角质酶/表面活性剂体系对混合办公废纸的协同脱墨作用

doi:10.11949/j.issn.0438-1157.20170775

化工学报 ›› 2017, Vol. 68 ›› Issue (12): 4750-4755.DOI: 10.11949/j.issn.0438-1157.20170775

角质酶/表面活性剂体系对混合办公废纸的协同脱墨作用

王凤^1,2, 龙柱^1,2,3, 吴美燕^1,2, 陈杰^1,2, 张辉³

1 江南大学纺织服装学院造纸研究室, 江苏无锡 214122;
2 江南大学生态纺织教育部重点实验室, 江苏无锡 214122;
3 南京林业大学江苏省制浆造纸科学与技术重点实验室, 江苏南京 210037

收稿日期:2017-06-15 修回日期:2017-08-15 出版日期:2017-12-05 发布日期:2017-12-05
通讯作者: 龙柱
基金资助:
国家自然科学基金项目（31270633）；江苏省制浆造纸科学与技术重点实验室开放课题项目（201532）。

Deinking of mixed office waste paper by cutinase/surfactant system

WANG Feng^1,2, LONG Zhu^1,2,3, WU Meiyan^1,2, CHEN Jie^1,2, ZHANG Hui³

1 Laboratory of Papermaking, School of Textiles & Clothing, Jiangnan University, Wuxi 214122, Jiangsu, China;
2 Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China;
3 Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received:2017-06-15 Revised:2017-08-15 Online:2017-12-05 Published:2017-12-05
Supported by:
supported by the National Natural Science Foundation of China(31270633).

摘要/Abstract

摘要：

角质酶能够水解可溶性酯、不溶性甘油三酯和各种聚酯，因此可以水解油墨中的连接料，有代替脂肪酶应用于废纸脱墨领域的潜能。利用角质酶和实验室复配得到的表面活性剂协同应用于混合办公废纸脱墨领域，探讨其脱墨效果和最优工艺，并与常用商业脂肪酶进行脱墨效果比较。结果表明，角质酶在酶用量10 U·g^-1，酶处理时间30 min，酶处理温度50℃，表面活性剂用量0.2%的条件下可以达到最优效果。与脂肪酶/表面活性剂以及单独用表面活性剂脱墨相比，角质酶脱墨后纸页的白度与油墨去除率更高，纸页的机械强度也较好。通过纸页性能的对比和扫描电镜（SEM）观察，角质酶的脱墨效果较脂肪酶更佳，角质酶/表面活性剂体系对混合办公废纸脱墨效果较好。

关键词: 角质酶, 表面活性剂, 混合办公废纸, 浮选脱墨

Abstract:

Cutinase is capable of hydrolyzing soluble esters, insoluble triglycerides and various polyesters, and thus it can hydrolyze the binder in the ink and have the potential to replace lipase in the field of waste paper deinking. Cutinase and self-made surfactant was synergically used in deinking of office waste paper. The deinking effect and optimum process were discussed and compared with the conventional commercial lipase. The results showed that the optimal effect of cutinase could be achieved under the conditions of enzyme dosage of 10 U·g^-1, enzyme treatment time of 30 min, enzyme treatment temperature of 50℃ and surfactant dosage of 0.2%. Compared with lipase/surfactant and surfactant deinking, the brightness and ink removal rate of paper after cutinase deinking were higher and the mechanical strength of paper was also greatly improved. By comparison of the paper performance and scanning electron microscopy (SEM) analysis, the deinking effect of cutinase was better than that of lipase.

Key words: cutinase, surfactant, mixed office waste paper, flotation deinking

中图分类号:

TS724

王凤, 龙柱, 吴美燕, 陈杰, 张辉. 角质酶/表面活性剂体系对混合办公废纸的协同脱墨作用[J]. 化工学报, 2017, 68(12): 4750-4755.

WANG Feng, LONG Zhu, WU Meiyan, CHEN Jie, ZHANG Hui. Deinking of mixed office waste paper by cutinase/surfactant system[J]. CIESC Journal, 2017, 68(12): 4750-4755.

参考文献 31

[1]	LEE C K, IBRAHIM D, OMAR I C. Enzymatic deinking of various types of waste paper:efficiency and characteristics[J]. Process Biochemistry, 2013, 48(2):299-305.
[2]	LASHEVA V, ILIEVA B, KOTLAROVA S, et al. Deinking of recycled paper with offset printing through flotation and use of enzymes[J]. Journal of Chemical Technology and Metallurgy, 2013, 48(5):530-534.
[3]	杨普峰, 胡关键, 曾永坚, 等. 生物酶脱墨技术在文化纸中的应用[J]. 造纸化学品, 2009, 21(2):26-28. YANG P F, HU G J, ZENG Y J, et al. Application of enzymatic deinking technology in cultural paper[J]. Paper Chemicals, 2009, 21(2):26-28.
[4]	李宗全, 秦梦华, 王少霞, 等. 混合办公废纸酶法脱墨的研究[J]. 造纸科学与技术, 2003, (3):7-10. LI Z Q, QIN M H, WANG S X, et al. Enzymatic deinking of mixed office waste[J]. Paper Science and Technology, 2003, (3):7-10.
[5]	胡湛波, 王双飞, 黄显南, 等. 混合办公废纸酶法脱墨过程纤维特性的变化[J]. 中国造纸, 2003, (9):13-16. HU Z B, WANG S F, HUANG X N, et al. Change of fiber morphology during enzymatic deinking of mixed office waste[J]. China Pulp & Paper, 2003, (9):13-16.
[6]	黄六莲. 混合办公废纸的酶法脱墨[J]. 福建林学院学报, 2005, 25(1):38-42. HUANG L L. Enzymatic deinking of mixed office waste[J]. Journal of Fujian College of Forestry, 2005, 25(1):38-42.
[7]	PALA H, MOTA M, GAMA F M. Enzymatic versus chemical deinking of non-impact ink printed paper[J]. Journal of Biotechnology, 2004, 108(1):79-89.
[8]	SPIRIDON I, DE A, AZARIAS M. Enzymatic deinking of old newspaper(ONP)[J]. Progress in Paper Recycling, 2005, 14(3):14.
[9]	LEE C K, IBRAHIM D, OMAR I C. Enzymatic deinking of laser printed office waste papers:some governing parameters on deinking efficiency[J]. Bioresource Technology, 2007, 98(8):1684-1689.
[10]	徐龙权, 张运展. 酶法脱墨的发展及研究动向[J]. 中国造纸, 2010, 29(7):62-68. XU L Q, ZHANG Y Z. Development and research trends of enzymatic deinking[J]. China Pulp & Paper, 2010, 29(7):62-68.
[11]	张素风, 张美云, 赵锦良. 废纸酶法脱墨技术的新进展[J]. 中华纸业, 2006, 27(6):42-45. ZHANG S F, ZHANG M Y, ZHAO J L. New advance in enzymatic deinking technology of waste paper[J]. China Pulp & Paper Industry, 2006, 27(6):42-45.
[12]	郭玉晶, 郑洪辉, 郭玉成. 废新闻纸脂肪酶脱墨方法的研究[J]. 造纸化学品, 2012, (2):15-16. GUO Y J, ZHENG H H, GUO Y C. The application of lipase in old newsprint deinking[J]. Paper Chemicals, 2012, (2):15-16.
[13]	尤纪雪, 杨益琴, 赵艳荣. 办公废纸漆酶脱墨的研究[J]. 中华纸业, 2006, 27(2):34-37. YOU J X, YANG Y Q, ZHAO Y R. A study on enzymatic deinking of office waste paper with laccase[J]. China Pulp & Paper Industry, 2006, 27(2):34-37.
[14]	吴淑芳, 丁少军, 李忠正. 内切纤维素酶I在混合办公废纸酶法脱墨中的作用[J]. 林产化学与工业, 2005, 25(2):87-90. WU S F, DING S J, LI Z Z. Function of endoglucanase I in the deinking process of mixed office waste paper[J]. Chemistry & Industry of Forest Products, 2005, 25(2):87-90.
[15]	尤纪雪, 赵艳荣, 叶汉林. 混合办公废纸漆酶/淀粉酶脱墨的研究[J]. 中国造纸学报, 2008, 28(8):116-124. YOU J X, ZHAO Y R, YE H L. Deinking of mixed office wastepaper with laccase/amylase[J]. Transaction of China Pulp and Paper, 2008, 28(8):116-124.
[16]	徐清华, 秦梦华, 傅英娟. 废新闻纸纤维素酶、半纤维素酶与漆酶/介体协同脱墨作用[J]. 化工学报, 2007, 58(12):3152-3157. XU Q H, QIN M H, FU Y J. Deinking of old newsprint by cellulase or hemicellulase combined with laccase/mediator system[J]. Journal of Chemical Industry and Engineering(China), 2007, 58(12):3152-3157.
[17]	XU Q, FU Y, GAO Y, et al. Performance and efficiency of old newspaper deinking by combining cellulase/hemicellulase with laccase-violuric acid system[J]. Waste Management, 2009, 29(5):1486-1490.
[18]	张效宁, 冉琴琴, 张学俊. 角质酶的研究与应用进展[J]. 中国酿造, 2013, 32(11):11-15. ZHANG X N, RAN Q Q, ZHANG X J. Research and application progress on cutinase[J]. China Brewing, 2013, 32(11):11-15.
[19]	张瑶, 陈晟, 吴丹, 等. 角质酶及其在纺织工业中的应用[J]. 中国生物工程杂志, 2010, 30(9):105-109. ZHANG Y, CHEN S, WU D, et al. Cutinase and its application in textile[J]. China Biotechnology, 2010, 30(9):105-109.
[20]	黄庞慧, 王强, 董爱学. 角质酶/角蛋白酶一浴法处理对羊毛性能的影响[J]. 生物学杂志, 2014, (6):94-98. HUANG P H, WANG Q, DONG A X. Effects of cutinase-keratinase treatment in one bath on wool properties[J]. Journal of Biology, 2014, (6):94-98.
[21]	于竹青, 王树根, 王强. 角质酶在全生态羊毛防缩中的作用[J]. 纺织学报, 2012, 33(2):85-88. YU Z Q, WANG S G, WANG Q. Effects of cutinase on environmentally acceptable shrink-resistant finish of wool[J]. Journal of Textile Research, 2012, 33(2):85-88.
[22]	MARTlNEZ C, NICOLAS A, VAN TILBEURGH H, et al. Cutinase, a lipolytic enzyme with a preformed oxyanion hole[J]. Biochemistry, 1994, 33(1):83-89.
[23]	BAKER P J, POULTNEY C, LIU Z, et al. Identification and comparison of cutinases for synthetic polyester degradation[J]. Appi. Microbiol. Biot., 2012, 93(1):229-240.
[24]	THUMARAT U, NAKAMURA R, KAWABATA T, et al. Biochemical and genetic analysis of cutinase-type polyesterase from a thermophilic Thennobifida alba AHKl19[J]. Appl. Microbiol. Biot., 2012, 95(2):419-430.
[25]	李晓敏, 万金泉, 马邕文. 碱性脂肪酶与表面活性剂协同脱墨[J]. 纸和造纸, 2006, 25(2):49-51. LI X M, WAN J Q, MA Y W. Cooperative deinking of alkali lipase and surfactants[J]. Paper and Paper Making, 2006, 25(2):49-51.
[26]	吴如春, 谢涛, 廖安平. 表面活性剂对酶法脱墨的影响[J]. 纸和造纸, 2007, 26(A1):52-55. WU R C, XIE T, LIAO A P. Effect of surfactant on enzymatic deinking[J]. Paper and Paper Making, 2007, 26(A1):52-55.
[27]	BOBU E, CIOLACU F. Environmental aspects of enzyme deinking[J]. Professional Professional Papermaking, 2007, (1):6-13.
[28]	张玉双, 吴皎萍, 苗庆显, 等. 脂肪酶用于办公废纸脱墨[J]. 福建农林大学学报(自然科学版), 2013, 42(1):106-109. ZHANG Y S, WU J P, MIAO Q X, et al. Lipase-aided deinking of mixed office waste paper[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2013, 42(1):106-109.
[29]	于红, 傅英娟, 汤镇江, 等. 脂肪酶对混合办公废纸脱墨的研究[J]. 纤维素科学与技术, 2002, 10(4):49-52. YU H, FU Y J, TANG Z J, et al. Enzymatic deinking of mixed office waste with lipase[J]. Journal of Cellulose Science and Technology, 2002, 10(4):49-52.
[30]	解金兰, 丛方地. 脂肪酶活性部位上的盖和界面活性[J]. 中国生物化学与分子生物学报, 2011, 27(6):499-504. XIE J L, CONG F D. The lid and interfacial activity of lipases[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(6):499-504.
[31]	SECUNDO F, CARREA G, TARABIONO C, et al. The lid is a structural and functional determinant of lipase activity and selectivity[J]. J. Mol. Catal. B Enzym., 2006, 39(1):166-170.

角质酶/表面活性剂体系对混合办公废纸的协同脱墨作用

Deinking of mixed office waste paper by cutinase/surfactant system

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