无定形介孔磷酸锆固定葡萄糖氧化酶的直接电化学

doi:10.11949/j.issn.0438-1157.20151475

化工学报 ›› 2016, Vol. 67 ›› Issue (5): 2161-2168.DOI: 10.11949/j.issn.0438-1157.20151475

• 材料化学工程与纳米技术 • 上一篇下一篇

无定形介孔磷酸锆固定葡萄糖氧化酶的直接电化学

于志辉, 黄鹏飞, 汪夏燕

北京工业大学环境与能源工程学院, 北京 100124

收稿日期:2015-09-21 修回日期:2015-12-03 出版日期:2016-05-05 发布日期:2016-05-05
通讯作者: 于志辉
基金资助:
国家高技术研究发展计划项目(2012AA052201);北京市教育委员会科技发展计划重点项目(KZ201310005001);北京市教委科研计划面上项目(KM201210005008);新世纪优秀人才支持计划项目(NCET-12-0603)。

Direct electrochemistry of immobilized glucose oxidase on amorphous mesoporous zirconium phosphate

YU Zhihui, HUANG Pengfei, WANG Xiayan

College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Received:2015-09-21 Revised:2015-12-03 Online:2016-05-05 Published:2016-05-05
Supported by:
supported by the National High Technology Research and Development Program of China (2012AA052201).

摘要/Abstract

摘要：

利用无定形介孔磷酸锆(ZrP)为载体,通过吸附法固定葡萄糖氧化酶(GOD),修饰玻碳(GC)电极得到GOD/ZrP/GC电极。在0.1 mol·L^-1磷酸盐缓冲溶液中,利用循环伏安法研究了GOD/ZrP/GC电极的直接电化学行为和对葡萄糖的催化性能。结果表明,无定形磷酸锆ZrP为载体修饰的电极GOD/ZrP/GC其电化学反应电子转移速率快、表观覆盖量大;对葡萄糖的检测表现出较快的电流响应和较高的灵敏度,说明无定形磷酸锆更有利于GOD的固定和酶电极的直接电化学。

关键词: 葡萄糖氧化酶, 电催化性能, 催化剂载体, 电化学, 稳定性

Abstract:

Glucose oxidase (GOD)/zirconium phosphate(ZrP)/glassy carbon(GC) electrode was prepared by adsorbing GOD on the amorphous mesoporous zirconium phosphate. The electrocatalytic properties of GOD/ZrP/GC electrode were characterized by cyclic voltammetric method at the medium of phosphate buffer (0.1 mol·L^-1). The results showed that there were quicker electron transfer rate and larger surface coverage when GOD was supported on zirconium phosphate. At the same time, the electrochemical device with this electrode showed faster current response and higher sensitivity in detection of glucose, indicating that zirconium phosphate could be more suitable support for immobilization of GOD and achieve better performance of direct electrochemistry.

Key words: glucose oxidase, electro-catalytic performance, catalyst support, electrochemistry, stability

中图分类号:

O646

于志辉, 黄鹏飞, 汪夏燕. 无定形介孔磷酸锆固定葡萄糖氧化酶的直接电化学[J]. 化工学报, 2016, 67(5): 2161-2168.

YU Zhihui, HUANG Pengfei, WANG Xiayan. Direct electrochemistry of immobilized glucose oxidase on amorphous mesoporous zirconium phosphate[J]. CIESC Journal, 2016, 67(5): 2161-2168.

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无定形介孔磷酸锆固定葡萄糖氧化酶的直接电化学

Direct electrochemistry of immobilized glucose oxidase on amorphous mesoporous zirconium phosphate

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