介孔TiO2固定化葡萄糖氧化酶的直接电化学性能

doi:10.3969/j.issn.0438-1157.2014.05.031

化工学报

介孔TiO₂固定化葡萄糖氧化酶的直接电化学性能

邬新兵, 蒙萌, 庄伟, 吕玲红, 陆小华

南京工业大学材料化学工程国家重点实验室, 江苏南京 210009

收稿日期:2014-01-02 修回日期:2014-02-24 出版日期:2014-05-05 发布日期:2014-05-05
通讯作者: 吕玲红
基金资助:
国家重点基础研究发展计划项目（2013CB733500）；国家自然科学基金项目（21176113，21136004）。

Direct electrochemistry of glucose oxidase immobilized on mesoporous TiO₂

WU Xinbing, MENG Meng, ZHUANG Wei, LÜ Linghong, LU Xiaohua

State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China

Received:2014-01-02 Revised:2014-02-24 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Basic Research Program of China (2013CB733500) and the National Natural Science Foundation of China (21176113, 21136004).

摘要/Abstract

摘要： 采用非模板软化学法制备了一种孔径均一的介孔TiO₂材料（m-TiO₂）。XRD测试结果表明其晶型为锐钛矿，且结晶度高，通过N₂吸-脱附曲线可看出存在规整的介孔结构且具有高比表面，从FESEM和TEM可以看出制备的m-TiO₂由许多纳米粒子构成微米级大颗粒，且存在均匀分布的介孔。将此种TiO₂材料固定化葡萄糖氧化酶（GOx）后，通过红外光谱（FT-IR）分析可知m-TiO₂材料可以有效固定化GOx。将m-TiO₂作为固定化材料固定化GOx制备成Nafion/GOx/m-TiO₂/GC电极并进行相关电化学测试，测试结果表明m-TiO₂作为固定化材料固定化GOx后具有良好的催化活性，在无电子媒介体存在下能够实现反应电子与电极表面的直接电子传递，检测线性范围为0.1～1.2 mmol·L^-1葡萄糖，灵敏度为3.44μA·mmol^-1·L·cm^-2，在葡萄糖传感领域具有良好的应用前景。

关键词: 介孔TiO₂, 纳米材料, 多孔介质, 葡萄糖氧化酶, 电化学, 生物传感

Abstract: A novel mesoporous TiO₂ material (m-TiO₂) was obtained by a soft chemistry method in the absence of any surfactants or templates. It was shown that the m-TiO₂ had an anatase crystalline structure with well-distributed mesopores from XRD, N₂ adsorption-desorption isotherms, FESEM and TEM. The FT-IR results indicated that glucose oxidase (GOx) could be well immobilized on m-TiO₂. The electrochemical tests of the fabricated Nafion/GOx/m-TiO₂ modified glass carbon electrode (GCE) showed fast direct electron transfer between GOx molecules and electrode surface, exhibiting a linear response to glucose concentration ranging from 0.1 to 1.2 mmol·L^-1, and good sensitivity of 3.44 μA·mmol^-1·L·cm^-2, which proved that the novel m-TiO₂ was a promising material for immobilization of GOx and fabrication of glucose biosensors.

Key words: mesoporous TiO₂, nanomaterials, porous media, glucose oxidase, electrochemistry, biosensor

中图分类号:

邬新兵, 蒙萌, 庄伟, 吕玲红, 陆小华. 介孔TiO₂固定化葡萄糖氧化酶的直接电化学性能[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.031.

WU Xinbing, MENG Meng, ZHUANG Wei, LÜ Linghong, LU Xiaohua. Direct electrochemistry of glucose oxidase immobilized on mesoporous TiO₂[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.031.

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介孔TiO₂固定化葡萄糖氧化酶的直接电化学性能

Direct electrochemistry of glucose oxidase immobilized on mesoporous TiO₂

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