关键词:

DNA, 蛋白质, 迁移, 纳米微孔, 分子模拟

Abstract:

Translocation of charged biological macromolecules such as DNA across biological membranes through a nanopore is one of the most crucial elementary processes of life.Study on the translocation of DNA is helpful to understand the molecular mechanism of life and explore its potential application in rapid DNA sequencing, gene therapy, drug delivery and so on.In this paper, the progresses in the investigation of translocation process of DNA through nanopore by using single-molecule electrophysiology experiments, theory of polymer transport and molecular simulations are reviewed.The translocation dynamics of DNA and its mechanism are paid more attention to, especially, the scaling relationship of translocation time with molecular weight.

Key words:

DNA, 蛋白质, 迁移, 纳米微孔, 分子模拟