化工学报 ›› 2015, Vol. 66 ›› Issue (9): 3661-3668.DOI: 10.11949/j.issn.0438-1157.20150925

• 生物化学工程与技术 • 上一篇    下一篇

毕赤酵母-表面活性剂协同作用制备Au纳米线

景孝廉1,2, 黄登坡1, 黄加乐1, 孙道华1, 李清彪1,2,3   

  1. 1 厦门大学化学化工学院化学工程与生物工程系, 醇醚酯化工清洁生产国家工程实验室, 福建 厦门 361005;
    2 厦门大学环境与生态学院, 福建 厦门 361005;
    3 泉州师范学院化学与生命科学学院, 福建 泉州 362000
  • 收稿日期:2015-06-15 修回日期:2015-06-25 出版日期:2015-09-05 发布日期:2015-09-05
  • 通讯作者: 黄加乐, 李清彪
  • 基金资助:

    国家自然科学基金项目(21406186)。

Preparation of Au nanowires based on synergistic action of Pichia pastoris cells and surfactant

JING Xiaolian1,2, HUANG Dengpo1, HUANG Jiale1, SUN Daohua1, LI Qingbiao1,2,3   

  1. 1 Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, National Laboratory for Green Chemical Productions of Alcohols, Ethers and Esters, Xiamen University, Xiamen 361005, Fujian, China;
    2 Environmental Science Research Center, College of Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China;
    3 College of Chemistry & Life Science, Quanzhou Normal University, Quanzhou 362000, Fujian, China
  • Received:2015-06-15 Revised:2015-06-25 Online:2015-09-05 Published:2015-09-05
  • Supported by:

    supported by the National Natural Science Foundation of China (21406186).

摘要:

在毕赤酵母-十六烷基三甲基溴化铵(CTAB)的协同作用下利用抗坏血酸(AA)还原氯金酸(HAuCl4)制备Au纳米线, 采用SEM、TEM、HRTEM及SAED等手段对Au产物进行表征,结果表明产物为带有分支结构的Au纳米线,纳米线在分叉处的晶型是连续的,具有多晶结构的特征。研究发现,菌体-HAuCl4的相互作用对于后续纳米线的生成过程至关重要,部分Au(Ⅲ)可吸附在菌体表面并被还原为很小的Au(0),在AA加入后纳米线的生成过程中起到了晶种的作用。菌体-HAuCl4作用时间过长会导致菌体表面生成的Au(0)趋于稳定,不利于进一步生长为Au纳米线。菌体-表面活性剂的协同作用导致分支结构的多晶Au纳米线的生成。

关键词: Au纳米线, 毕赤酵母, 表面活性剂

Abstract:

Au nanowires (AuNWs) were obtained by the reduction of HAuCl4 with ascorbic acid (AA) under the synergistic action between microorganism (Pichia pastoris cells, PPCs) and hexadecyltrimethylammonium bromides (CTAB). SEM, TEM, HRTEM and SEAD were used to characterize the shape and structure feature of the Au nanoproducts. The results showed the AuNWs were hierarchically branched polycrystal and the interfaces of the branched positions were continuous in lattice. The interaction process between the microorganism and CTAB was researched and it was found that Au(0) could be formed on the PPCs surface, which acted as the seeds for the formation of AuNWs after AA addtion. Without the interaction process between the microorganism and CTAB, the Au nanoparticles instead of nanowires were acquired. Too long time adsorption between PPCs and HAuCl4 led to stable Au(0) on the microorganism surface, which was also unfavorable for the subsequent growth of AuNWs. The synergistic action between microorganism and CTAB resulted in the formation of AuNWs with hierarchically branched polycrystalline structure.

Key words: Au nanowires, Pichia pastoris cells, surfactant

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