CIESC Journal ›› 2015, Vol. 66 ›› Issue (9): 3640-3648.DOI: 10.11949/j.issn.0438-1157.20151022

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Separation, purification and composition analysis of bio-oil from hydrothermal liquefaction of microalgae

FANG Lina1,2, CHEN Yu2, LIU Ya1, WU Kejing2, WU Yulong2, YANG Mingde2   

  1. 1 College of Food, Shihezi University, Shihezi 832003, Xinjiang, China;
    2 Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China
  • Received:2015-06-30 Revised:2015-07-07 Online:2015-09-05 Published:2015-09-05
  • Supported by:

    supported by the National Natural Science Foundation of China (21376140, 21176142), Program for New Century Excellent Talents in University (NCET-12-0308), and Program for Changjiang Scholars and Innovative Research Team in University (IRT13026).

藻类水热液化产物生物油分离纯化及组分分析

方丽娜1,2, 陈宇2, 刘娅1, 吴可荆2, 吴玉龙2, 杨明德2   

  1. 1 石河子大学食品学院, 新疆 石河子 832003;
    2 清华大学核能与新能源技术研究院, 北京 100084
  • 通讯作者: 吴玉龙
  • 基金资助:

    国家自然科学基金项目(21376140,21176142);教育部新世纪优秀人才支持计划项目(NCET-12-0308);教育部长江学者与创新团队计划(IRT13026)。

Abstract:

In the present paper, the bio-oil produced from hydrothermal liquefaction of D. tertiolecta was divided into n-hexane, chloroform and diethyl ether fractions via solvent division at first, and then chloroform fraction was purified by column chromatography. The n-hexane and diethyl ether fractions were directly analyzed by GC-MS and FT-IR, while the separation products obtained from column chromatography of chloroform fraction were combined with secondary mass spectrometry and FT-IR to confirm the product information. The results showed that chloroform fraction can be divided into sixteen components including the petroleum ether fraction (A1) mainly in alkenes, petroleum ether:ethyl acetate fraction (A2) mainly in carboxylic acid, petroleum ether:acetone fraction (A3) mainly in amides, petroleum ether:methanol fraction (A4) mainly in higher alkanes, and methanol fraction (A5) mainly in amides. In addition, the product recovery was reached up to 91.38% after the purification via column chromatography. Meanwhile, some components, which cannot be directly detected by GC-MS, were identified after column chromatography. The results gave a comprehensive insight in the compositions of product obtained from microalgal liquefaction, which provided the basis on further understanding of HTL mechanism and bio-oil upgrading.

Key words: bio-oil, purification, separation, column chromatography, liquefaction, biomass

摘要:

杜氏盐藻通过水热液化制备得到的生物油,先采用溶剂分割分别得到正己烷相、二氯甲烷相和乙醚相,再对二氯甲烷相进行柱层析分离纯化。正己烷相和乙醚相直接通过GC-MS和FT-IR进行分析,二氯甲烷相先经柱层析分离后结合GC-MS、二次质谱和FT-IR等确认不同馏分的产物组成。研究结果表明:二氯甲烷相经柱层析分离可得到16个馏分,分别是石油醚馏分(A1),主要是烯烃类;石油醚:乙酸乙酯馏分(A2),主要是酸类化合物;石油醚:丙酮馏分(A3),主要是酰胺类;石油醚:甲醇馏分(A4),主要是烷烃类;甲醇馏分(A5),主要是十八碳烯酰胺。经柱层析分离纯化后,生物油的回收率高达91.38%;获取较全的生物油组分信息,为藻类液化机理的分析和生物油的改质提供了依据。

关键词: 生物油, 分离, 纯化, 柱层析, 液化, 生物质

CLC Number: