Efficient fractionation of sugarcane bagasse with phenoxyethanol under mild condition

doi:10.11949/0438-1157.20240049

Abstract

Abstract:

In this study, efficient fractionation of sugarcane bagasse (SCB) with acidified phenoxyethanol (EPH) was investigated. High lignin (90.33%) and hemicellulose (76.70%) removal efficiency with 90.47% cellulose retention was achieved by using EPH at 110℃, 60 min, with 0.05 mol/L acid loading for SCB. The enzymatic digestibility of residue reached 89.71%, which was nearly 6 times that of the untreated sugarcane bagasse (15.93%). 74.29% lignin of 93.53% purification was recovered from the pretreatment solution using isopropyl ether. SEM, XRD, and FTIR were used to characterize the composition and structure of the pretreated residue and raw materials. It was found that the morphological structure of bagasse was destroyed, the crystallinity was improved, and the enzymatic digestibility was greatly improved. Additionally, the etherification of phenoxyethanol and lignin to suppress the condensation of lignin was disclosed by 2D HSQC characterization. Overall, EPH pretreatment achieved efficient fractionation and recovery of high purity lignin and fermentable sugars under mild condition from sugarcane bagasse. This study can provide reference for the high-value utilization of lignocellulosic biomass.

Key words: biomass, bioseparation, pretreatment, enzymatic hydrolysis, lignin, bioenergy

摘要：

使用酸性乙二醇苯醚（EPH）体系对甘蔗渣（SCB）进行预处理，在最优条件下（110℃，0.05 mol/L H₂SO₄，60 min）实现了90.47%的纤维素保留率，去除了90.33%的木质素和76.70%的半纤维素。回收得到的富含纤维素的残渣最高酶解率为89.71%，是未经处理的甘蔗渣的酶解率（15.93%）的近6倍。用异丙醚从预处理液中回收了74.29%的木质素（纯度为93.53%）。使用SEM、XRD、FTIR对预处理后残渣及原料的组成及结构进行表征，发现甘蔗渣的形貌结构被破坏，结晶度提高，因而酶解率大幅度提高。通过2D HSQC表征发现在预处理过程中乙二醇苯醚和木质素的醚化反应抑制了木质素的缩合。本研究建立了酸性乙二醇苯醚预处理体系，实现了甘蔗渣组分的高效分离，并高效回收高纯木质素与可发酵糖，可为生物质高值化利用提供参考。

关键词: 生物质, 生物分离, 预处理, 酶解, 木质素, 生物能源

CLC Number:

TK 6

Yiqi ZHANG, Xuesong TAN, Wuhuan LI, Quan ZHANG, Changlin MIAO, Xinshu ZHUANG. Efficient fractionation of sugarcane bagasse with phenoxyethanol under mild condition[J]. CIESC Journal, 2024, 75(6): 2274-2282.

张祎琪, 谭雪松, 李吾环, 张权, 苗长林, 庄新姝. 温和条件下乙二醇苯醚高效分离回收甘蔗渣组分[J]. 化工学报, 2024, 75(6): 2274-2282.

Figures/Tables 10

Table 1 Effect and cellulose enzymatic digestibility of sugarcane bagasse after EPH pretreatment under different temperature

温度/℃	酸度/(mol/L)	时间/min	纤维素保留率/%	半纤维素去除率/%	木质素去除率/%	残渣保留率/%	酶解率/%
70	0.05	60	97.89	1.06	3.41	95.23	18.89
90	0.05	60	92.72	28.16	22.77	78.09	29.73
110	0.05	60	90.47	76.70	90.33	48.54	72.79
130	0.05	60	87.04	100.00	92.68	40.17	76.15

Table 2 Comparison of enzymatic hydrolysis of sugarcane bagasse with different organic solvent pretreatment

有机溶剂	催化剂	温度/℃	时间/min	酶解率/%	文献
乙二醇苯醚	硫酸	110	60	89.71	本文
乙二醇苯醚/丙酮/水	硫酸	125	120	74.52	[19]
60%乙醇	FeCl₃	160	60	93.80	[20]
水/乙醇/乙酸乙酯/甲酸	甲酸	159	40	84.50	[21]
甘油	硫酸	200	15	70.00	[22]

Fig.1 SEM images of the raw material and pretreated residue

Fig.2 FTIR spectrum of the raw material and residue

Fig.3 X-Ray diffraction patterns of the raw material and residue

Fig.4 Side chain region (δC/δH 45—95/2.5—6.0) and aromatic region (δC/δH 95—140/6.0—8.5) in 2D HSQC NMR spectra of CEL and EPHL

Fig.5 Main interunit linkages and lignin subunits of aromatic and side chain regions of CEL and EPHL

Table 3 Semi-quantitative analysis of aromatic and aliphatic regions in the 2D HSQC NMR spectra of CEL and EPHL

木质素结构单元和基本连接键	CEL	EPHL
S/%	30.78	20.96
G/%	69.22	79.04
S/G	0.445	0.265
β-O-4/%	53.82	19.71
β-β/%	7.12	4.41

Fig.6 FTIR spectra of the CEL and EPHL

Fig.7 Mass balance analysis of EPH pretreatment

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