Cell growth and fatty acid production of heterotrophic microalgae Chlorella sp. cultivated in enzymatic hydrolyzate of sugarcane bagasse

doi:10.11949/j.issn.0438-1157.20150977

CIESC Journal ›› 2016, Vol. 67 ›› Issue (4): 1549-1556.DOI: 10.11949/j.issn.0438-1157.20150977

Cell growth and fatty acid production of heterotrophic microalgae Chlorella sp. cultivated in enzymatic hydrolyzate of sugarcane bagasse

WANG Wen¹, YANG Kang¹, ZHU Shunni¹, FENG Jia¹, SHANG Changhua¹, WANG Zhongming¹, YUAN Zhenhong^1,2, ZHUANG Xinshu¹, HU Lei³

1. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China;
2. Collaborative Innovation Center of Biomass Energy, Zhengzhou 450002, Henan, China;
3. Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huai'an 223300, Jiangsu, China

Received:2015-06-24 Revised:2015-11-09 Online:2016-04-05 Published:2016-04-05
Supported by:
supported by the National Basic Research Program of China (2011CB200905), the National Natural Science Foundation of China (51476177), The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ([2013] 693) and Open Foundation of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (JSBEET1316).

小球藻在蔗渣酶解液中的异养生长及其脂肪酸生成

王闻¹, 杨康¹, 朱顺妮¹, 冯佳¹, 尚常花¹, 王忠铭¹, 袁振宏^1,2, 庄新姝¹, 胡磊³

1. 中国科学院可再生能源重点实验室, 广东省新能源和可再生能源研究开发与应用重点实验室, 中国科学院广州能源研究所, 广东广州 510640;
2. 生物质能源河南省协同创新中心, 河南郑州 450002;
3. 淮阴师范学院, 江苏省生物质能与酶技术重点实验室, 江苏淮安 223300

通讯作者: 袁振宏
基金资助:
国家重点基础研究发展计划项目子课题(2011CB200905);国家自然科学基金项目(51476177);留学回国人员科研启动基金([2013]693号);江苏省生物质能与酶技术重点实验室开放课题基金(JSBEET1316)。

Abstract

Abstract:

After adding Tween80 into the enzymatic hydrolysis process, the glycan conversion of sugarcane bagasse (SCB) pretreated by liquid hot water (LHW) was improved 11.4%. The enzymatic hydrolyzates with and without Tween80 were prepared as media to cultivate heterotrophic Chlorella sp.. According to the compositional feature of enzymatic hydrolyzate, the synthetic media composed of glucose, xylose and cellobiose were prepared to mimic the media containing enzymatic hydrolyzate. The medium which only contained glucose was used as the positive control. The glucose concentration in all of the media was 10 g·L^-1. The synthetic media containing Tween80 or not were mixed with BG11 which did not contain carbonate, while the hydrolyzates media were prepared with or without the foregoing BG11. The cell growth and fatty acids yield of heterotrophic Chlorella sp. cultivated on the above media were investigated. The results showed that Tween80 could inhibit the cell growth of Chlorella sp., and cellobiose could also put negative impact on the biomass of Chlorella sp.. Chlorella sp. cultivated on medium containing glucose and BG11 attained the maximum biomass of 1.97 g·L^-1. The biomass of Chlorella sp. growing on enzymatic hydrolyzate media with BG11 was 2 times more than that on enzymatic hydrolyzate media without BG11. 6.90% of maximum fatty acid content was achieved when Chlorella sp. was cultured in enzymatic hydrolyzate media containing Tween80 and BG11. The chief fatty acids produced in all media were C_16:0, C_18:1, C_18:3, C_20:1and C_20:4. The addition of inorganic and organic nutrients into cellulolytic hydrolyzate can enhance the biomass and lipid content of microalgae.

Key words: microalgae, biomass, biocatalysis, heterotrophic cultivation, biodiesel, liquid hot water

摘要：

在高温液态水处理的甘蔗渣酶解过程中添加Tween80可使聚糖转化率提高11.4%。根据蔗渣酶解液中糖的种类及含量,用葡萄糖、木糖和纤维二糖标准品模拟蔗渣酶解液组成配制成相应的混合糖培养基,同时配制仅含葡萄糖的培养基,在有、无Tween80和BG11(Blue-Green 11)的条件下,考察小球藻在不同培养基中的异养生长及脂肪酸生成。结果显示Tween80对小球藻的生长具有抑制作用,纤维二糖也会影响小球藻的生长;小球藻在添加BG11的葡萄糖培养基中的生物量最高,为1.97 g·L^-1,在添加BG11的蔗渣酶解液中的生物量高出未添加BG11的2倍,在含有Tween80和BG11的蔗渣酶解液中的总脂肪酸含量最高,达到6.90%,在所有培养基中产生的脂肪酸以C_16:0、C_18:1、C_18:3、C_20:1和C_20:4为主;培养基组成优化可进一步提高微藻生物量和油脂产量。

关键词: 微藻, 生物质, 生物催化, 异养培养, 生物柴油, 高温液态水

CLC Number:

WANG Wen, YANG Kang, ZHU Shunni, FENG Jia, SHANG Changhua, WANG Zhongming, YUAN Zhenhong, ZHUANG Xinshu, HU Lei. Cell growth and fatty acid production of heterotrophic microalgae Chlorella sp. cultivated in enzymatic hydrolyzate of sugarcane bagasse[J]. CIESC Journal, 2016, 67(4): 1549-1556.

王闻, 杨康, 朱顺妮, 冯佳, 尚常花, 王忠铭, 袁振宏, 庄新姝, 胡磊. 小球藻在蔗渣酶解液中的异养生长及其脂肪酸生成[J]. 化工学报, 2016, 67(4): 1549-1556.

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Cell growth and fatty acid production of heterotrophic microalgae Chlorella sp. cultivated in enzymatic hydrolyzate of sugarcane bagasse

小球藻在蔗渣酶解液中的异养生长及其脂肪酸生成

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