藻类水热液化产物生物油分离纯化及组分分析

doi:10.11949/j.issn.0438-1157.20151022

化工学报 ›› 2015, Vol. 66 ›› Issue (9): 3640-3648.DOI: 10.11949/j.issn.0438-1157.20151022

藻类水热液化产物生物油分离纯化及组分分析

方丽娜^1,2, 陈宇², 刘娅¹, 吴可荆², 吴玉龙², 杨明德²

1 石河子大学食品学院, 新疆石河子 832003;
2 清华大学核能与新能源技术研究院, 北京 100084

收稿日期:2015-06-30 修回日期:2015-07-07 出版日期:2015-09-05 发布日期:2015-09-05
通讯作者: 吴玉龙
基金资助:
国家自然科学基金项目(21376140,21176142);教育部新世纪优秀人才支持计划项目(NCET-12-0308);教育部长江学者与创新团队计划(IRT13026)。

Separation, purification and composition analysis of bio-oil from hydrothermal liquefaction of microalgae

FANG Lina^1,2, CHEN Yu², LIU Ya¹, WU Kejing², WU Yulong², YANG Mingde²

1 College of Food, Shihezi University, Shihezi 832003, Xinjiang, China;
2 Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China

Received:2015-06-30 Revised:2015-07-07 Online:2015-09-05 Published:2015-09-05
Supported by:
supported by the National Natural Science Foundation of China (21376140, 21176142), Program for New Century Excellent Talents in University (NCET-12-0308), and Program for Changjiang Scholars and Innovative Research Team in University (IRT13026).

摘要/Abstract

摘要：

杜氏盐藻通过水热液化制备得到的生物油,先采用溶剂分割分别得到正己烷相、二氯甲烷相和乙醚相,再对二氯甲烷相进行柱层析分离纯化。正己烷相和乙醚相直接通过GC-MS和FT-IR进行分析,二氯甲烷相先经柱层析分离后结合GC-MS、二次质谱和FT-IR等确认不同馏分的产物组成。研究结果表明:二氯甲烷相经柱层析分离可得到16个馏分,分别是石油醚馏分(A₁),主要是烯烃类;石油醚:乙酸乙酯馏分(A₂),主要是酸类化合物;石油醚:丙酮馏分(A₃),主要是酰胺类;石油醚:甲醇馏分(A₄),主要是烷烃类;甲醇馏分(A₅),主要是十八碳烯酰胺。经柱层析分离纯化后,生物油的回收率高达91.38%;获取较全的生物油组分信息,为藻类液化机理的分析和生物油的改质提供了依据。

关键词: 生物油, 分离, 纯化, 柱层析, 液化, 生物质

Abstract:

In the present paper, the bio-oil produced from hydrothermal liquefaction of D. tertiolecta was divided into n-hexane, chloroform and diethyl ether fractions via solvent division at first, and then chloroform fraction was purified by column chromatography. The n-hexane and diethyl ether fractions were directly analyzed by GC-MS and FT-IR, while the separation products obtained from column chromatography of chloroform fraction were combined with secondary mass spectrometry and FT-IR to confirm the product information. The results showed that chloroform fraction can be divided into sixteen components including the petroleum ether fraction (A₁) mainly in alkenes, petroleum ether:ethyl acetate fraction (A₂) mainly in carboxylic acid, petroleum ether:acetone fraction (A₃) mainly in amides, petroleum ether:methanol fraction (A₄) mainly in higher alkanes, and methanol fraction (A₅) mainly in amides. In addition, the product recovery was reached up to 91.38% after the purification via column chromatography. Meanwhile, some components, which cannot be directly detected by GC-MS, were identified after column chromatography. The results gave a comprehensive insight in the compositions of product obtained from microalgal liquefaction, which provided the basis on further understanding of HTL mechanism and bio-oil upgrading.

Key words: bio-oil, purification, separation, column chromatography, liquefaction, biomass

中图分类号:

TQ519

方丽娜, 陈宇, 刘娅, 吴可荆, 吴玉龙, 杨明德. 藻类水热液化产物生物油分离纯化及组分分析[J]. 化工学报, 2015, 66(9): 3640-3648.

FANG Lina, CHEN Yu, LIU Ya, WU Kejing, WU Yulong, YANG Mingde. Separation, purification and composition analysis of bio-oil from hydrothermal liquefaction of microalgae[J]. CIESC Journal, 2015, 66(9): 3640-3648.

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藻类水热液化产物生物油分离纯化及组分分析

Separation, purification and composition analysis of bio-oil from hydrothermal liquefaction of microalgae

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