醌指纹法指示三氯乙烯污染土功能微生物活性应用研究

doi:10.11949/0438-1157.20230271

摘要/Abstract

摘要：

基于对有机污染场地生物修复潜能评价需求，通过微宇宙试验探讨了醌指纹法解析三氯乙烯（TCE）污染土壤功能微生物活性的潜力。为此，探讨了土壤TCE的降解过程，绘制了土壤醌指纹图谱，通过与现有醌的谱库对比解析了醌指纹图谱并筛选出TCE污染土壤具有指示功能菌群活性潜能特征醌。根据总醌浓度（TQ）和特征醌浓度变化，解析了降解过程中活性微生物量变化和功能菌群活性变化并验证了其可分别作为反映活性微生物量和功能微生物活性潜在指标的可行性。结果表明，主要降解期间在0~50 d， TCE的降解率均在65%以上，三组土壤TCE生物降解途径存在差异；UQ-8、UQ-9、MK-6、MK-8为TCE污染土壤中的特征醌；TQ和微生物量碳（MTOC）、特征醌总浓度与脱氢酶活性均存在显著正相关（P<0.05）。因此，醌指纹法可为有机污染场地监测自然衰减风险管控或生物修复的实施提供重要的监测方法指导。

关键词: 有机化合物, 污染, 修复, 醌指纹法, 微生物活性

Abstract:

This study aimed to evaluate the potential of bioremediation of organic polluted sites using quinone profile method to analyze the activity of functional microbial in trichloroethylene (TCE)-contaminated soil. Microcosm experiments were conducted to investigate the degradation process of TCE in soil and to create a quinone profile. The profile was compared with existing quinone spectral libraries, and a quinone with potential indicator microbial activity for TCE-contaminated soil was selected. By analyzing the changes of the total quinone concentration (TQ) and the concentration of the selected quinone, changes in the active microbial population and functional microbial activity during the degradation process were observed and validated as feasible indicators for reflecting potential microbial activity. The results indicated that primary degradation occurred during 0—50 days, with a degradation rate of TCE over 65% for all three soil groups. Differences in the biodegradation pathways of TCE were observed in the three soil groups. UQ-8, UQ-9, MK-6, and MK-8 were identified as characteristic quinones in TCE-contaminated soil. There were significant positive correlations between TQ, microbial biomass carbon (MTOC), total concentration of characteristic quinones and dehydrogenase activity (P<0.05). Therefore, the quinone profile method can provide an important monitoring method guidance for the monitoring of natural attenuation risk control or the implementation of bioremediation in organically polluted sites.

Key words: organic compounds, contamination, remediation, quinone profile method, microbial activity

中图分类号:

X 53

朱理想, 罗默也, 张晓东, 龙涛, 余冉. 醌指纹法指示三氯乙烯污染土功能微生物活性应用研究[J]. 化工学报, 2023, 74(6): 2647-2654.

Lixiang ZHU, Moye LUO, Xiaodong ZHANG, Tao LONG, Ran YU. Application of quinone profile method to indicate structure and activity of functional microbial community in trichloroethylene-contaminated soil[J]. CIESC Journal, 2023, 74(6): 2647-2654.

图/表 7

表1 三组土壤理化性质背景

Table 1 Background values for the physical and chemical properties of the three groups of soils

组别	含水率/%		氧化还原电位(ORP)/mV		TCE/(μg/kg)
组别	均值	标准差	均值	标准差	均值	标准差
A	22.5	2.1	-25	2	2.2	1.2
B	26.9	1.2	-10	3	3.1	0.8
C	42.9	1.2	-80	9	4.8	1.3

图1 土壤中TCE浓度变化

Fig.1 Variation in TCE concentration in soil

图2 土壤醌指纹图谱随时间的变化

Fig.2 Soil quinone profile vs time

图3 土壤TQ随时间的变化

Fig.3 TQ of soil vs time

图4 三组土壤TQ与MTOC的关系

Fig.4 Relationship between TQ and MTOC for three groups of soils

图5 土壤特征醌总浓度谱随时间的变化

Fig.5 Soil characteristic quinone total concentration profile vs time

图6 三组土壤特征醌总浓度与脱氢酶活性的关系

Fig.6 Relationship between total concentration of characteristic quinone and dehydrogenase activity for three groups of soils

参考文献 32

1	Beamer P I, Luik C E, Abrell L, et al. Concentration of trichloroethylene in breast milk and household water from Nogales, Arizona[J]. Environmental Science & Technology, 2012, 46(16): 9055-9061.
2	生态环境部, 国家市场监督管理总局. 土壤环境质量—建设用地土壤污染风险管控标准: [S]. 北京: 中国标准出版社, 2018.
	State Administration for Market Regulation, Ministry of Ecology and Environment. Soil environmental quality—risk control standard for soil contamination of development land: [S]. Beijing: Standards Press of China, 2018.
3	刘国华, 叶正芳, 吴为中. 土壤微生物群落多样性解析法: 从培养到非培养[J]. 生态学报, 2012, 32(14): 4421-4433.
	Liu G H, Ye Z F, Wu W Z. Culture-dependent and culture-independent approaches to studying soil microbial diversity[J]. Acta Ecologica Sinica, 2012, 32(14): 4421-4433.
4	朱菲莹, 肖姬玲, 张屹, 等. 土壤微生物群落结构研究方法综述[J]. 湖南农业科学, 2017, 385(10): 112-115, 120.
	Zhu F Y, Xiao J L, Zhang Y, et al. Summary of methods on soil microbial community structure[J]. Hunan Agricultural Sciences, 2017, 385(10): 112-115, 120.
5	Hiraishi A. Isoprenoid quinones as biomarkers of microbial populations in the environment[J]. Journal of Bioscience and Bioengineering, 1999, 88(5): 449-460.
6	Hedrick D B, White D C. Microbial respiratory quinones in the environment[J]. Journal of Microbiological Methods, 1986, 5(5/6): 243-254.
7	Abby S S, Kazemzadeh K, Vragniau C, et al. Advances in bacterial pathways for the biosynthesis of ubiquinone[J]. Biochimica et Biophysica Acta—Bioenergetics, 2020, 1861(11): 148259.
8	Becker K W, Elling F J, Schröder J M, et al. Isoprenoid quinones resolve the stratification of redox processes in a biogeochemical continuum from the photic zone to deep anoxic sediments of the black sea[J]. Applied and Environmental Microbiology, 2018, 84(10): e02736-e02717.
9	Collins M D, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication[J]. Microbiological Reviews, 1981, 45(2): 316-354.
32	Teng Y, Luo Y M, Li Z G. Kinetics characters of soil urease, acid phosphotase and dehydrogenase activities in soil contaminated with mixed heavy metals[J]. China Environmental Science, 2008, 28(2): 147-152.
10	Fujie K, Hu H Y, Tanaka H, et al. Analysis of respiratory quinones in soil for characterization of microbiota[J]. Soil Science and Plant Nutrition, 1998, 44(3): 393-404.
11	Hurley S J, Elling F J, Könneke M, et al. Influence of ammonia oxidation rate on thaumarchaeal lipid composition and the TEX₈₆ temperature proxy[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(28): 7762-7767.
12	李荣, 宋维峰. 哈尼梯田生态系统土壤微生物量碳的影响因素[J]. 生态学报, 2020, 40(17): 6223-6232.
	Li R, Song W F. Factors affecting soil microbial biomass carbon in Hani terraced ecosystem[J]. Acta Ecologica Sinica, 2020, 40(17): 6223-6232.
13	陈果. 淹水土壤中微生物生物量碳测定方法的研究[D]. 杭州: 浙江大学, 2007.
	Chen G. Studies on methods for measuring microbial biomass C in waterlogged soils[D]. Hangzhou: Zhejiang University, 2007.
14	戴濡伊, 吴季荣, 徐剑宏, 等. 小麦根际土壤脱氢酶活性测定方法的改进[J]. 江苏农业学报, 2013, 29(4): 772-776.
	Dai R Y, Wu J R, Xu J H, et al. Improvement of determination dehydrogenase activity in wheat rhizospheric soil[J]. Jiangsu Journal of Agricultural Sciences, 2013, 29(4): 772-776.
15	刘倩, 刘永杰, 余辉, 等. 太湖流域土地利用与河流水质污染状况的相关性研究[J]. 环境工程, 2016, 34(8): 11-17.
	Liu Q, Liu Y J, Yu H, et al. The correlation study of land use and water quality pollution condition in Taihu Lake watershed[J]. Environmental Engineering, 2016, 34(8): 11-17.
16	向交, 徐丽萍, 李和平, 等. 氧化还原电位的研究及应用[J]. 地球与环境, 2014, 42(3): 430-436.
	Xiang J, Xu L P, Li H P, et al. Research on and application of oxidation-reduction potential[J]. Earth and Environment, 2014, 42(3): 430-436.
17	Liang Y, Liu X K, Singletary M A, et al. Relationships between the abundance and expression of functional genes from vinyl chloride (VC)-degrading bacteria and geochemical parameters at VC-contaminated sites[J]. Environmental Science & Technology, 2017, 51(21): 12164-12174.
18	胡海珠, 毛晓敏. 地下水高浓度三氯乙烯厌氧生物降解的进展[J]. 科技导报, 2010, 28(21): 112-117.
	Hu H Z, Mao X M. Review of anaerobic dechlorination of high concentrated trichloroethylene in groundwater[J]. Science & Technology Review, 2010, 28(21): 112-117.
19	Fuller M E, Mu D Y, Scow K M. Biodegradation of trichloroethylene and toluene by indigenous microbial populations in vadose sediments[J]. Microbial Ecology, 1995, 29(3): 311-325.
20	陈翠柏, 杨琦, 沈照理. 地下水三氯乙烯(TCE)生物修复的研究进展[J]. 华东地质学院学报, 2003, 26(1): 10-14, 37.
	Chen C B, Yang Q, Shen Z L. Foreign progression on trichloroethylene (TCE) bioremediation in groundwater[J]. Journal of East China Geological Institute, 2003, 26(1): 10-14, 37.
21	祝冲之, 余冉, 龙涛, 等. 醌指纹法及其在环境微生物领域的应用[J]. 环境污染与防治, 2022, 44(1): 85-91.
	Zhu C Z, Yu R, Long T, et al. Quinone profile method and its application in the environmental microorganisms field[J]. Environmental Pollution & Control, 2022, 44(1): 85-91.
22	Villanueva L, del Campo J, Guerrero R, et al. Intact phospholipid and quinone biomarkers to assess microbial diversity and redox state in microbial mats[J]. Microbial Ecology, 2010, 60(1): 226-238.
23	Hooper A B, Erickson R H, Terry K R. Electron transport systems of Nitrosomonas: isolation of a membrane-envelope fraction[J]. Journal of Bacteriology, 1972, 110(1): 430-438.
24	Holliger C, Wohlfarth G, Diekert G. Reductive dechlorination in the energy metabolism of anaerobic bacteria[J]. FEMS Microbiology Reviews, 1998, 22(5): 383-398.
25	Yassin A F, Galinski E A, Wohlfarth A, et al. A new actinomycete species, Nocardiopsis lucentensis sp. nov[J]. International Journal of Systematic Bacteriology, 1993, 43(2): 266-271.
26	Mino S, Kudo H, Arai T, et al. Sulfurovum aggregans sp. nov., a hydrogen-oxidizing, thiosulfate-reducing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent chimney, and an emended description of the genus Sulfurovum[J]. International Journal of Systematic and Evolutionary Microbiology, 2014, 64(9): 3195-3201.
27	Katayama A, Funasaka K, Fujie K. Changes in the respiratory quinone profile of a soil treated with pesticides[J]. Biology and Fertility of Soils, 2001, 33(6): 454-459.
28	Olaniran A O, Pillay D, Pillay B. Aerobic biodegradation of dichloroethenes by indigenous bacteria isolated from contaminated sites in Africa[J]. Chemosphere, 2008, 73(1): 24-29.
29	Xiao Z X, Jiang W, Chen D, et al. Bioremediation of typical chlorinated hydrocarbons by microbial reductive dechlorination and its key players: a review[J]. Ecotoxicology and Environmental Safety, 2020, 202: 110925.
30	黎荣彬. 土壤微生物生物量碳研究进展[J]. 广东林业科技, 2008, 24(6): 65-69.
	Li R B. Review of research advances of soil microbial biomass carbon[J]. Guangdong Forestry Science and Technology, 2008, 24(6): 65-69.
31	Saitou K, Nagasaki K I, Yamakawa H, et al. Linear relation between the amount of respiratory quinones and the microbial biomass in soil[J]. Soil Science and Plant Nutrition, 1999, 45(3): 775-778.
32	滕应, 骆永明, 李振高. 土壤重金属复合污染对脲酶、磷酸酶及脱氢酶的影响[J]. 中国环境科学, 2008, 28(2): 147-152.

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