘畅
基金资助:
国家自然科学基金项目（21136004）；国家重点基础研究发展计划项目（2013CB733501）；江苏高校优势学科建设工程项目。

Mesoporous TiO₂ with different selectivities of protein immobilization performance

DONG Yihui¹, AN Rong², ZHUANG Wei³, YAO Zhong⁴, ZHU Yudan¹, LIU Chang¹, LU Xiaohua¹

1 State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China;
2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
3 School of Biological and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
4 School of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, Jiangsu, China

Received:2014-01-02 Revised:2014-03-10 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Natural Science Foundation of China(21136004), the National Basic Research Program of China(2013CB733501)and the Project of Priority Academic Program Development of Jiangsu High Education Institutions(PAPD).

摘要/Abstract

摘要： 通过500℃下焙烧H₂Ti₂O₅制备介孔TiO₂-500作为高效液相色谱的固定相填料，测试牛血清蛋白（BSA）、肌红蛋白（myoglobin）、溶菌酶（lysozyme）在不同pH环境下的停留时间分布，结果表明，在pH=4.7，三种蛋白质几乎都固定化，在pH=7.4和11条件下，三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO₂-500表面的宏观常压吸附量的结果与色谱结果相一致，介孔TiO₂-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此，该介孔TiO₂对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。

关键词: 介孔TiO₂, 蛋白质, 色谱, 停留时间, 固定化

Abstract: Mesoporous TiO₂ with adjustable pore diameter, high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO₂-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H₂Ti₂O₅ at 500℃, and the TiO₂ samples were characterized by X-ray diffraction (XRD), N₂ adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence time under different pH of bovine serum albumin, myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7, and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO₂-500 under atmospheric pressure was consistent with chromatographic results, and the order of the immobilization capacity of mesoporous TiO₂-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore, due to the significant difference in protein immobilization performance, the mesoporous TiO₂has a potential application in protein adsorption and separation, immobilizing enzyme and other fields.

Key words: mesoporous TiO₂, protein, chromatograph, residence time, immobilization

中图分类号:

董依慧, 安蓉, 庄伟, 姚忠, 朱育丹, 刘畅, 陆小华. 介孔TiO₂对不同蛋白的选择性固定化性能[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.027.

DONG Yihui, AN Rong, ZHUANG Wei, YAO Zhong, ZHU Yudan, LIU Chang, LU Xiaohua. Mesoporous TiO₂ with different selectivities of protein immobilization performance[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.027.

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介孔TiO₂对不同蛋白的选择性固定化性能

Mesoporous TiO₂ with different selectivities of protein immobilization performance

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