内嵌纳凝胶阴离子交换聚甲基丙烯酸羟乙酯复合晶胶分离苯乳酸研究

doi:10.11949/0438-1157.20200623

摘要/Abstract

摘要：

采用超声乳化聚合法制备了聚甲基丙烯酸丁酯纳凝胶，然后经结晶致孔法制备了内嵌纳凝胶的甲基丙烯酸羟乙酯纳晶胶，对其接枝功能单体N，N，N-三甲基乙烯基苯甲氯化铵，得到内嵌纳凝胶阴离子交换纳晶胶，用于从发酵液中分离苯乳酸。结果表明：所得纳晶胶具有良好的渗透性能和轴向分散性能，在流速为1 cm·min^-1时，不同配比纳晶胶对牛血清白蛋白的吸附容量为3.9~5.4 mg·ml^-1；将质量比为 8∶2（HEMA∶pBMA）的纳晶胶用于从转化液中分离苯乳酸，所得苯乳酸的纯度达89.24%，回收率93.74%，分离效果好，表明其在生物分离方面有良好的应用前景。

关键词: 纳凝胶, 纳晶胶, 吸附, 苯乳酸

Abstract:

Hydrophobic nanogels were synthesized by emulsion polymerization method under ultrasound using butyl methacrylate as the monomer. Then, anion exchange nano-cryogels embedded with the obtained nanogels were prepared by cryo-polymerization using poly(2-hydroxyethyl methacrylate) as the monomer, followed by a graft polymerization with the monomer (vinylbenzyl) trimethyl ammonium chloride. The anion exchange nano-cryogels were used to isolate phenyllactic acid from biotransformation broth. The results show that the obtained nanocrystalline gel has good permeability and axial dispersion performance. When the flow rate is 1 cm·min^-1, the adsorption capacity of different ratios of nanocrystalline gel for bovine serum protein is 3.9—5.4 mg·ml^-1. When the nano-cryogel with mass ratio of 8∶2(HEMA∶pBMA) was used to isolate phenyllactic acid from transformation broth, phenyllactic acid with high purity of 89.24% and recovery of 93.74% was obtained, indicating that the nano-cryogels could have potential applications in bioseparation.

Key words: nanogels, nano-cryogels, adsorption, phenyllactic acid

中图分类号:

TQ 028

贺亚维,张颂红,黄杰,刘流,李国华,贠军贤. 内嵌纳凝胶阴离子交换聚甲基丙烯酸羟乙酯复合晶胶分离苯乳酸研究[J]. 化工学报, 2020, 71(12): 5636-5643.

HE Yawei,ZHANG Songhong,HUANG Jie,LIU Liu,LI Guohua,YUN Junxian. Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels[J]. CIESC Journal, 2020, 71(12): 5636-5643.

图/表 11

图1 BMA、EDMA、pBMA的红外谱图

Fig.1 FT-IR spectra of BMA, EDMA, pBMA

图2 pBMA纳凝胶粒径分布

Fig.2 Particle size distribution of pBMA nanogels

图3 阴离子交换型纳晶胶介质扫描电镜图

Fig.3 Scanning electron microscope photographs of anion-exchange nano-cryogels

表1 纳晶胶介质孔隙率

Table 1 The porosities of nano-cryogels

Mass ratios of HEMA to pBMA nanogels	The effective porosity/%	The absolute porosity /%
9:1	82.5	88.0
8:2	81.2	88.8
7:3	79.5	84.9

表2 不同配比的纳晶胶长度和渗透率

Table 2 Permeability and length of the composite cryogel matrix with different mass ratios of HEMA to pBMA nanogels (column inner diameter: 10 mm)

Mass ratios of HEMA to pBMA nanogels	L/cm	k_w×10¹²/m²
9:1	5.0	1.77
8:2	4.7	1.26
7:3	5.2	0.35

图4 纳晶胶介质停留时间分布HEMA与pBMA纳凝胶质量比：(a) 9∶1; (b) 8∶2; (c) 7∶3

Fig.4 Residence time distribution curves in nano-cryogels

图5 纳晶胶介质在不同流速下的HETP值

Fig.5 HETP values of nano-cryogels at various liquid velocities

图6 纳晶胶层析BSA曲线

Fig.6 Chromatographic curves of BSA in the nano-cryogels with different mass ratios of HEMA to pBMA nanogels

图7 纳晶胶介质对PLA的吸附层析过程

Fig.7 Chromatographic adsorption process of PLA in the nano-cryogel

图8 上样液层析过程中PLA的浓度和纯度变化

Fig.8 Concentrations and purities of PLA with the loading liquid volume during the chromatography process of sample solution

图9 层析转化过程中不同阶段流出液的HPLC图

Fig.9 HPLC results of PLA and other contamination contents in the effluent sample for the chromatography process of sample solution

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