酵母菌的致孔作用对PVA/CMC水凝胶性能的影响

doi:10.11949/0438-1157.20190895

摘要/Abstract

摘要：

利用酵母菌发泡制备了聚乙烯醇/羧甲基纤维素多孔水凝胶(D-PC)，采用FTIR对其化学结构进行了表征和Zeta电位分析共同证明了酵母菌和材料之间的相互作用；利用SEM和BET分别对其三维网络结构进行了观察以及比表面积、孔径进行了测定；研究了D-PC在不同条件下对亚甲基蓝(MB)的去除，通过纤维素降解酶实验测试了D-PC的降解性。研究结果表明：酵母菌通过静电作用包覆在D-PC内；随着酵母菌量的增加，D-PC的比表面积增大、平均孔径减小；D-PC对MB的去除机制以化学吸附为主，且吸附动力学符合伪二级动力学方程；去除效率随着酵母菌的增加而增大，随着pH、温度的增加先增大后减小；一定条件下D-PC能降解水凝胶中的聚乙烯醇。

关键词: 凝胶, 酵母菌致孔, 吸附作用, 降解, 多功能性

Abstract:

A polyvinyl alcohol/carboxymethyl cellulose porous hydrogel (D-PC) was prepared by yeast foaming. The chemical structure was characterized by FTIR and combined with Zeta potential analysis to prove the interaction between yeast and materials. The three-dimensional network structure was observed by SEM and BET, and the specific surface area and pore size were measured. The effects of D-PC on the removal of the basic dye methylene blue (MB) at different conditions were studied, and the removal mechanism was explored. Degradability of D-PC was tested by cellulose degrading enzyme experiment. The results showed that yeast was encapsulated in D-PC by electrostatic action. As the amount of yeast increases, the specific surface area of D-PC increased and the average pore size decreased. The removal efficiency of MB by D-PC increased with the increase of yeast, and increased first and then decreased with the increase of pH and temperature. The removal mechanism was dominated by chemical adsorption. The kinetic data were fitted to the pseudo-second-order kinetic model reasonably well. Under certain conditions, the yeast could degrade the polyvinyl alcohol in the hydrogel, so the hydrogel had double degradation versatility.

Key words: gel, yeast foaming, absorption, degradation, multifunction

中图分类号:

TQ 325.9

万豫, 张敏, 翁云宣, 李成涛. 酵母菌的致孔作用对PVA/CMC水凝胶性能的影响[J]. 化工学报, 2020, 71(4): 1828-1835.

Yu WAN, Min ZHANG, Yunxuan WENG, Chengtao LI. Effect of porogenic of yeast on adsorption and degradation of PVA/CMC hydrogel[J]. CIESC Journal, 2020, 71(4): 1828-1835.

图/表 8

图1 D-PC1.4及其原料的FTIR光谱图

Fig.1 FTIR spectrum of D-PC1.4 and its raw materials

图2 D-PC1.4、PC和酵母菌在不同pH下的Zeta电位

Fig.2 Zeta potential of D-PC1.4, PC and yeast at different pH

图3 D-PC和PC的SEM图片

Fig.3 SEM images of D-PC and PC

表1 D-PC和PC的BET参数

Table 1 BET parameters of D-PC and PC

样品	比表面积/(m²/g)	平均孔径 / nm
PC	1.56±0.23	12.29±1.21
D-PC_1.0	2.13±0.45	20.23±0.77
D-PC_1.2	2.88±0.31	15.65±1.03
D-PC_1.4	3.08±0.27	14.28±2.00

图4 酵母菌对D-PC水凝胶吸附MB的影响

Fig.4 Effect of yeast on adsorption of MB by D-PC hydrogel

图5 D-PC水凝胶去除MB的机制

Fig.5 D-PC hydrogel mechanism for removing MB

表2 伪一级和伪二级吸附动力学模型参数

Table 2 Pseudo-primary and pseudo-secondary adsorption kinetic model parameters

T/K	q_e,exp/(mg/g)	伪一级动力学			伪二级动力学
T/K	q_e,exp/(mg/g)	k₁/min^-1	q_e,cal/(mg/g)	R₁²	k₂/[g/(mg·min)]	q_e,cal/(mg/g)	R₂²
303	5.12	0.0013	0.18	0.9148	0.013	4.68	0.99

图6 酵母菌对D-PC水凝胶生物降解性的影响以及降解不同时间后D-PC1.4的SEM照片

Fig.6 Effect of yeast on biodegradability of D-PC hydrogel and SEM photograph of D-PC1.4 after degradation at different time

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