Molecular identification and conditions for preliminary application of a Bacillus sp.

doi:10.3969/j.issn.0438-1157.2013.04.046

CIESC Journal ›› 2013, Vol. 64 ›› Issue (4): 1459-1465.DOI: 10.3969/j.issn.0438-1157.2013.04.046

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Molecular identification and conditions for preliminary application of a Bacillus sp.

WU Xiang¹, QU Yi¹, CHENG Guojun², NIE Wenchao², DU Dongyun¹

1. Institute of Environment Engineering and Science, School of Chemistry and Material Science, Key Laboratory of Catalysis and Materials Science of State Ethnic Affairs Commission & Ministry of Education, South Central University for Nationalities, Wuhan 430074, Hubei, China;
2. School of Life Science, South Central University for Nationalities, Wuhan 430074, Hubei, China

Received:2012-09-18 Revised:2012-12-17 Online:2013-04-05 Published:2013-04-05
Supported by:
supported by Wuhan City Key Project(201160638173).

一株芽孢杆菌的分子鉴定及初步应用条件探究

吴翔¹, 曲毅¹, 程国军², 聂文超², 杜冬云¹

1. 中南民族大学环境科学与工程研究所,中南民族大学化学与材料科学学院/催化材料科学国家民委-教育部共建重点实验室,湖北武汉 430074;
2. 中南民族大学生命科学学院,湖北武汉430074

通讯作者: 杜冬云
作者简介:吴翔(1989—),男,硕士研究生。
基金资助:
武汉市重点项目(201160638173)。

Abstract

Abstract: Ethyl chloride is an important intermediate of pesticide.It is widely used to produce organic phosphorus pesticide with high effect and low toxicity.Toxic wastewater produced in the manufacture process contains high values of chemical oxygen demand, total phosphorus, and sulfur ion.A bacterial strain SCUN which could aerobically degrade ethyl chloride wastewater was isolated from the natural water body near the wastewater discharge facility.This strain was identified as Bacillus sp.SCUN.based on its morphological observation and 16S rDNA sequence analysis.Both indole and hydrogen sulfide were not detected in the experiments of IMViC.Salt tolerance of the microorganism could reach 7%.The condition for its industrial application was discussed.It was found that at pH value of 7.0, immobilized microorganism ratio of 5%, temperature of 30℃, the microorganism showed the best growth for biodegradation.With a newly developed ternary cycle treatment, the COD removal rate approached 58.3%.

Key words: ethyl chloride, wastewater treatment, Bacillus sp., biological characteristics

关键词: 乙基氯化物, 废水处理, 芽孢杆菌, 生理特性

CLC Number:

WU Xiang, QU Yi, CHENG Guojun, NIE Wenchao, DU Dongyun. Molecular identification and conditions for preliminary application of a Bacillus sp.[J]. CIESC Journal, 2013, 64(4): 1459-1465.

吴翔, 曲毅, 程国军, 聂文超, 杜冬云. 一株芽孢杆菌的分子鉴定及初步应用条件探究[J]. 化工学报, 2013, 64(4): 1459-1465.

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Molecular identification and conditions for preliminary application of a Bacillus sp.

一株芽孢杆菌的分子鉴定及初步应用条件探究

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