The competitive relationship between PAOs-GAOs and N2O emission in An/A/O-SBR under different NO3- cultivation

doi:10.11949/0438-1157.20200829

Abstract

Abstract:

Using anaerobic/anoxic/aerobic sequencing batch reactors (An/A/O-SBRs), the long-term impact of the NO₃^- cultivation on denitrification phosphorus removal performance and N₂O emission characteristics was investigated in four SBRs of 10(R10), 20(R20), 30(R30) and 40 mg N/L(R40). The anaerobic and anoxic stoichiometric parameters related to various NO₃^- concentrations were monitored over time. The results showed that with the increasing of NO₃^- concentration, the total nitrogen (TN) removal efficiency decreased from more than 90% to 41.3%, and the N₂O emission ratio (N₂O emission/ NO_x^-_removal) were 1.68%, 4.17%, 8.92% and 14.28% respectively, while the total phosphorus removal (TP) efficiency increased at first and then decreased. NO₂^- accumulates in the process of high-concentration NO₃^- anoxic phosphorus absorption, which inhibits the activity of DPAOs, and the carbon source competition ability of GAOs is enhanced. The ratio of DPAOs/COD consumption in anaerobic process decreased from 33.5% to 25.1%, while that of GAOs increased from 59.3% to 74.1% as the NO₃^- increased from 10 to 40 mg N/L. The NO₂^- /HNO₂ accumulation coupled with the increase of GAOs, was the main reason for the reduction of nitrogen and phosphorus efficiencies and higher N₂O yields under higher NO₃^- concentration.

Key words: denitrifying phosphorus accumulating organisms, glycogen accumulating organisms, NO₃^-, N₂O, competitive mechanism

摘要：

采用厌氧/缺氧/好氧运行的序批式生物反应器（An/A/O-SBR），经不同NO₃^-浓度（10，20，30和40 mg/L，以氮计）长期驯化，考察了不同NO₃^-条件下An/A/O-SBR脱氮除磷及N₂O释放特性，基于不同微生物降解特性分析，确定了不同NO₃^-浓度下SBR系统内反硝化聚磷菌（denitrifying phosphorus accumulating organisms，DPAOs）和聚糖菌（glycogen-accumulating organisms， GAOs）竞争关系。结果表明：随NO₃^-浓度增加，总氮（TN）去除率由90%以上降至41.3%，TP去除率呈先增高后降低的趋势，N₂O产率（N₂O_emission/NO_x^-_removal）分别为1.68%、4.17%、8.92%和14.28%。An/A/O-SBR内微生物呈PAOs和GAOs共存的污染物降解特性，高浓度NO₃^-缺氧吸磷过程出现NO₂^-积累，抑制DPAOs活性，GAOs碳源竞争能力增强，NO₃^--N由10 mg/L增至40 mg/L，厌氧阶段PAOs的COD耗量比例由33.5%降至25.1%，相应GAOs的COD耗量由59.3%增至74.1%。DPAOs-GAOs共生体系内，反硝化过程NO₂^-/HNO₂积累耦合反硝化聚糖菌比例增加，加剧了高NO₃^-下An/A/O-SBR内N₂O释放。

关键词: 反硝化聚磷菌, 聚糖菌, 硝态氮, 氧化亚氮, 竞争机制

CLC Number:

GONG Youkui, LI Meiling, SUN Hongwei. The competitive relationship between PAOs-GAOs and N₂O emission in An/A/O-SBR under different NO₃^- cultivation[J]. CIESC Journal, 2021, 72(3): 1675-1683.

巩有奎, 李美玲, 孙洪伟. 不同NO₃^-浓度An/A/O-SBR系统PAOs-GAOs竞争及N₂O释放特性[J]. 化工学报, 2021, 72(3): 1675-1683.

Figures/Tables 6

Fig.1 Schematic diagram of the sequence batch reactor(SBR) and its operation mode

Fig.2 Variations of nitrogen (a) and PO43--P (b) under different NO3--N concentration in the An/A/O-SBRs

Fig.3 Variations of COD, NOx- and PO43--P during typical cycles under diferent NO3- in An/A/O-SBRs

Fig.4 COD consumption (a) and internal polymers variation (b) during anaerobic stage in An/A/O-SBRs

Fig.5 Internal polymers conversion and utilization during anoxic stage in An/A/O-SBR under different NO3-

Fig.6 Characterics of NO2--N, dissolved N2O (a) and N2O emission (b) during anoxic stage in An/A/O-SBRs

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The competitive relationship between PAOs-GAOs and N₂O emission in An/A/O-SBR under different NO₃^- cultivation

不同NO₃^-浓度An/A/O-SBR系统PAOs-GAOs竞争及N₂O释放特性

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