关键词:

扩张床, 吸附, 牛血清白蛋白

Abstract: In the first part of this paper, the GET composite copolymer with pendant epoxy groups were prepared. It was reacted with diethylamine, forming an anion-exchange chromatographic medium DEAE-GET. The effects of liquid viscosity, flow velocity on expansion characteristics and liquid mixing performance of DEAE-GET in expanded bed were studied. Its expansion characteristics in STREAMLINE 25 column were found to obey the Richardson-Zaki equation. The increase of liquid viscosity, flow velocity resulted in the increase of axial mixing coefficient. In the range of flow velocity 100—500 cm•h^-1, the low value of axial mixing coefficient in expanded bed (10^-6—10^-5m²•s^-1) indicated that the liquid flow through the expanded bed was close to a plug flow profile. The maximum static adsorption capacity for BSA was found to be 43.0 mg•ml^-1.The breakthrough curves for BSA adsorption on DEAE-GET and STREAMLINE DEAE in packed bed and expanded bed were compared,showing for each adsorbent they were close to each other.The maximum adsorption capacities on DEAE-GET in packed bed and expanded bed were 28.0 mg•ml^-1 and 26.2 mg•ml^-1 respectively.

Key words:

扩张床, 吸附, 牛血清白蛋白