聚氨酯泡沫固定化Alcaligenes sp.DN25去除苯酚的研究

doi:10.11949/0438-1157.20201357

摘要/Abstract

摘要：

载体材料是构建固定化体系的基础，其中孔结构直接影响固定化的生物量及降解结果，开展了制备不同孔径聚氨酯泡沫材料并考察其固定化产碱杆菌DN25降解苯酚的研究。结果表明，孔隙结构的聚氨酯泡沫材料在孔径均值为150 μm时所固定的生物量达到最大值（0.0253±0.0010） g，固定化细胞能在48 h内完全降解1160 mg·L^-1苯酚，而游离细胞则完全受到抑制，并且发现载体材料PUF在降解前12 h内吸附苯酚的平衡吸附率为56.1%。进一步研究固定化细胞在初始pH6.0~9.0，NaCl浓度0~4.0%条件下降解900 mg·L^-1苯酚的情况，固定化细胞对苯酚的去除率受pH、NaCl浓度的影响不显著。并且，固定化细胞重复使用11个批次对500 mg·L^-1苯酚的去除率仍能保持100%，反映了PUF-固定化细胞体系对苯酚处理浓度和系统稳定性两方面的强化作用。

关键词: 泡沫, 固定化, 降解, 孔径, 苯酚

Abstract:

Carrier material is the foundation for the construction of immobilized system, in which the pore structure directly affects the immobilized biomass and the degradation results. In this study, different pore sizes of polyurethane foam were synthesized and were used to immobilize Alcaligenes sp. DN25 for phenol degradation. The results showed that the biomass on the polyurethane foam with pore network structure reached the maximum value of (0.0253±0.0010) g when the pore size was 150 μm. Phenol of 1160 mg·L^-1 was completely degraded by the PUF-immobilized cells within 48 h, while the freely suspended cells were completely inhibited. It was found that the equilibrium adsorption rate of 900 mg·L^-1phenol by the PUF carrier was 56.1% within 12 h. Furthermore, phenol degradation by the PUF-immobilized cells under the conditions of initial pH at 6.0—9.0 and NaCl concentration of 0—4.0% were investigated, respectively. There were not significant differences in phenol degradation under the conditions of initial pH and NaCl concentrations. In addition, the removal rate of 500 mg·L^-1 phenol can still be maintained at 100% after the repeated use of 11 batches of immobilized cells, reflecting the enhancement of the PUF-immobilized cell system on both the phenol treatment concentration and system stability.

Key words: foam, immobilized, degradation, pore size, phenol

中图分类号:

X 52

黄文媛, 孙士杰, 唐宏震, 苏智芳, 钟秦迪, 刘幽燕, 李青云. 聚氨酯泡沫固定化Alcaligenes sp.DN25去除苯酚的研究[J]. 化工学报, 2021, 72(5): 2783-2791.

HUANG Wenyuan, SUN Shijie, TANG Hongzhen, SU Zhifang, ZHONG Qindi, LIU Youyan, LI Qingyun. Phenol removal by the Alcaligenes sp. DN25 immobilized on the polyurethane foams[J]. CIESC Journal, 2021, 72(5): 2783-2791.

图/表 8

图1 载体材料固定化前、后的扫描电镜图

Fig.1 Scanning electron microscopy of the carrier material before and after immobilization

图2 游离细胞与PUF-固定化细胞对不同浓度苯酚的降解

Fig.2 Degradation of phenol at different concentrations by the freely suspended cells and PUF-immobilized cells

图3 聚氨酯泡沫材料的孔径分布

Fig.3 Pore size distribution of the polyurethane foam materials

图4 不同孔径泡沫固定化细胞量的结果

Fig.4 The biomass immobilized on the foam at different pore sizes

图5 不同孔径PUF-固定化细胞去除苯酚的时间进程

Fig.5 Time curve of phenol removal by the PUF- immobilized cells at different pore sizes

图6 初始 pH对PUF-固定化细胞去除苯酚的影响

Fig.6 Effect of initial pH on phenol removal by the PUF-immobilized cells

图7 NaCl浓度对PUF-固定化细胞去除苯酚的影响

Fig.7 Effect of salt concentrations on phenol removal by the PUF-immobilized cells

图8 PUF-固定化细胞的重复使用

Fig.8 Reuse of the PUF-immobilized cells for phenol removal

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