Abstract: The gel filtration was carried out for purification of cellulase. The influences of
chromatographic parameters on the resolution were studied to determine the optimal
conditions for purification. The purified endoglucanase was obtained by gel filtration by
Superdex 75 prep grade with an activity recovery of 92.8% and the purification factor 4.2.
The sample volume should be below 6 % of the column bed volume and the column bed height L
≥ 12.0 cm. The optimum catalysis temperature and pH for the enzyme were 55 ℃ and 4.5-5.0,
respectively. The cellulase was stable at pH ranging from 4.0 to 6.0 and temperature below
60 ℃.

Key words: cellulase, endoglucanase, purification, characterization, gel chromatography

摘要： The gel filtration was carried out for purification of cellulase. The influences of
chromatographic parameters on the resolution were studied to determine the optimal
conditions for purification. The purified endoglucanase was obtained by gel filtration by
Superdex 75 prep grade with an activity recovery of 92.8% and the purification factor 4.2.
The sample volume should be below 6 % of the column bed volume and the column bed height L
≥ 12.0 cm. The optimum catalysis temperature and pH for the enzyme were 55 ℃ and 4.5-5.0,
respectively. The cellulase was stable at pH ranging from 4.0 to 6.0 and temperature below
60 ℃.

关键词: cellulase;endoglucanase;purification;characterization;gel chromatography