TiO2纳米管阵列孔径调控葡萄糖氧化酶生物传感器性能

doi:10.11949/j.issn.0438-1157.20160181

化工学报 ›› 2016, Vol. 67 ›› Issue (10): 4324-4333.DOI: 10.11949/j.issn.0438-1157.20160181

TiO₂纳米管阵列孔径调控葡萄糖氧化酶生物传感器性能

饶超¹, 董依慧¹, 庄伟², 邬新兵¹, 洪启亮¹, 刘畅¹, 陆小华¹

1 南京工业大学材料化学工程国家重点实验室, 江苏南京 210009;
2 南京工业大学国家生化工程技术研究中心, 江苏南京 211816

收稿日期:2016-02-19 修回日期:2016-05-06 出版日期:2016-10-05 发布日期:2016-10-05
通讯作者: 刘畅
基金资助:
国家重点基础研究发展计划项目（2013CB733501）；国家自然科学基金项目（21136004，21476106，21506090）；江苏省自然科学基金项目（BK20130929）；江苏高校优势学科建设工程项目。

Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays

RAO Chao¹, DONG Yihui¹, ZHUANG Wei², WU Xinbing¹, HONG Qiliang¹, LIU Chang¹, LU Xiaohua¹

1 State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China;
2 National Engineering Technique Research Center for Biotechnology, Nanjing Tech University, Nanjing 211816, Jiangsu, China

Received:2016-02-19 Revised:2016-05-06 Online:2016-10-05 Published:2016-10-05
Supported by:
supported by the National Basic Research Program of China (2013CB733501), the National Natural Science Foundation of China (21136004, 21476106, 21506090), the Natural Science Foundation of Jiangsu Province (BK20130929) and the Project of Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要/Abstract

摘要：

采用电化学阳极氧化法制备出不同孔径（21、62、83、102 nm）的TiO₂纳米管阵列（TNA），研究了孔径对固定化葡萄糖氧化酶（GOx）的传感器性能的影响。循环伏安测试结果表明固定在不同孔径大小的TNA上的GOx在葡萄糖溶液中均具有良好的酶活性。计时电流法和交流阻抗法测试发现，当孔径是83 nm时，灵敏度达到最大值27.2 μA·（mmol·L^-1）^-1·cm^-2。调控TNA的孔径可改变固定化GOx的活性及溶液扩散阻抗，从而显著提高生物传感器性能。

关键词: 葡萄糖氧化酶, TiO₂纳米管阵列, 孔径, 扩散阻抗, 电化学, 生物传感器, 制备, 生物过程

Abstract:

Highly ordered TiO₂ nanotube arrays (TNA) with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L^-1)^-1·cm^-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

Key words: glucose oxidase, TiO₂ nanotube arrays, pore size, diffusion resistance, electrochemistry, biosensor, preparation, bioprocess

中图分类号:

饶超, 董依慧, 庄伟, 邬新兵, 洪启亮, 刘畅, 陆小华. TiO₂纳米管阵列孔径调控葡萄糖氧化酶生物传感器性能[J]. 化工学报, 2016, 67(10): 4324-4333.

RAO Chao, DONG Yihui, ZHUANG Wei, WU Xinbing, HONG Qiliang, LIU Chang, LU Xiaohua. Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays[J]. CIESC Journal, 2016, 67(10): 4324-4333.

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TiO₂纳米管阵列孔径调控葡萄糖氧化酶生物传感器性能

Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays

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