粘质沙雷氏菌HB-4吸附重金属镉的机制

doi:10.11949/j.issn.0438-1157.20161427

化工学报 ›› 2017, Vol. 68 ›› Issue (4): 1574-1581.DOI: 10.11949/j.issn.0438-1157.20161427

粘质沙雷氏菌HB-4吸附重金属镉的机制

陈亚奎¹, 徐粲然², 朱启法³, 黄魏魏³, 卢滇楠², 董建江⁴, 刘永民¹

1 辽宁石油化工大学化学化工与环境学院, 辽宁抚顺 113001;
2 清华大学化学工程系, 北京 100084;
3 安徽皖南烟叶责任有限公司, 安徽宣城 242000;
4 中国烟草总公司安徽省公司, 安徽合肥 230022

收稿日期:2016-10-10 修回日期:2016-12-24 出版日期:2017-04-05 发布日期:2017-04-05
通讯作者: 刘永民,卢滇楠
基金资助:
国家自然科学基金项目(21276139)。

Cadmium adsorption mechanism of Serratia marcescens HB-4

CHEN Yakui¹, XU Canran², ZHU Qifa³, HUANG Weiwei³, LU Diannan², DONG Jianjiang⁴, LIU Yongmin¹

1 College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001 Liaoning, China;
2 Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
3 Anhui Wannan Tobacco Liability Company, Xuancheng 242000, Anhui, China;
4 China Tobacco Corporation, Anhui Province, Hefei 230022, Anhui, China

Received:2016-10-10 Revised:2016-12-24 Online:2017-04-05 Published:2017-04-05
Supported by:
supported by the National Natural Science Foundation of China (21276139).

摘要/Abstract

摘要：

从重金属污染土壤中筛选出1株对Cd²⁺具有高耐受能力和高吸附容量的菌株HB-4，经16S rDNA序列分析鉴定为粘质沙雷氏菌（Serratia marcescens）。该菌株能在Cd²⁺浓度为300 mg·L^-¹的条件下正常生长；对Cd²⁺的最大吸附量为（154.7±0.9（ mg·g^-1。考察了Cd²⁺初始浓度、pH、盐浓度以及共存离子对HB-4吸附Cd²⁺的影响，结果表明：pH=3.0~8.0时，对吸附效果无影响；NaCl含量为8.0%时，菌株对Cd²⁺的去除率仍可达到49.9%±0.1%；Pb²⁺、Zn²⁺、Cu²⁺与Cd²⁺共存时，几种重金属离子的去除率分别为98.7%±0.2%（Pb²⁺）、44.6%±0.6%（Zn²⁺）、52.7%±0.1%（Cu²⁺）和64.2%±0.3%（Cd²⁺）。解吸实验证明了HB-4对Cd²⁺极强的吸附能力，洗脱液pH=7.0时，解吸率小于2%。检测了细胞内外镉的分布情况，并利用SEM、XPS和FTIR对吸附机理进行了研究，推断HB-4对Cd²⁺的吸附机理为胞外吸附和胞内摄取。

关键词: 镉污染, 吸附剂, 粘质沙雷氏菌, 吸附作用, 解吸, 胞外吸附, 胞内摄取

Abstract:

The strain HB-4 was screened from heavy metal contaminated soil, identified as Serratia marcescens via 16S rDNA sequence analysis, and had a strong tolerance for Cd²⁺. This strain can grow well when the concentration of Cd²⁺ was up to 300 mg·L^-1 and in that case the maximum adsorption capacity of Cd²⁺ was (154.7±0.9) mg·g^-1. The effect factors of the adsorption of Cd²⁺ by using HB-4 were investigated, including the initial concentration of Cd²⁺, pH, salt concentration and coexisting ions. The results showed that there was no difference in the adsorption capacity when pH was ranged from 3.0 to 8.0. The same results can also be obtained at 8.0% NaCl solution and the removal efficiency were 98.7%±0.2% (Pb²⁺), 44.6%±0.6% (Zn²⁺), 52.7%±0.1% (Cu²⁺) and 64.2%±0.3% (Cd²⁺), respectively, when Pb²⁺, Zn²⁺, Cu²⁺ and Cd²⁺ coexisted in solution. According to desorption experiment, the desorption rate was less than 2% and it demonstrated that HB-4 performed the best on adsorption of Cd²⁺. The distribution of intra-and extracellular cadmium was studied, as well as the results from SEM, XPS and FTIR. It suggested that the mechanism of adsorption of Cd²⁺ by HB-4 contained extracellular adsorption and intracellular uptake.

Key words: cadmium pollution, adsorbent, Serratia marcescens, adsorption, desorption, extracellular adsorption, intracellular uptake

中图分类号:

陈亚奎, 徐粲然, 朱启法, 黄魏魏, 卢滇楠, 董建江, 刘永民. 粘质沙雷氏菌HB-4吸附重金属镉的机制[J]. 化工学报, 2017, 68(4): 1574-1581.

CHEN Yakui, XU Canran, ZHU Qifa, HUANG Weiwei, LU Diannan, DONG Jianjiang, LIU Yongmin. Cadmium adsorption mechanism of Serratia marcescens HB-4[J]. CIESC Journal, 2017, 68(4): 1574-1581.

参考文献 33

[1]	ZHANG S J, YU H Q, FENG H M. PVA-based activated carbon fibers with lotus root-like axially porous structure[J]. Carbon, 2006, 44(10):2059-2068.
[2]	刘丽君. 水环境中镉污染处理的研究进展[J]. 环境科学与管理, 2012, 37(6):124-127. LIU L J. Cadmium pollution treatment in water environment research progress[J]. Environmental Science and Management, 2012, 37(6):124-127.
[3]	韦正峥, 张淑杰, 杨瑞星, 等. 2014年我国环境与健康事件网络舆情分析[J]. 环境与健康杂志, 2015, 32(5):446-447.WEI Z Z, ZHANG S J, YANG R X, et al. Network public opinion analysis about our country environment and the health event in 2014[J].Journal of Environment and Health, 2015, 32(5):446-447.
[4]	环境保护部, 国土资源部. 全国土壤污染状况调查公报[J]. 中国环保产业, 2014, (5):10-11. The Environmental Protection Department, The Ministry of Land and Resources. National soil pollution condition investigation communique[J]. China Environmental Protection Industry, 2014, (5):10-11.
[5]	徐粲然, 卢滇楠, 刘永民. 生物钝化修复镉污染土壤研究进展[J]. 化工进展, 2014, 33(8):2174-2179.XU C R, LU D N, LIU Y M. Research advance in the biostabilization remediation of cadmium contaminated soil[J]. Chemical Industry and Engineering Progress, 2014, 33(8):2174-2179.
[6]	CRISAFI F, GENOVESE M, SMEDILE F, et al. Bioremediation technologies for polluted seawater sampled after an oil-spill in Taranto Gulf (Italy):a comparison of biostimulation, bioaugmentation and use of a washing agent in microcosm studies[J]. Marine Pollution Bulletin, 2016, 106(1/2):119-126.
[7]	GHOSH A. Recent advances in bioremediation of heavy metals and metal complex dyes:review[J]. Journal of Environmental Engineering, 2015. DOI:10.1061/(ASCE)EE. 1943-7870.0000965.
[8]	ESPINOSA-ORTIZ E J, RENE E R, PAKSHIRAJAN K, et al. Fungal pelleted reactors in wastewater treatment:applications and perspectives[J]. Chemical Engineering Journal, 2016, 283:553-571.
[9]	AL-GHEETHI A A S, LALUNG J, NOMAN E A, et al. Removal of heavy metals and antibiotics from treated sewage effluent by bacteria[J]. Clean Technologies & Environmental Policy, 2015, 17(8):1-23.
[10]	ZHENG S, WANG C, SHEN Z, et al. Role of extrinsic arbuscular mycorrhizal fungi in heavy metal-contaminated wetlands with various soil moisture levels[J]. International Journal of Phytoremediation, 2015, 17(1-6):208-214.
[11]	魏运民, 李巧玲, 胡留杰, 等. 墨汁鬼伞对重金属铅离子的耐受与富集作用及其在铅离子胁迫下的差异表达蛋白鉴定[J]. 环境科学学报, 2016, 36(6):1998-2004. WEI Y M, LI Q L, HU L J, et al.Lead tolerance and accumulation in Coprinus atramentarius and identification of differential expression proteins under lead stress[J]. Acta Scientiae Circumstantiae, 2016, 36(6):1998-2004.
[12]	BANERJEE G, PANDEY S, RAY A K, et al. Bioremediation of heavy metals by a novel bacterial strain Enterobacter cloacae, and its antioxidant enzyme activity, flocculant production, and protein expression in presence of lead, cadmium, and nickel[J]. Water Air & Soil Pollution, 2015, 226(4):1-9.
[13]	HASHIM M A, CHU K H. Biosorption of cadmium by brown, green, and red seaweeds[J]. Chemical Engineering Journal, 2004, 97(2):249-255.
[14]	ZIAGOVA M, DIMITRIADIS G, ASLANIDOU D, et al. Comparative study of Cd(Ⅱ) and Cr(Ⅵ) biosorption on Staphylococcus xylosus, and Pseudomonas sp. in single and binary mixtures[J]. Bioresource Technology, 2007, 98(15):2859-2865.
[15]	OZDEMIR G, CEYHAN N, OZTURK T, et al. Biosorption of chromium(Ⅵ), cadmium(Ⅱ) and copper(Ⅱ) by Pantoea sp. TEM18[J]. Chemical Engineering Journal, 2004, 102(3):249-253.
[16]	WU G, SUN M S, LIU P, et al. Enterococcus faecalis, strain LZ-11 isolated from Lanzhou reach of the Yellow River is able to resist and absorb cadmium[J]. Journal of Applied Microbiology, 2014, 116(5):1172-1180.
[17]	LU W B, SHI J J, WANG C H, et al. Biosorption of lead, copper and cadmium by an indigenous isolate Enterobacter sp. J1 possessing high heavy-metal resistance[J]. Journal of Hazardous Materials, 2006, 134(1/2/3):80-86.
[18]	SAY R, YILMAZ N, DENIZLI A. Removal of heavy metal ions using the fungus Penicillium canescens[J]. Adsorptionence & Technology, 2003, 21(7):643-650.
[19]	韩慧龙, 汤晶, 江皓, 等. 真菌-细菌修复石油污染土壤的协同作用机制研究[J]. 环境科学, 2008, 29(1):189-195. HAN H L, TANG J, JIANG H, et al. Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleum-contaminated soil[J]. Environmental Science, 2008, 29(1):189-195.
[20]	KHANNA A, KHANNA M, AGGARWAL A. Serratia marcescens-a rare opportunistic nosocomial pathogen and measures to limit its spread in hospitalized patients[J]. Journal of Clinical & Diagnostic Research, 2013, 7(2):243-246.
[21]	PAN M Y, SHEN Y C, LU C H, et al. Prodigiosin activates endoplasmic reticulum stress cell death pathway in human breast carcinoma cell lines[J]. Toxicology & Applied Pharmacology, 2012, 265(3):325-334.
[22]	肖刚, 洪国强, 陈月燕, 等. 临床标本中粘质沙雷氏菌的鉴定及药敏分析[J]. 实用医技杂志, 2006, 13(22):3974-3975.XIAO G, HONG G Q, CHEN Y Y, et al. In clinical specimens of Serratia marcescens identification and susceptibility analysis[J]. Journal of Practical Medical Techniques, 2006, 13(22):3974-3975.
[23]	AMARO F, TURKEWITZ A P, MARTIN-GONZALEZ A, et al. Whole-cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila[J]. Microbial Biotechnology, 2011, 4(4):513-522.
[24]	TRIPATHI M, MUNOT H P, SHOUCHE Y, et al. Isolation and functional characterization of siderophore-producing lead-and cadmium-resistant Pseudomonas putida KNP9[J]. Current Microbiology, 2005, 50(5):233-237.
[25]	SIVA KIRAN R. Bioaccumulation of cadmium in blue green algae Spirulina (Arthrospira) Indica[J]. Journal of Bioremediation & Biodegradation, 2012, (3):141. doi:10.4172/2155-6199.1000141.
[26]	KIRAN I, AKAR T, OZCAN A S, et al. Biosorption kinetics and isotherm studies of Acid Red 57 by dried Cephalosporium aphidicola, cells from aqueous solutions[J]. Biochemical Engineering Journal, 2006, 31(3):197-203.
[27]	MARTINS R J E, PARDO R, RUI A R B. Cadmium(Ⅱ) and zinc(Ⅱ) adsorption by the aquatic moss Fontinalis antipyretica:effect of temperature, pH and water hardness[J]. Water Research, 2004, 38(3):693-699.
[28]	CRISTAN M, NACCARI C, NOSTRO A, et al. Possible use of Serratia marcescens in toxic metal biosorption (removal)[J]. Environmental Science & Pollution Research International, 2012, 19(1):161-168.
[29]	XUE C, QI P, LI M, et al. Characterization and sorptivity of the Plesiomonas shigelloides strain and its potential use to remove Cd2+ from wastewater[J]. Water, 2016, 8(6):1-12.
[30]	TUZUN I, BAYRAMOGLU G, YALCIN E, et al. Equilibrium and kinetic studies on biosorption of Hg(Ⅱ), Cd(Ⅱ) and Pb(Ⅱ) ions onto microalgae Chlamydomonas reinhardtii[J]. Journal of Environmental Management, 2005, 77(2):85-92.
[31]	MESEGUER V F, ORTUÑO J F, AGUILAR M I, et al. Biosorption of cadmium (Ⅱ) from aqueous solutions by natural and modified non-living leaves of Posidonia oceanica[J]. Environmental Science and Pollution Research, 2016, 23:24032-24046.
[32]	WU H Q, WU Q P, WU G J, et al. Cd-resistant strains of B. cereus S5 with endurance capacity and their capacities for cadmium removal from cadmium-polluted water.[J]. PloS One, 2016, 11(4):1-25.
[33]	黄飞. 蜡状芽孢杆菌对水体中镉的吸附特性与机理研究[D]. 广州:华南理工大学, 2013.HUANG F. Study on the removal of Cd(Ⅱ) from aqueous solution by Bacillus cereus RC-1:biosorption characteristics and mechanism[D]. Guangzhou:South China University of Technology, 2013.

粘质沙雷氏菌HB-4吸附重金属镉的机制

Cadmium adsorption mechanism of Serratia marcescens HB-4

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 33

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨学金, 杨金涛, 宁平, 王访, 宋晓双, 贾丽娟, 冯嘉予. 剧毒气体PH₃的干法净化技术研究进展[J]. 化工学报, 2023, 74(9): 3742-3755.
[2]	盛冰纯, 于建国, 林森. 铝基锂吸附剂分离高钠型地下卤水锂资源过程研究[J]. 化工学报, 2023, 74(8): 3375-3385.
[3]	王皓, 唐思扬, 钟山, 梁斌. MEA吸收CO₂富液解吸过程中固体颗粒表面的强化作用分析[J]. 化工学报, 2023, 74(4): 1539-1548.
[4]	肖川宝, 李林洋, 刘武锋, 钟年丙, 解泉华, 钟登杰, 常海星. 光催化与离子交换吸附耦合有效去除2，4，6-三氯苯酚[J]. 化工学报, 2023, 74(4): 1587-1597.
[5]	潘煜, 王子航, 王佳韵, 王如竹, 张华. 基于可得然-氯化锂复合吸附剂的除湿换热器热湿性能研究[J]. 化工学报, 2023, 74(3): 1352-1359.
[6]	李敏, 阎雪茹, 刘新磊. 苯并咪唑连接聚合物吸附剂和膜研究进展[J]. 化工学报, 2023, 74(2): 599-616.
[7]	王洒, 温怡静, 郭丹煜, 周欣, 李忠. 锆基MOF次级结构单元调控及轻烃吸附分离性能增强[J]. 化工学报, 2022, 73(2): 730-738.
[8]	王玉杰, 李申辉, 赵之平. M-MOF-74吸附分离H₂/He混合物的分子模拟研究[J]. 化工学报, 2022, 73(10): 4507-4517.
[9]	吴俊晔, 葛天舒, 吴宣楠, 代彦军, 王如竹. 基于吸附剂/木浆纤维纸耦合材料的空气净化[J]. 化工学报, 2021, 72(S1): 520-529.
[10]	罗伟莉, 王雯雯, 潘权稳, 葛天舒, 王如竹. 基于活性碳纤维毡复合吸附剂的储热性能[J]. 化工学报, 2021, 72(S1): 554-559.
[11]	安美燕, 王洁冰, 徐震原, 王如竹. 基于LiCl溶液太阳能界面蒸发的连续式空气取水[J]. 化工学报, 2021, 72(S1): 70-76.
[12]	冯宇, 张鑫, 张曼, 王建成, 阎智锋, 李甫, 费鹏飞, 卢建军, 米杰. 静电纺丝纤维对煤基气体污染物脱除研究进展[J]. 化工学报, 2021, 72(8): 3933-3945.
[13]	邓超和, 王佳韵, 李金凤, 刘业凤, 王如竹. 可低温驱动的凝胶复合吸附剂的制备及吸/脱附性能研究[J]. 化工学报, 2021, 72(8): 4401-4409.
[14]	梁苏卓成, 姬国勋, 孙新利, 王波, 张仕通, 代星. 硅杂原子提升冠醚对锂离子络合能力的机理理论研究[J]. 化工学报, 2021, 72(6): 3149-3159.
[15]	王琪, 赵有璟, 刘洋, 王云昊, 王敏, 项顼. 高镁锂比盐湖镁锂分离与锂提取技术研究进展[J]. 化工学报, 2021, 72(6): 2905-2921.