化工学报 ›› 2024, Vol. 75 ›› Issue (6): 2274-2282.DOI: 10.11949/0438-1157.20240049
张祎琪1,2(), 谭雪松2, 李吾环1,2, 张权2,3, 苗长林2, 庄新姝1,2()
收稿日期:
2024-01-11
修回日期:
2024-03-07
出版日期:
2024-06-25
发布日期:
2024-07-03
通讯作者:
庄新姝
作者简介:
张祎琪(2000—),女,硕士研究生,2869039897@qq.com
基金资助:
Yiqi ZHANG1,2(), Xuesong TAN2, Wuhuan LI1,2, Quan ZHANG2,3, Changlin MIAO2, Xinshu ZHUANG1,2()
Received:
2024-01-11
Revised:
2024-03-07
Online:
2024-06-25
Published:
2024-07-03
Contact:
Xinshu ZHUANG
摘要:
使用酸性乙二醇苯醚(EPH)体系对甘蔗渣(SCB)进行预处理,在最优条件下(110℃,0.05 mol/L H2SO4,60 min)实现了90.47%的纤维素保留率,去除了90.33%的木质素和76.70%的半纤维素。回收得到的富含纤维素的残渣最高酶解率为89.71%,是未经处理的甘蔗渣的酶解率(15.93%)的近6倍。用异丙醚从预处理液中回收了74.29%的木质素(纯度为93.53%)。使用SEM、XRD、FTIR对预处理后残渣及原料的组成及结构进行表征,发现甘蔗渣的形貌结构被破坏,结晶度提高,因而酶解率大幅度提高。通过2D HSQC表征发现在预处理过程中乙二醇苯醚和木质素的醚化反应抑制了木质素的缩合。本研究建立了酸性乙二醇苯醚预处理体系,实现了甘蔗渣组分的高效分离,并高效回收高纯木质素与可发酵糖,可为生物质高值化利用提供参考。
中图分类号:
张祎琪, 谭雪松, 李吾环, 张权, 苗长林, 庄新姝. 温和条件下乙二醇苯醚高效分离回收甘蔗渣组分[J]. 化工学报, 2024, 75(6): 2274-2282.
Yiqi ZHANG, Xuesong TAN, Wuhuan LI, Quan ZHANG, Changlin MIAO, Xinshu ZHUANG. Efficient fractionation of sugarcane bagasse with phenoxyethanol under mild condition[J]. CIESC Journal, 2024, 75(6): 2274-2282.
温度/℃ | 酸度/(mol/L) | 时间/min | 纤维素保留率/% | 半纤维素去除率/% | 木质素去除率/% | 残渣保留率/% | 酶解率/% |
---|---|---|---|---|---|---|---|
70 | 0.05 | 60 | 97.89 | 1.06 | 3.41 | 95.23 | 18.89 |
90 | 0.05 | 60 | 92.72 | 28.16 | 22.77 | 78.09 | 29.73 |
110 | 0.05 | 60 | 90.47 | 76.70 | 90.33 | 48.54 | 72.79 |
130 | 0.05 | 60 | 87.04 | 100.00 | 92.68 | 40.17 | 76.15 |
表1 不同温度下乙二醇苯醚预处理甘蔗渣的效果和酶解率
Table 1 Effect and cellulose enzymatic digestibility of sugarcane bagasse after EPH pretreatment under different temperature
温度/℃ | 酸度/(mol/L) | 时间/min | 纤维素保留率/% | 半纤维素去除率/% | 木质素去除率/% | 残渣保留率/% | 酶解率/% |
---|---|---|---|---|---|---|---|
70 | 0.05 | 60 | 97.89 | 1.06 | 3.41 | 95.23 | 18.89 |
90 | 0.05 | 60 | 92.72 | 28.16 | 22.77 | 78.09 | 29.73 |
110 | 0.05 | 60 | 90.47 | 76.70 | 90.33 | 48.54 | 72.79 |
130 | 0.05 | 60 | 87.04 | 100.00 | 92.68 | 40.17 | 76.15 |
有机溶剂 | 催化剂 | 温度/℃ | 时间/min | 酶解率/% | 文献 |
---|---|---|---|---|---|
乙二醇苯醚 | 硫酸 | 110 | 60 | 89.71 | 本文 |
乙二醇苯醚/丙酮/水 | 硫酸 | 125 | 120 | 74.52 | [ |
60%乙醇 | FeCl3 | 160 | 60 | 93.80 | [ |
水/乙醇/乙酸乙酯/甲酸 | 甲酸 | 159 | 40 | 84.50 | [ |
甘油 | 硫酸 | 200 | 15 | 70.00 | [ |
表2 不同有机溶剂预处理甘蔗渣的酶解率比较
Table 2 Comparison of enzymatic hydrolysis of sugarcane bagasse with different organic solvent pretreatment
有机溶剂 | 催化剂 | 温度/℃ | 时间/min | 酶解率/% | 文献 |
---|---|---|---|---|---|
乙二醇苯醚 | 硫酸 | 110 | 60 | 89.71 | 本文 |
乙二醇苯醚/丙酮/水 | 硫酸 | 125 | 120 | 74.52 | [ |
60%乙醇 | FeCl3 | 160 | 60 | 93.80 | [ |
水/乙醇/乙酸乙酯/甲酸 | 甲酸 | 159 | 40 | 84.50 | [ |
甘油 | 硫酸 | 200 | 15 | 70.00 | [ |
图4 CEL、EPHL的二维HSQC NMR谱中的侧链区域(δC/δH 45~95/2.5~6.0)和芳香族区域(δC/δH 95~140/6.0~8.5)
Fig.4 Side chain region (δC/δH 45—95/2.5—6.0) and aromatic region (δC/δH 95—140/6.0—8.5) in 2D HSQC NMR spectra of CEL and EPHL
木质素结构单元和基本连接键 | CEL | EPHL |
---|---|---|
S/% | 30.78 | 20.96 |
G/% | 69.22 | 79.04 |
S/G | 0.445 | 0.265 |
β-O-4/% | 53.82 | 19.71 |
β-β/% | 7.12 | 4.41 |
表3 CEL和EPHL二维HSQC核磁共振光谱中芳香族和脂肪族区域的半定量分析
Table 3 Semi-quantitative analysis of aromatic and aliphatic regions in the 2D HSQC NMR spectra of CEL and EPHL
木质素结构单元和基本连接键 | CEL | EPHL |
---|---|---|
S/% | 30.78 | 20.96 |
G/% | 69.22 | 79.04 |
S/G | 0.445 | 0.265 |
β-O-4/% | 53.82 | 19.71 |
β-β/% | 7.12 | 4.41 |
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