葡萄糖氧化酶在修饰碳纳米管上的电化学

doi:10.3969/j.issn.0438-1157.2014.05.030

化工学报

葡萄糖氧化酶在修饰碳纳米管上的电化学

王佳^1,2, 李俊华², 周健¹

1 华南理工大学化学与化工学院, 绿色化学产品技术广东省重点实验室, 广东广州 510640;
2 陕西省石油化工研究设计院, 陕西西安 710054

收稿日期:2013-12-31 修回日期:2014-02-13 出版日期:2014-05-05 发布日期:2014-05-05
通讯作者: 周健
基金资助:
国家重点基础研究发展计划项目（2013CB733500）；国家自然科学基金项目（21376089，91334202）；中央高校基本科研业务费项目（SCUT-2013ZM0073）。

Electrochemistry of glucose oxidase on modified carbon nanotubes

WANG Jia^1,2, LI Junhua², ZHOU Jian¹

1 Guangdong Provincial Key Laboratory for Green Chemical Product Technology, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China;
2 Shaanxi Research Design Institute of Petroleum and Chemical Industry, Xi’an 710054, Shaanxi, China

Received:2013-12-31 Revised:2014-02-13 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Basic Research Program of China (2013CB733500), the National Natural Science Foundation of China (21376089, 91334202) and the Fundamental Research Funds for the Central Universities (SCUT-2013ZM0073).

摘要/Abstract

摘要： 将葡萄糖氧化酶（GOx）分别固定在多壁碳纳米管（MWNT）、氨基化碳纳米管（AMWNTs）和羧基化碳纳米管（MWNTs-COOH）修饰的电极表面，电化学测量表明固定在羧基和氨基碳纳米管上的GOx式量电位基本没变，而峰电流得到了很大提高。尤其是氨基化碳纳米管上的GOx的峰电流是未功能化碳管上GOx的4倍多。进一步研究Nafion/GOx-AMWNTs/GC电极的电化学行为，发现固定在AMWNTs上的GOx可进行直接准可逆的氧化还原反应，而且固定在AMWNTs上的GOx有良好的稳定性。氨基改性碳纳米管电极载体材料有望显著提高GOx生物燃料电池性能。

关键词: 酶, 固定化, 电化学, 蛋白质, 吸附, 生物燃料电池

Abstract: Glucose oxidase (GOx) was immobilized on the electrode surface of multi-walled carbon nanotubes, amino functionalized carbon nanotubes (AMWNTs) and carboxyl functionalized carbon nanotubes (MWNTs-COOH). Electrochemical measurements indicated that the formal potentials of GOx immobilized on AMWNTs and MWNTs-COOH did not change, but their peak currents were improved. The peak current of GOx immobilized on AMWNTs was four times larger than that immobilized on MWNTs. The electrochemistry behavior of Nafion/GOx-AMWNTs/GC electrode were further characterized. The results indicated that GOx immobilized on AMWNTs could undergo a direct quasi-reversible electrochemical reaction and show good stability. Amino-functionalized electrodes could significantly improve the performance of GOx-based biofuel cells.

Key words: enzyme, immobilization, electrochemistry, protein, adsorption, biofuel cell

中图分类号:

O646

王佳, 李俊华, 周健. 葡萄糖氧化酶在修饰碳纳米管上的电化学[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.030.

WANG Jia, LI Junhua, ZHOU Jian. Electrochemistry of glucose oxidase on modified carbon nanotubes[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.030.

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葡萄糖氧化酶在修饰碳纳米管上的电化学

Electrochemistry of glucose oxidase on modified carbon nanotubes

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